In this paper, we introduce and study non-local Jacobi operators, which generalize the classical (local) Jacobi operator. We show that these operators extend to the generator of an ergodic Markov semigroup with a unique invariant probability measure and study its spectral and convergence properties. In particular, we give a series expansion of the semigroup in terms of explicitly defined polynomials, which are counterparts of the classical Jacobi orthogonal polynomials. In addition, we give a complete characterization of the spectrum of the non-self-adjoint generator and semigroup. We show that the variance decay of the semigroup is hypocoercive with explicit constants, which provides a natural generalization of the spectral gap estimate. After a random warm-up time, the semigroup also decays exponentially in entropy and is both hypercontractive and ultracontractive. Our proofs hinge on the development of commutation identities, known as intertwining relations, between local and non-local Jacobi operators/semigroups, with the local Jacobi operator/semigroup serving as a reference object for transferring properties to the non-local ones.