A specific oligonucleotide of the 5S rDNA spacer and species-specific elements identify symmetric somatic hybrids between Solanum tuberosum and S. pinnatisectum

Zanke, C.; Borisjuk, Nikolai; Ruoss, B.; Schilde-Rentschler, L.; Ninnemann, Helga; Hemleben, Vera

doi:10.1007/bf00222139

Cited by 28 publications

(11 citation statements)

References 25 publications

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“…The beginning Genomic region analyzed of the coding region generally shows some family specificity (Volkov et al, 2001), such as GGA (or TGA) in Graminae (Baum and Johnson, 1996), GGG in Gossypium (Cronn et al, 1996), AGG in Legume (Gottolob-McHugh et al, 1990) and GGA in Solanum (Zanke et al, 1995). In other species such as Hordeum and Vigna, the coding region ended with GTG (or TCC) and CCT, respectively (Baum and Johnson, 1996;Saini and Jawali, 2009).…”

Section: Resultsmentioning

confidence: 97%

Organization of the 5S rRNA gene units in Korean Lilium species

2011

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The 5S rRNA gene unit was characterized from Lilium species distributed in Korea. Sequence analyses revealed that the 5S rDNA, harboring a highly conserved coding region (120 bp) and a divergent non transcribed spacer (NTS), ranged from 470 bp to 640 bp. The NTS regions showed length heterogeneity (350 bp-520 bp) due to the presence of several indels, and extensive sequence divergence among the species. Despite heterogeneity in length and nucleotide composition, the NTS regions possess some common features across the species, which include a T stretch region CTTTT and an identical motif of 11 bp (CAATGTATGAC) at the 3′ and 5′ flanking sequence of the coding region, respectively. These may play a role in the initiation and termination of transcription. The chromosomal distribution of the 5S rRNA gene was mapped on the long arm of chromosome 3 by fluorescence in situ hybridization (FISH). The phylogenetic analysis based on the NTS sequence broadly divided the Lilium species into two major groups which were referred to as the section Sinomartagon and Martagon by Comber's classification of the genus Lilium. The present study showed that the 5S rDNA sequence was very useful to unravel the genetic relationships among Lilium species.

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Section: Resultsmentioning

confidence: 97%

Organization of the 5S rRNA gene units in Korean Lilium species

2011

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“…Total DNA was isolated from calli and plants regenerated in vitro according to Doyle et al (1990). Two primers p 5S1 (5'-GGATGGGTGACCTCCCGGGAAGTCC-3') and p 5S2 (5'-CGCTTAACTGCGGAGTTCTGATGGG-3') (Zanke et al 1995) were used for ampli®cation of the 5S rDNA spacer sequence. PCR was carried out in a Perkin Elmer System 4800.…”

Section: Analysis Of Nuclear Dna By Pcrmentioning

confidence: 99%

Analysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestiuvm and Haynaldia villosa Schur

et al. 2001

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Intergeneric somatic hybridization between wheat (cv. Jinan 177) protoplasts that have 24-28 chromosomes and Haynaldia villosa protoplasts containing 11-14 chromosomes was carried out by the polyethylene glycol (PEG) method. A high frequency of hybrid calli and plants were obtained from the fusion products, as revealed by cytological and biochemical techniques and by PCR analysis of 5S rDNA spacer sequences. GISH (genomic in situ hybridization) analysis confirmed the presence of chromosomes from both parents in the hybrid clones and the common occurrence of translocations between them. The RFLP analysis of the organellar DNA using mitochondrion- and chloroplast-specific probes revealed that mitochondria from both parents existed in the cells of hybrid calli and their recombination, whereas chloroplasts segregated and recombined randomly. The gross morphology of hybrid plants resembled that of wheat, but the gross morphology of their ovaries and anthers were intermediate between those of the two parents. The relationship between hybrid plant regeneration and the balance of genetic materials in hybrid clones is discussed.

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“…Variations in the NTS of the 5S rDNA owing to insertions/deletions, minirepeats and pseudogenes have been useful for evolutionary studies and served as species-or population-specific markers for various organisms, including plants (Solanum tuberosum and Solanum pinnatisectum) (Zanke et al, 1995), mammals (Mus musculus domesticus and Mus musculus musculus) (Suzuki et al, 1994) and fish (Oncorhynchus mykiss, Salmo salar, Salmo truta, Brama raii, Solea solea, Reinhardtius hippoglossoides, Anguilla anguilla, Anguilla rostrata and Oreochromis niloticus) (Pendás et al, 1995, Nieddu et al, 1998, Céspedes et al, 1999, Carrera et al, 2000Martins et al, 2002). Variations in the 5S rDNA non-transcribed spacer have also been detected in some Neotropical fish such as Leporinus (Martins and Galetti, 2001a) and Brycon (Wasko et al, 2001) species.…”

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes)

et al. 2006

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The 5S ribosomal DNA (5S rDNA) of higher eukaryotes is organized in repeat units of tandem arrays composed of a 5S rDNA coding region, conserved even among non-related taxa, and a variable non-transcribed spacer sequence (NTS). To contribute to knowledge on the organization and evolution of vertebrate 5S rDNA we used PCR, nucleotide sequencing, Southern blot hybridization and chromosome fluorescence in situ hybridization (FISH) to investigate 5S rDNA tandem repeats in the South American Curimatidae fish Steindachnerina insculpta and Cyphocharax modesta. 5S rDNA repeats of 180 base pairs (bp) from both species were PCR-generated and sequenced evidencing the shortest 5S rDNA monomer so far described in eukaryote species. Southern blotting revealed that both species contained two tandem 5S rDNA classes, the PCR amplified fragment composed of 180 bp monomers and a class of 1600 bp monomers not detected by PCR. Chromosome mapping of the 5S rDNA repeats identified a major locus in both species and a second minor locus only in C. modesta. The Southern blot and chromosome mapping data indicate the presence of different types of 5S rDNA tandem repeats in the Curimatidae genome.

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A specific oligonucleotide of the 5S rDNA spacer and species-specific elements identify symmetric somatic hybrids between Solanum tuberosum and S. pinnatisectum

Cited by 28 publications

References 25 publications

Organization of the 5S rRNA gene units in Korean Lilium species

Organization of the 5S rRNA gene units in Korean Lilium species

Analysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestiuvm and Haynaldia villosa Schur

Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes)

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