To determine the association between functional screening tests and lower body, non-contact injuries in Division I women basketball, soccer, and volleyball student-athletes (SA). 68 injury-free women SA (age:19.1±1.1 yrs, height:171.3±8.7 cm, mass:68.4± 9.5 kg) were tested preseason with single (SH), triple (TH), and crossover (XH) hop for distance, and isometric hip strength (abduction, extension, and external rotation) in randomized order. The first lower body (spine and lower extremity), non-contact injury requiring intervention by the athletic trainer was abstracted from the electronic medical record. Receiver operating characteristic and area under the curve (AUC) were calculated to determine cut-points for each hopping test from the absolute value of between limb difference. Body weight-adjusted strength was categorized into tertiles. Logistic regression determined the odds of injury with each functional screening test using the hopping tests cut-points and strength categories, adjusting for previous injury history. 52 SA were injured during the sport season. The cut-point for SH was 4 cm (sensitivity=0.77, specificity=0.43, AUC=0.53), and TH and XH was 12 cm (sensitivity=0.75 and 0.67, specificity=0.71 and 0.57, AUC=0.59 and 0.41, respectively). A statistically significant association with TH and injuries (adjusted odds ratio=6.50 [95% confidence interval:1.69-25.04]) was found. No significant overall association was found with SH or XH, nor with the strength tests.Using a clinically relevant injury definition, the TH showed the strongest predictive ability for non-contact injuries. This hopping test may be a clinically useful tool to help identify increased risk of injury in women SA participating in high-risk sports.