A specific, sensitive assay of glycerol and l-α-glycerophosphate

Boltralik, J. J.; Noll, Hans

doi:10.1016/0003-2697(60)90054-3

Cited by 23 publications

(2 citation statements)

References 4 publications

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“…Citrate was determined by the method of Saffron and Denstedt (1948). Glycerol was determined by the periodate method of Dyer (1956) or by the enzyme method of Boltralik and Noll (1960), as specified in the text. It was necessary to use the enzymatic method for determining glycerol when glucose was present in the medium, since both constituents are determined by the periodate method.…”

Section: Methodsmentioning

confidence: 99%

Kinetics of Utilization of Organic Compounds in the Growth ofMycobacterium tuberculosis

Bowles

Segal

1965

J Bacteriol

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AND WILLIA SEGAL. Kinetics of utilization of organic compounds in the growth of Mycobacterium tuberculosis. J. BACTERIOL. 90:157-163. 1965.-To obtain a workable system for a study of the kinetics of nutrient utilization (based on specific quantitative assay) by Mycobacterium tuberculosis, several cultural refinements were introduced: the use of shake culture, a 40-fold increase in the size of inoculum, substitution of glutamate for asparagine as nitrogen source, and elimination of glucose from the medium with glycerol remaining as carbon source. These modifications resulted in reduction to a tenth of the lag phase of glycerol utilization (from 40 to 4 days), and in a greatly increased rate of growth. Both coordinate and sequential patterns of nutrient utilization were in evidence, except in the case of citrate, which was never utilized under a variety of conditions of culture. The coordinate pattern of glucose-glutamate and glucose-glycerol utilization would appear to rule out catabolite repression by glucose. However, elimination of glucose from the medium resulted in elimination of the 4-day lag period before glutamate utilization was initiated, leaving open to question the role of glucose in this system. Evidence is presented for the hypothesis that the sequential pattern of glutamate-glycerol utilization is a function of glutamate repression of glycerol oxidation in the growth of M. tuberculosis, although no diauxie effect is apparent. In a determination of which nutrient-utilization systems were regulated by induction, only in the case of glycerol was evidence obtained for an inducible system. The enzymatic mechanisms underlying these patterns of nutrient utilization are presently being investigated.

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Section: Methodsmentioning

confidence: 99%

Kinetics of Utilization of Organic Compounds in the Growth ofMycobacterium tuberculosis

Bowles

Segal

1965

J Bacteriol

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“…The use of enzymatic methods to analyze for ethanol and glycerol in beverages was proposed by Mayer (159). Drawert and Kupfer (45) and Boltralik and Noll (26) determined glycerol in wine and grape based on the esterification of glycerol to glycerol-l-phosphate with adenosine triphosphate in the presence of glycerol kinase. Hagen and Hagen (87) estimated the glycerol content in blood using glycerol dehydrogenase.…”

Section: Determination Of Enzymesmentioning

confidence: 99%