A Specific Histochemical Marker (Lectin Ricinus Communis Agglutinin-1) for Normal Human Microglia

Mannoji, Hiromichi; Yeger, Herman; Becker, Laurence E.

doi:10.1097/00005072-198605000-00095

Cited by 46 publications

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“…On the other hand, positive cells for lectin RCA-1, another macrophages/histiocytes marker, was intensely detected in both diseases. Lectin RCA-1 is originally employed for detecting microglia [16], and the cells are presumably derived from bone marrow and play histiocyte-like roles in CNS [1]. Microglias can be distinguished from infiltrative macrophages by their characteristic ramified morphology and their localization in normal CNS.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Pathological Study on Canine Necrotizing Meningoencephalitis and Granulomatous Meningoencephalomyelitis

Suzuki

Uchida

Morozumi

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. Canine necrotizing meningoencephalitis (NME) and granulomatous meningoencephalomyelitis (GME) were compared pathologically. Gross observation exhibited lateral ventricular dilation and discoloration, malacia and/or cavitation of the cerebrum in NME. On the contrary, gross changes were milder in GME, except for occasional visible granulomatous mass formation. Histopathologically, the lesions of NME were distributed predominantly in the cerebral cortex and various degrees of inflammatory and necrotic changes were observed according to clinical stages. Besides, microscopic lesions of GME were mainly distributed in the white matter of the c erebrum, cerebellum and brainstem, which are characterized by perivascular cuffing, multiple granulomas and leptomeningeal infiltrates. Although macrophages and lymphocytes were predominant in the inflammatory lesions of both disorders, macrophages in GME transformed into epithelioid cells and exhibited more massive infiltration. Although lectin RCA-1-reactive cells were numerous in both disorders, lysozyme immunoreactive cells in NME were fewer than that in GME. Parenchymal infiltration of MAC387-positive cells was common in GME and limited in NME. The number of CD3-positive lymphocytes in the GME lesions tended to be greater than in NME, though the difference was not statistically significant. Morphological and immunohistochemical differences of the lesions, in particular, the characteristics of infiltrative macrophages may reflect these different pathogeneses of the two disorders. KEY WORDS: canine, granulomatous meningoencephalomyelitis, macrophage, necrotizing meningoencephalitis.J. Vet. Med. Sci. 65(11): 1233-1239, 2003 Canine necrotizing meningoencephalitis (NME) is a unique inflammatory disorder in small-sized breed dogs, especially in Pug dogs. The disease is histopathologically characterized by inflammatory changes consisting of lymphocytic, plasmacytic and histiocytic infiltrations and apparent parenchymal necrosis located mainly in the cerebral cortex [9,15,23]. The common clinical features are forebrain signs such as partial or generalized seizure, decreased consciousness, abnormal behavior, circling and ataxia [9,23]. The cause of NME is still unknown. However, our previous report showed that a certain autoantibody against a canine brain tissue was detected in the cerebrospinal fluid (CSF) and serum, which may suggest an autoimmune pathology in NME [25].The pathological features of NME are often compared with granulomatous meningoencephalomyelitis (GME) that is another inflammatory disease of unknown cause [5,8,23]. Although there are some differences such as breed predilection, the distribution of lesions and the presence or absence of necrotic foci, GME and NME show similar histological changes, i.e., meningitis and perivascular cuffing composed of mononuclear cells including lymphocytes and monocyte/histiocyte-lineages [5,8,23]. Kipar et al. [14] revealed lesions in GME are predominantly composed of CD3 antigen-positive T lymphocytes and a heterogeneo...

