A specific histochemical method for phospholipids and sulpholipids

Kennedy, G. Y.; Soper, Rosemary; Lunn, Daniel

doi:10.1007/bf01003540

Cited by 6 publications

(1 citation statement)

References 2 publications

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“…thick, were cut in a microtome cryostat at -25°C , were air-dried on to slides and stained for one of the following lipids, using the necessary controls : neutral lipids, using oil red O (Chayen, 1968;Pearse, 1968); unsaturated lipids, using the brominesilver technique (Norton, Korey & Brotz, 1962); phospholipids using the method of Kennedy, Soper & Lunn (1970) ; and free fatty acids, using Fischler's method (Luna, 1968), and counterstained as required.…”

Section: Methodsmentioning

confidence: 99%

Effects of Ovarian Steroid Hormones on Histochemically Demonstrable Lipids in the Rat Uterine Epithelium

Boshier¹,

Holloway²

1973

Journal of Endocrinology

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Histochemical studies showed that during the rat oestrous cycle, uterine epithelial lipids were at their highest levels during dioestrus and pro-oestrus. In ovariectomized mature animals given oestradiol-17\g=b\, either by itself or in combination with medroxyprogesterone acetate, neutral lipids and unsaturated lipids were below control levels, while phospholipids were increased. Medroxyprogesterone acetate, however, increased levels of neutral lipids and unsaturated lipids, and decreased phospholipids. It is suggested that during the oestrous cycle of the rat, high concentrations of uterine epithelial lipid during late dioestrus and pro-oestrus reflect the increased plasma progesterone of early dioestrus acting in a low plasma oestrogen environment.

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Section: Methodsmentioning

confidence: 99%