A “spiral test” applied to bacterial mutagenesis assays

Flora, Silvio De

doi:10.1016/0027-5107(81)90151-2

Cited by 13 publications

(2 citation statements)

References 12 publications

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“…Forward mutation systems have also been proposed that have the advantage that only a single strain of the bacterium needs to be tested [Miller et al, ]. The second group of screens employ the standard strains listed by OECD but use a different format than the standard plate incorporation and pre‐incubation methods. These include systems where a concentration gradient of the test compound is generated across an agar plate, e.g., the spot test [Maron and Ames ], a wedge/slope method [McMahon et al, ] and the spiral plate method [de Flora , Diehl and Fort , Claxton et al, ]. A modified pre‐incubation method can be used to detect the mutagenicity of chemicals in limited supply; in this case small volumes of the test solution are pre‐incubated with a concentrated suspension of the bacteria before plating out using the standard volumes of top and bottom agar [Kado et al, ].…”

Section: Introductionmentioning

confidence: 99%

The micro‐Ames test: A direct comparison of the performance and sensitivities of the standard and 24‐well plate versions of the bacterial mutation test

Proudlock¹,

Evans²

2016

Environ and Mol Mutagen

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"Ames" bacterial mutation tests are widely performed for evaluation and registration of new materials including industrial chemicals, agrochemicals, medical devices, pharmaceuticals, pharmaceutical impurities and other materials. Tests are used to predict their potential long-term adverse health effects (including carcinogenicity). Given their importance, pre-screening 'miniaturized' versions have been developed which allow higher throughput and use less test material, including the widely-employed 24-well micro-Ames (µAmes) test which uses 20 times less material. However, little quantitative information has been published on the methodology or sensitivity of this system. We describe methods and results used in direct comparisons of the sensitivity of micro and standard systems using the same cultures, formulations, etc. Initial testing utilized the plate incorporation method and, later, the pre-incubation method. In a subsequent phase of testing, a four-way direct comparison was made between the pre-incubation and plate incorporation methods in both systems using some direct-acting mutagens. Tests used only those strain/S9/chemical combinations where a response was expected. Historical control results accumulated during testing are also presented. Spontaneous and induced revertant colony counts for the µAmes system were consistently proportionate and approximately 1/20th those for the standard Ames test. Sensitivities of the two systems were found to be nearly identical in almost all cases for a wide variety of weak and strong inorganic and organic mutagens. Standardized procedures and increased reliability of the estimate of the background revertant frequency in the µAmes system means that the two systems give equivalent results and are expected to be highly predictive of one another. Environ. Mol. Mutagen. 57:687-705, 2016. © 2016 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

The micro‐Ames test: A direct comparison of the performance and sensitivities of the standard and 24‐well plate versions of the bacterial mutation test

Proudlock¹,

Evans²

2016

Environ and Mol Mutagen

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“…After a 48-hr incubation, the number of colonies on each petri plate is counted to determine if the test substance induced an increase in the number of mutant colonies compared to vehicle controls. Recently, an automated spiral Salmonella mutagenicity assay was introduced which simplifies testing and reduces the amount of test substance needed to conduct the assay [de Flora, 1981;Houk et al, 19891. Previous validation studies of the spiral assay have found it to have good agreement with the standard pour-plate assay Mayo and Aaron, 19911, but with increased sensitivity in terms of the dose-response obtained [Houk et al, 1989.…”

Section: Introductionmentioning

confidence: 99%

Spiral Salmonella assay: Validation against the standard pour-plate assay

Diehl

Fort

1996

Environ. Mol. Mutagen.

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Trimethylphosphat [MAK Value Documentation in German language, 1983]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 9. Lieferung, Ausgabe 1983 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Metabolismus und Ausscheidung von TMP Erfahrungen beim Menschen Tierexperimentelle Befunde Zur Frage eines MAK‐Wertes

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A “spiral test” applied to bacterial mutagenesis assays

Cited by 13 publications

References 12 publications

The micro‐Ames test: A direct comparison of the performance and sensitivities of the standard and 24‐well plate versions of the bacterial mutation test

The micro‐Ames test: A direct comparison of the performance and sensitivities of the standard and 24‐well plate versions of the bacterial mutation test

Spiral Salmonella assay: Validation against the standard pour-plate assay

Trimethylphosphat [MAK Value Documentation in German language, 1983]

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