A solvent-assisted ESIPT fluorescent dye for F−/Ag+ sensing and high-resolution imaging of the cilia in live cells

ESIPT-based probes for cations, anions and neutral species: recent progress, multidisciplinary applications and future perspectives

“…Solvent-dependent ESIPT properties were exploited by Gong et al 37 using pyrene-based turn-off probe 4 for F − /Ag + in EtOH/ HEPES solution (3/1, 10.0 mM, pH 7.2) (Fig. 4).…”

Design and Synthesis of ESIPT-Based Imidazole Derivatives for Cell Imaging

“…Fluorescent-based imaging agents have several applications and several distinct emission mechanisms, one of which is excited-state intramolecular proton transfer (ESIPT) emission. − ESIPT emission covers a unique mechanism that results in two distinct emission bands in most cases. , Large Stokes shift, possible biocompatibility, high cell penetration ability, and phototautomerization in excited state make it a more valuable candidate for special imaging processes including lysosome, DNA, and nucleus. − …”

Ti3C2Tx MXene Nanosheet-Based Probe for Ion Fluorescence and Visual Detection of Ag+ in Aqueous Solution and Living Cells

“…A series of detection methods have been developed to detect silver ions, such as atomic absorption spectroscopy, inductively coupled plasma–mass spectroscopy, and ion-selective electrodes. , However, these methods mentioned above all require expensive equipment or complex technology. − On this basis, fluorescent probes have become a popular method due to their high sensitivity, rapidity, simple operation, common instruments, and suitability for biological systems. − In the past years, some fluorescent probes for detecting silver ions have been developed, but the following defects are still not solved. − First, some currently reported methods utilize the “silent ion” of silver ions to quench their fluorescence, which is not conducive to high signal output. , Second, the proposed probes only show moderate fluorescence changes, small response range to silver ion concentration, so the sensitivity of these probes is usually not enough to detect low concentrations of silver ions in water samples. , Finally, the probes reported in the literature are difficult to distinguish Ag + from other metal ions (such as Au 3+ ). , Therefore, it is an urgent need to develop a novel fluorescent probe with high sensitivity and selectivity in real samples. − …”

“…Similarly, the good biological characteristics of MXene are seldom reflected. At present, the existing design idea of a silver ion probe is that the nanomaterials themselves have certain fluorescence characteristics, ,,, which is not conducive to further development and modification by subsequent researchers. Based on the mechanism of silver nanoparticle replaces R6G on the surface of MXene and releases R6G for detection, different substances can be detected by changing the surface properties of MXene or changing the dye molecules.…”