A smart ZnO@polydopamine-nucleic acid nanosystem for ultrasensitive live cell mRNA imaging by the target-triggered intracellular self-assembly of active DNAzyme nanostructures

He, Dinggeng; He, Xiyun; Yang, Xue; Li, Hung‐Wing

doi:10.1039/c6sc04633a

Cited by 91 publications

(76 citation statements)

References 45 publications

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“…A large pool of HeLa cells was treated with YM155 in different concentrations to decrease the intracellular survivin mRNA expression to mimic the natural change of mRNA expression upon biological stimulus. 40 The fluorescence signals were amplified with DCR and HCR in cytoplasm for comparison. The bright fluorescence was still observed from cells treated with 3.0 nM YM155 for DCR signal amplification, while the cells treated with YM155 at the same concentrations demonstrated indistinguishable fluorescence signals for HCR signal amplification ( Figure 5).…”

Section: Feasibility Ofmentioning

confidence: 99%

A Responsive “Nano String Light” for Highly Efficient mRNA Imaging in Living Cells via Accelerated DNA Cascade Reaction

et al. 2017

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Nonenzymatic DNA catalytic amplification strategies have greatly benefited bioanalysis. However, long period incubation is usually required due to its relatively low reaction rate and efficiency, which limits its in vivo application. Here we design a responsive DNA nano string light (DNSL) by interval hybridization of modified hairpin DNA probe pairs to a DNA nanowire generated by rolling circle amplification and realize accelerated DNA cascade reaction (DCR) for fast and highly efficient mRNA imaging in living cells. Target mRNA initiates interval hybridization of two paired hairpin probes sequentially along the DNA nanowire and results in instant lighting up of the whole DNA nanowire with high signal gain due to the fast opening of all the self-quenched hairpins. The reaction time is about 6.7 times shorter compared with a regular DNA cascade reaction due to the acceleration based on domino effect. The cell delivery is achieved by modifying one of the hairpin probes with folic acid, and this intracellular imaging strategy is verified using human HeLa cells and intracellular survivin mRNA with a series of suppressed expressions as model, which provides a useful platform for fast and highly efficient detection of low-abundance nucleic acids in living cells.

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Section: Feasibility Ofmentioning

confidence: 99%

A Responsive “Nano String Light” for Highly Efficient mRNA Imaging in Living Cells via Accelerated DNA Cascade Reaction

et al. 2017

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“…49,50 Both the catalytic DNA circuit and functional DNAzyme are utilized for intracellular miRNA imaging. 51,52 Yet these individual ampliers are always confronted with limited versatilities and unsatisfactory signal gains, which could be solved by their integration with other amplication strategies. [53][54][55] The in-depth integration of these different amplication methods attracts more attention since a higher signal gain is expected to be realized in live cell analysis.…”

Section: Introductionmentioning

confidence: 99%

A DNAzyme-amplified DNA circuit for highly accurate microRNA detection and intracellular imaging

Hong

Wang

et al. 2019

Chem. Sci.

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“…He et al developed probes employing a dual amplification strategy for the detection of mRNA in live cells . These probes consisted of a ZnO core onto which four hairpin DNA strands were adsorbed.…”

Section: Dnazyme‐based Probesmentioning

confidence: 99%

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

2019

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The chemical composition of cells at the molecular level determines their growth, differentiation, structure, and function. Probing this composition is powerful because it provides invaluable insight into chemical processes inside cells and in certain cases allows disease diagnosis based on molecular profiles. However, many techniques analyze fixed cells or lysates of bulk populations, in which information about dynamics and cellular heterogeneity is lost. Recently, nucleic‐acid‐based probes have emerged as a promising platform for the detection of a wide variety of intracellular analytes in live cells with single‐cell resolution. Recent advances in this field are described and common strategies for probe design, types of targets that can be identified, current limitations, and future directions are discussed.

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A smart ZnO@polydopamine-nucleic acid nanosystem for ultrasensitive live cell mRNA imaging by the target-triggered intracellular self-assembly of active DNAzyme nanostructures

Abstract: We have developed a smart ZnO@PDA-hpDNAs nanosystem carrying four functional hpDNA strands that can realize the target-triggered self-assembly of wire-shaped active DNAzyme nanostructures via HCR in live cells.

Cited by 91 publications

References 45 publications

A Responsive “Nano String Light” for Highly Efficient mRNA Imaging in Living Cells via Accelerated DNA Cascade Reaction

A Responsive “Nano String Light” for Highly Efficient mRNA Imaging in Living Cells via Accelerated DNA Cascade Reaction

A DNAzyme-amplified DNA circuit for highly accurate microRNA detection and intracellular imaging

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

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