A Spin-Valve GMR Based Sensor with Magnetite@silver Core-Shell Nanoparticles as a Tag for Bovine Serum Albumin Detection

Abstract

The availability of rapid-portable instruments to monitor the bovine serum albumin (BSA) concentration is vital in the early detection of disease. In this research, an original biosensing method based on a spin-valve giant magnetoresistance as a transducer and magnetite@silver core-shell magnetic nanoparticles (MNPs) as a tag has been proposed for detecting BSA. The synthesized magnetite@silver MNPs tag size is ≈15 nm with ≈52 emu g −1 of saturation magnetization. This proposed biosensor performs rapid detection of BSA through the shifting of the switching magnetic field and the increasing of the output voltage. The effectivity of the sensor in monitoring the BSA concentration was shown by a strong linear correlation with ≈0.06 mV/(mg/ml) of the sensitivity and ≈0.44 mg ml −1 of the detection limit. Moreover, the measurement result can be acquired rapidly up to 1 min with low external magnetic field assistance. Therefore, this biosensing technique can be promoted as a real-time portable biosensor.

A New Platform of Iron Oxide-Based Nanoparticles Assay Using GMR Chip-Based Sensor With Microcontroller

“…Successively, the surface of Fe 3 O 4 was modified using (3-aminopropyl)trimethoxysilane to facilitate the Ag encapsulation. The synthesizing procedure of core-shell Fe 3 O 4 @Ag has been reported in detail in the previous work [19]. The core-shell Fe 3 O 4 @Ag MNPs to be detected in this work are mostly spherical with (15 ± 2.6) nm of average size [Fig.…”

“…Our previous works [19], [20] have demonstrated the effective usage of the surface-functionalized MNPs, i.e., core-shell Fe 3 O 4 @Ag, as the magnetic label for GMR-based biosensors. These MNPs not only have sufficient magnetization so their concentration can be quantified using GMR sensing elements but also can bind the bovine serum albumin (BSA) spontaneously [13], [21].…”

Review—Potential of Tunneling Magnetoresistance Coupled to Iron Oxide Nanoparticles as a Novel Transducer for Biosensors-on-Chip

“…Numerous studies have been undertaken on this topic. 41,51,85,87,88 Wibowo, et al proposed the quantification method of silver-coated magnetite using a GMR chip manufactured by NVE Corp. 85 By using a similar chip, Ardiyanti, et al perform the successful quantification of GSmagnetite and streptavidin-coated MNPs. 41 Each research reveals the linear dependence of the sensor signal to the MNPs amount with the sensitivity in the order of mV/mg/mL.…”

“…Using the spin-valve GMR bulk thin film, Wibowo, et al showed the successful quantification of Fe 3 O 4 @Ag MNPs 88 as well as Fe 3 O 4 @Ag-labeled serum albumin. 87 By using an analogous GMR sensing element, Mabarroh, et al reported the detection of GS-Fe 3 O 4 as well as GS-Fe 3 O 4 labeled α-amylase enzyme. 51 The successful usage of the GMR sensor for bio-detection shows the potential usage of the TMR sensor, which has a better MR ratio than GMR thereby consuming less energy, 89 for the forthcoming BoC field.…”