A Stability-Indicating and Validated Reverse-Phase High-Performance Liquid Chromatographic Method for the Simultaneous Estimation of Phenylephrine  and Fexofenadine in Bulk and Tablet Dosage Forms

Kumar, B.sai; Jyothi, G.; B, Sreenivasa Rao

doi:10.22159/ajpcr.2016.v9s2.13371

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…It is widely used for treatment of respiratory infections (bronchitis, pneumonia) and urinary infections. A few analytical techniques have been constructed for the quantification of Gemifloxacin in pure forms, such as, Spectrophotometric method [25][26] , HPTLC 27 , RP-HPLC [28][29] , LC-MS 30 , UV-Spectrofluorometric method 31 and Zone electrophoresis 32 .…”

Section: Gemifloxacin Chemically Is [{Rs}7-[{4z}-3-{aminomethyl}-mentioning

confidence: 99%

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2019

IJPSR

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Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Cetirizine hydrochloride, Duloxetine hydrochloride, Esmolol hydrochloride, Gemifloxacin, Moxifloxacin and Phenylephrine Hydrochloride based on their reactivity towards Chloramine-T (ChL-T). The method is based on the oxidation of drugs by ChL-T (Excess amount) in presence of acidic medium and estimating the amount of un-reacted ChL-T by Safranin-O (SO) dye at λmax 524 nm. The amount of ChL-T reacted is equal to the drug concentration in this method. The concentration of ChL-T is 10 µgmL-1 and Safranin-O Dye is 10 µgmL-1. The calibration curves obeyed Beer's law over the concentration range of 2.5-17.5 µgmL-1 (CET), 5-35 µgmL-1 (DUL), 12-84 µgmL-1 (ESM), 10-70 µgmL-1 (GEM) & 2.5-17.5 µgmL-1 (MOX). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of International Conference on Harmonization. INTRODUCTION: Cetirizine dihydrochloride chemically is [2-[4-[(4-chlorophenyl) phenyl methyl]-1 piperazinyl] ethoxy] acetic acid di HCl. Cetirizine di HCl is belonged to the group of the second-generation h1 antagonist, inhibits allergic reaction mediated by histamine.

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Section: Gemifloxacin Chemically Is [{Rs}7-[{4z}-3-{aminomethyl}-mentioning

confidence: 99%

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2019

IJPSR

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“…Literatürde atorvasatin ve ezetimib etken maddenin farklı analitik yöntemlerle analizleri mevcuttur. Ezetimib etken maddesinin atorvasatin etken maddesinden farklı ilaç grupları ile ters faz yüksek basınçlı sıvı kromatografi yöntemi ile [4,5,6,7], sıvı kromatogarfi-kütle/kütle spektrometri ile (LC-MS/MS) [8], voltametrik yöntemle [9], spektrofotometrik yöntemle [10]; atorvastatin etken maddesinin yüksek performans ince tabaka metodu ile [11], spektrofotometrik metotla [12,13] analizleri yapılmıştır.…”

Section: Introductionunclassified

Kolestrolü Tedavi Edici İlaç Kombinasyonlarının Eşzamanlı Spektrofotometrik Tayini

Pekcan,

Bakan

2024

Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Bu çalışmada, kolestrol yüksekliği tedavisinde kullanılan ilaç etken maddelerinde atorvasatin ve ezetimibin miktar tayinleri kemometrik programlardan olan kısmi en küçük kareler metodu ile ultra viyole görünür alan spektroskopisi verileri öz önüne alınarak hesaplanmıştır. Kısmi en küçük kareler (PLS) yöntemi ile hesaplanan değerlere bakıldığında geri kazanım değerleri oldukça yüksek, bağıl standart sapma değerleri de yeterince küçük değerler olarak elde edilmiştir. Gerçek ve tahmini yani hesaplanan değerler arasındaki ilişkide de regresyon katsayıları bir değerine yakın bulunmuştur. Yöntemin geçerliliği için analitik parametreler hesaplanmıştır. Ticari ilaç tabletlerine geçmeden önce uygulanan yöntemlerin uygunluğu da kontrol edilmiştir. Bu işlem için ANOVA testi yapılmıştır. F hesaplanan değerleri F ölçüt ya da F teorik değerlerinden düşük olmalıdır. P değeri olan pearson korelasyon katsayısının da 0,05 değerinden büyük olması gerekmektedir. Hesaplanan değerler ile yöntemin uygun olduğuna karar verilmiştir. Bu çalışmada uygulanan yöntemler, iki bileşenli karmaşık ilaç karışımlarına uygulanabilmektedir.

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Validated Rp-HPLC and Uv-Spectroscopy Methods for the Estimation of Dapagliflozin in Bulk and in Tablets

Sabbagh¹,

Lokesh²,

Akouwah³

2017

IND. DRU.

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Two methods were developed for the determination of dapagliflozin (DAPA) in pure form and in tablets. The procedure utilized was UV-Visible Spectroscopy and RP-HPLC with PDA detector to quantify DAPA in bulk and tablets. The sensitive linear range was identified for both methods within 0.5-5.0μg/mL. The linear regression analysis was identified for both methods with correlation coefficient(r)>0.99. The LOD and LOQ values were found to be 0.05 μg/mL and 0.5 μg/mL for the method by UV-Spectroscopy. The molar absorptivity (ε) was calculated as 1.27 X 105 L.mol-1cm-1. The RP-HPLC method produced LOD and LOQ values of 1.0 ng/mL and 0.5 μg/mL. Both methods were simple, precise, reproducible to quantify the amount of unknown in bulk as well as in tablets and estimated accurately within the range of 100.0±0.5%. Statistical analysis was performed on the data obtained. There was no significant difference between the developed and reported methods with p>0.05. Both methods can be applied for routine analysis of DAPA in bulk and tablets with good accuracy and precision.

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A Stability-Indicating and Validated Reverse-Phase High-Performance Liquid Chromatographic Method for the Simultaneous Estimation of Phenylephrine and Fexofenadine in Bulk and Tablet Dosage Forms

Cited by 3 publications

References 3 publications

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Kolestrolü Tedavi Edici İlaç Kombinasyonlarının Eşzamanlı Spektrofotometrik Tayini

Validated Rp-HPLC and Uv-Spectroscopy Methods for the Estimation of Dapagliflozin in Bulk and in Tablets

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