A Spin-Labeled Abasic DNA Substrate for AP Endonuclease

Kolaczkowski, Stephen V.; Perry, Alex; McKenzie, Andres; Johnson, Francis; Budil, David E.

doi:10.1006/bbrc.2001.5827

Cited by 9 publications

(2 citation statements)

References 16 publications

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“…This strategy uses aromatic ethers or fluorine as leaving groups and amino groups in spin labels, such as 4-amino-TEMPO , as nucleophiles (Figure ). − Both pyrimidine and purine analogues have been described, and the approach has been employed in several studies involving RNA and DNA. − …”

Section: General Overview Of Natural and Chemically Generated Paramag...mentioning

confidence: 99%

Paramagnetic Chemical Probes for Studying Biological Macromolecules

et al. 2022

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Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent years witnessed a great increase in the variety of probes for the study of biological macromolecules (proteins, nucleic acids, and oligosaccharides). This Review aims to provide a comprehensive overview of the existing paramagnetic chemical probes, including chemical synthetic approaches, functional properties, and selected applications. Recent developments have seen, in particular, a rapid expansion of the range of lanthanoid probes with anisotropic magnetic susceptibilities for the generation of structural restraints based on residual dipolar couplings and pseudocontact shifts in solution and solid state NMR spectroscopy, mostly for protein studies. Also many new isotropic paramagnetic probes, suitable for NMR measurements of paramagnetic relaxation enhancements, as well as EPR spectroscopic studies (in particular double resonance techniques) have been developed and employed to investigate biological macromolecules. Notwithstanding the large number of reported probes, only few have found broad application and further development of probes for dedicated applications is foreseen.

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Section: General Overview Of Natural and Chemically Generated Paramag...mentioning

confidence: 99%

Paramagnetic Chemical Probes for Studying Biological Macromolecules

et al. 2022

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“…A number of these have been applied to various curve fitting problems involving EPR spectra: simplex in powder patterns and orientational distribution of rigid samples [13,14], Levenberg-Marquardt for dynamically averaged line shapes [15], and simulations in the slow motional regime [16]. At the end of an optimization it is desirable to be able to test for the goodness of fit, a number of such tests is available, for example the reduced v 2 = v 2 /(n À p), with n the number of data points and p the number of fitted parameters, which for a fit in which the only deviation between model and data is Gaussian noise, is distributed about unity with a standard deviation of ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi ffi 2=ðn À pÞ p .…”

Section: Introductionmentioning

confidence: 99%