A SPECT Scanner for Rodent Imaging Based on Small-Area Gamma Cameras

Lage, Eduardo; Villena, Jose L.; Tapias, G.; Martinez, Naira P.; Soto-Montenegro, María Luisa; Abella, Mónica; Sisniega, Alejandro; Pino, Francisco; Ros, Domènec; Pavı́a, Javier; Vaquero, J.J.

doi:10.1109/tns.2010.2057516

Cited by 5 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Then, reconstruction was performed using filtered back projection algorithm with a ramp filter. A 128 × 128 × 100 matrix size and 0.32 × 0.32 × 0.32 mm 3 voxel size were used .…”

Section: Methodsmentioning

confidence: 99%

“…Then, reconstruction was performed using filtered back projection algorithm with a ramp filter. A 128 × 128 × 100 matrix size and 0.32 × 0.32 × 0.32 mm 3 voxel size were used (41). MRI was automatically realigned using Statistical Parametric Mapping (SPM, The Wellcome Department of Imaging Neuroscience, London, UK; www.fil.ion.ucl.ac.uk/spm) to the reference MRI, which was coregistered to the Rubins rat brain atlas (42).…”

Section: Spect Image Quantificationmentioning

confidence: 99%

See 1 more Smart Citation

In vivo evaluation of the dopaminergic neurotransmission system using [¹²³I]FP‐CIT SPECT in 6‐OHDA lesioned rats

Niñerola‐Baizán

Rojas

Bonastre

et al. 2014

Contrast Media & Molecular

Self Cite

View full text Add to dashboard Cite

The 6-hydroxydopamine (6-OHDA) rodent model of Parkinson's disease (PD) has been used to evaluate the nigrostriatal pathway. The aim of this work was to explore the relationship between the degree of 6-OHDA-induced dopaminergic degeneration and [(123)I]FP-CIT binding using single photon emission computed tomography (SPECT). Fourteen rats received a 6-OHDA injection (4 or 8 µg) into the left medial forebrain bundle. After 3 weeks, magnetic resonance imaging and scans with a small-animal SPECT system were performed. Finally, the nigrostriatal lesion was assessed by immunohistochemical analysis. Immunohistochemical analysis confirmed two levels of dopaminergic degeneration. Lesions induced by 6-OHDA diminished the ipsilateral [(123)I]FP-CIT binding by 61 and 76%, respectively. The decrease in tracer uptake between control and lesioned animals was statistically significant, as was the difference between the two 6-OHDA lesioned groups. Results concluded that [(123)I]FP-CIT SPECT is a useful technique to discriminate the degree of dopaminergic degeneration in a rat model of PD.

show abstract

“…Then, reconstruction was performed using filtered back projection algorithm with a ramp filter. A 128 × 128 × 100 matrix size and 0.32 × 0.32 × 0.32 mm 3 voxel size were used .…”

Section: Methodsmentioning

confidence: 99%

Section: Spect Image Quantificationmentioning

confidence: 99%

In vivo evaluation of the dopaminergic neurotransmission system using [¹²³I]FP‐CIT SPECT in 6‐OHDA lesioned rats

Niñerola‐Baizán

Rojas

Bonastre

et al. 2014

Contrast Media & Molecular

Self Cite

View full text Add to dashboard Cite

show abstract

“…FBP and 2D-iterative reconstruction algorithms used in the reconstruction of simulated studies were adapted versions of algorithms previously developed for clinical cameras (Falcon 1999, Pareto et al 2003 and satisfactorily employed in another small animal scanner (Lage et al 2010).…”

Section: Reconstructionmentioning

confidence: 99%

Quantification of rat brain SPECT with¹²³I-ioflupane: evaluation of different reconstruction methods and image degradation compensations using Monte Carlo simulation

et al. 2014

View full text Add to dashboard Cite

SPECT studies with (123)I-ioflupane facilitate the diagnosis of Parkinson's disease (PD). The effect on quantification of image degradations has been extensively evaluated in human studies but their impact on studies of experimental PD models is still unclear. The aim of this work was to assess the effect of compensating for the degrading phenomena on the quantification of small animal SPECT studies using (123)I-ioflupane. This assessment enabled us to evaluate the feasibility of quantitatively detecting small pathological changes using different reconstruction methods and levels of compensation for the image degrading phenomena. Monte Carlo simulated studies of a rat phantom were reconstructed and quantified. Compensations for point spread function (PSF), scattering, attenuation and partial volume effect were progressively included in the quantification protocol. A linear relationship was found between calculated and simulated specific uptake ratio (SUR) in all cases. In order to significantly distinguish disease stages, noise-reduction during the reconstruction process was the most relevant factor, followed by PSF compensation. The smallest detectable SUR interval was determined by biological variability rather than by image degradations or coregistration errors. The quantification methods that gave the best results allowed us to distinguish PD stages with SUR values that are as close as 0.5 using groups of six rats to represent each stage.

show abstract

“…Al igual que en el capítulo anterior, se han añadido un anexo (Anexos 5.1) que contiene una comparativa del sistema SPECT desarrollado con otros sistemas comerciales. Este capítulo está basado en Lage et al, 2009d;Lage et al, 2009e;Lage et al, 2009f).…”

Section: Aunque En Los úLtimos Años Grandes Casas Comerciales Como Ge...unclassified

Sistemas tomográficos multimodalidad para animales de laboratorio

Negro¹

View full text Add to dashboard Cite

A SPECT Scanner for Rodent Imaging Based on Small-Area Gamma Cameras

Cited by 5 publications

References 35 publications

In vivo evaluation of the dopaminergic neurotransmission system using [¹²³I]FP‐CIT SPECT in 6‐OHDA lesioned rats

In vivo evaluation of the dopaminergic neurotransmission system using [¹²³I]FP‐CIT SPECT in 6‐OHDA lesioned rats

Quantification of rat brain SPECT with¹²³I-ioflupane: evaluation of different reconstruction methods and image degradation compensations using Monte Carlo simulation

Sistemas tomográficos multimodalidad para animales de laboratorio

Contact Info

Product

Resources

About

A SPECT Scanner for Rodent Imaging Based on Small-Area Gamma Cameras

Cited by 5 publications

References 35 publications

In vivo evaluation of the dopaminergic neurotransmission system using [123I]FP‐CIT SPECT in 6‐OHDA lesioned rats

In vivo evaluation of the dopaminergic neurotransmission system using [123I]FP‐CIT SPECT in 6‐OHDA lesioned rats

Quantification of rat brain SPECT with123I-ioflupane: evaluation of different reconstruction methods and image degradation compensations using Monte Carlo simulation

Sistemas tomográficos multimodalidad para animales de laboratorio

Contact Info

Product

Resources

About

In vivo evaluation of the dopaminergic neurotransmission system using [¹²³I]FP‐CIT SPECT in 6‐OHDA lesioned rats

In vivo evaluation of the dopaminergic neurotransmission system using [¹²³I]FP‐CIT SPECT in 6‐OHDA lesioned rats

Quantification of rat brain SPECT with¹²³I-ioflupane: evaluation of different reconstruction methods and image degradation compensations using Monte Carlo simulation