A stable sheep cell preparation for detecting thyroglobulin auto-antibodies and its clinical applications

Abstract

SYNOPSISThe preparation of stable formolized sheep cells coated with thyroglobulin and their use for the detection of auto-antibodies in human thyroiditis is described. This provides an easy laboratory test which gives results closely comparable with those using fresh tanned cells.The agglutination titres do not closely parallel the antibody content of the serum but a good correlation may be obtained by inhibiting agglutination with thyroglobulin.The diagnostic usefulness of the test in treating disorders of the thyroid is discussed and related to the results of tests for the other two immune systems involved in thyroiditis. The tanned red cell test may be used to differentiate Hashimoto's disease from thyroid cancer and non-toxic colloid goitre, and to assess the choice of treatment in patients with Graves' disease. It has proved of particular value in genetic studies of auto-immunity.

Suppression of Thyroid Function during Ingestion of Seaweed "Kombu" (Laminaria japonoca) in Normal Japanese Adults

“…Anti-thyroid antibodies, anti-thyroglobulin and -microsome antibodies, were determined by the agglutination method (thyroid and microsome tests) [7,8] using commercial kits of Serodia-ATG R and -AMC R (Fuji Rebio Co. Ltd.). On each urination, part of urine was collected at a constant ratio (1/50-5/50) using Urine-mate R (Sumitomo Bakelite Co. Ltd.).…”

Cytotoxic T lymphocyte antigen-4 (CTLA-4) gene polymorphisms and susceptibility to type 1 autoimmune hepatitis

“…The latter individual was retained in the analysis because she also had ANA. 23 Thyroglobulin and microsomal thyroid antibodies were sought in 152 patients (98%) by standard techniques [24][25][26] and a titer of at least 1:100 was considered positive.…”

Evaluation of a tanned red cell technique for thyroid microsomal antibodies

“…Many workers with the TRC test use serum dilutions of 1:5, 1:10, and then 10-fold dilutions up to 1:2 500 000 or more, according to the method of Fulthorpe et al (1961). We It also produced a reduction in the titre of thyroglobulin antibody which might be due to the presence of thyroglobulin in the antigen.…”