2022
DOI: 10.1101/2022.05.09.491210
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A sporulation-independent way of life for Bacillus thuringiensis in the late stages of an infection

Abstract: The formation of endospores has been considered as the unique mode of survival and transmission of sporulating Firmicutes due to the exceptional resistance and persistence of this bacterial form. However, the persistence of non-sporulated bacteria (Spo-) was reported during infection in Bacillus thuringiensis, an entomopathogenic sporulating Gram-positive bacterium. In this study, we investigated the behavior of a bacterial population in the late stages of an infection as well as the characteristics of the Spo… Show more

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“…To compare the sporulation capacity of B. thuringiensis and its variants, a sporulation assay was performed similarly to the description by Toukabri et al [37]. Firstly, overnight cultures (24 h) of the ancestor and variants were serially diluted and plated on TSB Congo Red to quantify the concentration vegetative cells in each culture.…”
Section: Sporulation Assaymentioning
confidence: 99%
“…To compare the sporulation capacity of B. thuringiensis and its variants, a sporulation assay was performed similarly to the description by Toukabri et al [37]. Firstly, overnight cultures (24 h) of the ancestor and variants were serially diluted and plated on TSB Congo Red to quantify the concentration vegetative cells in each culture.…”
Section: Sporulation Assaymentioning
confidence: 99%