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Section: Discussionmentioning

confidence: 99%

A Comparative Pathological Study on Canine Necrotizing Meningoencephalitis and Granulomatous Meningoencephalomyelitis

Suzuki

Uchida

Morozumi

et al. 2003

The Journal of Veterinary Medical Science

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“…On the contrary, lectin RCA-1 was the most sensitive to inflammatory and neoplastic histiocytes of all histiocytic markers in the present study. Although there have been few reports to employ for canine tissue, it is well known that RCA-1 is a good marker for detecting microglia and macrophage in human brain [8,14]. Antibodies to MAC387 and CD68, human histiocytic markers [5,6,15,18,23], labeled very limited number of canine histiocytes.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Pathological Study on Granulomatous Meningoencephalomyelitis and Central Malignant Histiocytosis in Dogs

Suzuki

Uchida

Morozumi

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. Histiocytic proliferative disorders in canine central nervous system (CNS) including granulomatous meningoencephalomyelitis (GME) and malignant histiocytosis were compared pathologically. Lesions of GME mainly existed in the white matter of the cerebr um, brainstem and cerebellum and consisted of characteristic perivascular cuffing, parenchymal granuloma and leptomeningeal infiltrates of mononuclear cells. In malignant histiocytosis, there were two histological patterns, diffuse proliferation of neoplastic histiocytes through the leptomeninges and neoplastic nodule formation in the parenchyma. Neoplastic histiocytes exhibited mild to severe cellular atypia and high ability of invasion into the brain parenchyma. Mitotic and phagocytic figures were also observed. Several histiocytic markers, including lysozyme, α1-antitrypsin and lectin RCA-1, revealed histiocytic origin of both inflammatory and neoplastic cells, however, those were not determinative for the discrimination between GME and malignant histiocytosis. CD3-and PCNA-positive cells existed in the lesions of both diseases. The number of CD3-positive cells in GME tended to be greater than in malignant histiocytosis, while the difference was not statistically significant.

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“…Further, PNA stained most small neurons in sensory ganglia and myelinated fibers in the white matter of the spinal cord (Streit et al, 1985), whereas Vicia villosa lectin with a specificity for terminal N-acetylgalactosamine reacted with most GABAergic neurons in the thalamic reticular nucleus of rodent brains (Nakagawa et al, 1986). In neuroglia , RCA-1 and Griffonia simplicifolia lectin have been presumed as a microglial cell marker (Mannoji et al, 1986; Boya et al, 1991). In addition, receptors with different functions have been reported to show affinity towards various lectins in mammalian nervous system.…”

Section: Discussionmentioning

confidence: 99%

Lectin-Binding Glycoconjugates in the Subfornical Organ of the Rat

Ueda

Fujimori

Yamada

1997

Okajimas Folia Anatomica Japonica

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Summary: In the rat subfornical organ (SFO), lectin-binding glycoconjugates were histochemically examined by 9 biotinylated lectins using a streptavidin-biotin peroxidase system. The strong or moderate bindings of Canavalia ensiformis (Con A), Lens culinaris (LCA) and Phaseolus vulgaris erythroagglutinin (ePHA) indicated overall distributions of high mannose, intermediate, hybrid N-linked, and non-bisected and bisected, bi/triantennate N-linked complex oligosaccharides in the rat SFO. Nonsialylated terminal N-acetylglucosamines were detected throughout this organ, as revealed by its stainabilities with Triticum vulgaris (WGA) and Limax flavus (LFA). In this organ, Ricinus communis (RCA-1) specifically bound to vessel-associated structures, whereas Arachis hypogaea (PNA) reacted with selected neurons in the central and rostral regions of this organ. Dolichos biflorus (DBA) and Ulex europaeus (UEA-1) did not stain any histologic structures in the rat SFO. The results obtained in this study provide a basis for comprehensive analyses of glycoconjugates in the rat SFO.

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A Specific Histochemical Marker (Lectin Ricinus Communis Agglutinin-1) for Normal Human Microglia

Cited by 46 publications

References 0 publications

A Comparative Pathological Study on Canine Necrotizing Meningoencephalitis and Granulomatous Meningoencephalomyelitis

A Comparative Pathological Study on Canine Necrotizing Meningoencephalitis and Granulomatous Meningoencephalomyelitis

A Comparative Pathological Study on Granulomatous Meningoencephalomyelitis and Central Malignant Histiocytosis in Dogs

Lectin-Binding Glycoconjugates in the Subfornical Organ of the Rat

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