A stability-indicating HPLC-UV method for the quantification of anthocyanin in Roselle (Hibiscus Sabdariffa L.) spray-dried extract, oral powder, and lozenges

Khan, Nasir Hayat; Abdulbaqi, Ibrahim M.; Darwis, Yusrida; Aminu, Nafiu; Chan, Siok-Yee

doi:10.1016/j.heliyon.2022.e09177

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…In the present work conditions, 58% of the anthocyanin incorporated through chilto juice into the beverage was recovered after drying. These results showed that anthocyanins are less stable under drying conditions with a high temperature as reported in other studies on degradation of anthocyanin by temperature [70,71]. FTIR spectra of freeze-dried powders are shown in Figure 4.…”

Section: Beverage Formulation and Chemical Characterizationsupporting

confidence: 82%

Powdered Beverage from Native Plants from Argentina (Zuccagnia punctata and Solanum betaceum) Obtained by Spray-Drying: A Promising Source of Antioxidant Compounds

Uriburu

Zampini

Maldonado

et al. 2023

Plants

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In previous studies, the Argentinean native plants called Zuccagnia punctata (jarilla, pus pus, lata) and Solanum betaceum (chilto, tree tomato) were reported as new natural sources of antioxidant compounds, mainly chalcones, anthocyanins and rosmarinic acid derivates. The present study deals with the production of antioxidant beverages of Z. punctata (Zp) extract and chilto juice with honey as sweetener. A Zp extract and red chilto juice were obtained according to Food Code and characterized. The beverages were formulated by using maltodextrin (MD) with two dextrose equivalents (DE), 10 and 15, and then spray-dried at an inlet air temperature of 130 °C. The physicochemical, microscopical, phytochemical and functional characteristics of the powders were surveyed. The experiments carried out showed good physical properties for both formulations showing high water solubility with adequate features for handling, transport and storage. The chromatic parameters of both powdered beverages indicate orange–pink tones regardless of the wall material used. The total polyphenol and flavonoid content in the beverages were kept after spray-drying (92 and 100%, respectively). The anthocyanins were less stable under drying conditions (yield 58%). Both powdered beverages showed high scavenger capacity on ABTS•+, HO• and H2O2 (SC50 between 3.29 to 41.05 µg GAE/mL) and were able to inhibit xanthine oxidase (XOD) activity (CI50 between 91.35 and 114.43 µg GAE/mL). The beverages were neither toxic nor mutagenic in the concentration range with biological activity. The results obtained in the present work scientifically support the use of the powdered beverages of Argentinean native plants as antioxidant.

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Section: Beverage Formulation and Chemical Characterizationsupporting

confidence: 82%

Powdered Beverage from Native Plants from Argentina (Zuccagnia punctata and Solanum betaceum) Obtained by Spray-Drying: A Promising Source of Antioxidant Compounds

Uriburu

Zampini

Maldonado

et al. 2023

Plants

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“…One of the primary challenges in quality control is identifying and evaluating the sources of variability in the chemical composition of these raw materials. Stability is a significant factor that can influence chemical quality of raw materials, and stability‐indicating methods can be used to control this factor (Gomes et al, 2018; Khan et al, 2022; Ratnatilaka Na Bhuket et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Validation of stability‐indicating LC method, degradation study, and impact on antioxidant and antifungal activities of Eugenia uniflora leaves extract

Santos

Lima

Machado

et al. 2023

Biomedical Chromatography

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The aim of this study is to demonstrate the stability‐indicating capacity of an analytical method for Eugenia uniflora, enhance understanding of the stability of myricitrin, and assess the effect of degradation of spray‐dried extract (SDE) on antioxidant and antifungal activities. Validation of the stability‐indicating method was carried out through a forced degradation study of SDE and standard myricitrin. The antioxidant and antifungal activities of SDE were evaluated both before and after degradation. The quantification method described was found to be both accurate and precise in measuring myricitrin levels in SDE from E. uniflora, with excellent selectivity that confirmed its stability‐indicating capability. The forced degradation study revealed that the marker myricitrin is sensitive to hydrolysis, but generally stable under other stress conditions. By contrast, the standard myricitrin displayed greater susceptibility to degradation under forced degradation conditions. Analysis of the antioxidant activity of SDE before and after degradation showed a negative impact in this activity due to degradation, while no significant effect was observed on antifungal activity. The method described can be a valuable tool in the quality control of E. uniflora, and the findings can assist in determining the optimal conditions and storage of products derived from this species.

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“…The results were expounded by calculating the RSD%. In precision studies, the standard deviation of results should be less or equal to 2% [52]. Recovery % was detected in the range of 99.273‐101.032% and 99.017‐101.983% in the intra‐day and inter‐day analysis, respectively.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical profiling, in vitro biological activities, and in silico (molecular docking and absorption, distribution, metabolism, excretion, toxicity) studies of Polygonum cognatum Meissn

Akpınar,

Yıldırım Baştemur,

Bıçak

et al. 2023

J of Separation Science

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Polygonum cognatum Meissn, a perennial herbaceous belonging to the Polygonaceae family, is an aromatic plant. High‐performance liquid chromatography/diode array detector method was developed and validated for the phytochemical analysis of the plant. Also, various methods were used to investigate the antioxidant, antimicrobial, and cytotoxic activities of the methanolic extracts. Antioxidant activities were researched by 2,2′‐diphenyl‐1‐picrylhydrazyl and cupric reducing antioxidant capacity methods. Among the tested standard microbial strains, Candida albicans was found to be more sensitive with a 24.60 ± 0.55 mm inhibition zone according to the diffusion tests. In the microdilution tests, the minimum inhibitory concentration and minimum bactericidal/fungicidal concentration values were 4.75 and ≥ 4.75 mg/mL, respectively, for all tested pathogens. Human colon carcinoma cells were used to investigate cytotoxicity by using 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide analysis (IC50 = 2891 μg/mL for Plant A, IC50 = 3291 μg/mL for Plant B). Molecular docking and absorption, distribution, metabolism, excretion, and toxicity analysis were used to explain inhibition mechanisms of major phenolic compounds of plants against Tankyrase 1, Tankyrase 2 enzymes, and deoxyribonucleic acid gyrase subunit B and found compatible with experimental results.

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A stability-indicating HPLC-UV method for the quantification of anthocyanin in Roselle (Hibiscus Sabdariffa L.) spray-dried extract, oral powder, and lozenges

Cited by 10 publications

References 34 publications

Powdered Beverage from Native Plants from Argentina (Zuccagnia punctata and Solanum betaceum) Obtained by Spray-Drying: A Promising Source of Antioxidant Compounds

Powdered Beverage from Native Plants from Argentina (Zuccagnia punctata and Solanum betaceum) Obtained by Spray-Drying: A Promising Source of Antioxidant Compounds

Validation of stability‐indicating LC method, degradation study, and impact on antioxidant and antifungal activities of Eugenia uniflora leaves extract

Phytochemical profiling, in vitro biological activities, and in silico (molecular docking and absorption, distribution, metabolism, excretion, toxicity) studies of Polygonum cognatum Meissn

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