A Spot Segmentation Approach for 2D Gel Electrophoresis Images Based on 2D Histograms

Zacharia, Eleni; Kostopoulou, Eirini; Maroulis, Dimitris; Κοσσίδα, Σοφία

doi:10.1109/icpr.2010.622

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Pattern recognition of 2D PCR-RFLP images had been used for lane intensity profile by removing background intensity (Maramis and Delopoulos, 2010). Pattern recognition of 2D Images is being extensively used discrimination of Plants, 2D fingerprints, Spot segmentation of 2D histograms etc., (Rodriguez et al, 2014, Ye et al, 2011, Zhai et al, 2010, Zacharia et al, 2010). HIBS also uses Pattern recognition for band detection, but this software has ability to compare Low range DNA ladder 100 bp (Fermentas cat# SMO 323) and PCR product and auto recognize the size of every primer amplification and number of amplifications.…”

Section: Resultsmentioning

confidence: 99%

Forensic STR profiling based smart barcode, a highly efficient and cost effective human identification system

Zahra

Hussain

Jamil

et al. 2018

Saudi Journal of Biological Sciences

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In forensic science, the human identification is the major goal in criminal cases and in paternity dispute, based on DNA profiling of individual. Analysis of short tandem repeat (STRs) markers used as a reliable technique for this purpose. In this study the main objective was to develop a human identification based on DNA fingerprinting using Human Identification Barcode System (HIBS). HIBS may be used to provide a unique molecular signature of human in the form of a barcode. DNA was isolated from blood by using PCR technique to detect bands to design human barcode using self-designed system. HIBS is a web based application that can be accessed via www.hibs.com.pk. HIBS can be accessed with internet access and may be introduced on security checkpoints to identify an individual based on his own DNA instead of conventional procedures of identification. The barcode generated through DNA fingerprinting will be stored in a HIBS, and may also be a part of CNIC. It may be successfully used against suicide bombers, target killers, etc., as even a single blood spot, a few skin cells, root of the hair etc., to identify such culprits. It may also be effectively used to relieve the individuals with false accusations.

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Section: Resultsmentioning

confidence: 99%

Forensic STR profiling based smart barcode, a highly efficient and cost effective human identification system

Zahra

Hussain

Jamil

et al. 2018

Saudi Journal of Biological Sciences

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Cytoplasm and nucleus segmentation in cervical smear images using Radiating GVF Snake

Wenyin

et al. 2012

Pattern Recognition

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130

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An effective approach for detection and segmentation of protein spots on 2-d gel images

Kostopoulou

Zacharia

Maroulis

2014

IEEE J. Biomed. Health Inform.

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Two-dimensional gel image analysis is widely recognized as a particularly challenging and arduous process in proteomics field. The detection and segmentation of protein spots are two significant stages of this process as they can considerably affect the final biological conclusions of a proteomic experiment. The available techniques and commercial software packages deal with the existing challenges of 2-D gel images in a different degree of success. Furthermore, they require extensive human intervention which not only limits the throughput but unavoidably questions the objectivity and reproducibility of results. This paper introduces a novel approach for the detection and segmentation of protein spots on 2-D gel images. The proposed approach is based on 2-D image histograms as well as on 3-D spots morphology. It is automatic and capable to deal with the most common deficiencies of existing software programs and techniques in an effective manner. Experimental evaluation includes tests on several real and synthetic 2-D gel images produced by different technology setups, containing a total of ∼ 21,400 spots. Furthermore, the proposed approach has been compared with two commercial software packages as well as with two state-of-the-art techniques. Results have demonstrated the effectiveness of the proposed approach and its superiority against compared software packages and techniques.

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A Spot Segmentation Approach for 2D Gel Electrophoresis Images Based on 2D Histograms

Cited by 3 publications

References 11 publications

Forensic STR profiling based smart barcode, a highly efficient and cost effective human identification system

Forensic STR profiling based smart barcode, a highly efficient and cost effective human identification system

Cytoplasm and nucleus segmentation in cervical smear images using Radiating GVF Snake

An effective approach for detection and segmentation of protein spots on 2-d gel images

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