A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes

Scheu, Max; Komorowski, Karlo; Shen, Chen; Salditt, Tim

doi:10.1007/s00249-020-01493-2

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…For example, on one hand Yang and Huang ( 2002 , 2003 ) were able to directly visualize a “static” stalk formation in dehydrating lipid membrane stacks. On the other hand, Salditt and colleagues captured transient “dynamic” stalk formations, presenting them as structural fusion assays, in aligned multi-lamellar stacks of protein-free-lipid membranes (Aeffner et al 2012 ; Scheu et al 2021 ).…”

Section: From Advent Of Liposomes To Development Of Experimental Assa...mentioning

confidence: 99%

Aspects of Biological Replication and Evolution Independent of the Central Dogma: Insights from Protein-Free Vesicular Transformations and Protein-Mediated Membrane Remodeling

Mittal

Chauhan

2022

J Membrane Biol

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Biological membrane remodeling is central to living systems. In spite of serving as “containers” of whole-living systems and functioning as dynamic compartments within living systems, biological membranes still find a “blue collar” treatment compared to the “white collar” nucleic acids and proteins in biology. This may be attributable to the fact that scientific literature on biological membrane remodeling is only 50 years old compared to ~ 150 years of literature on proteins and a little less than 100 years on nucleic acids. However, recently, evidence for symbiotic origins of eukaryotic cells from data only on biological membranes was reported. This, coupled with appreciation of reproducible amphiphilic self-assemblies in aqueous environments (mimicking replication), has already initiated discussions on origins of life beyond nucleic acids and proteins. This work presents a comprehensive compilation and meta-analyses of data on self-assembly and vesicular transformations in biological membranes—starting from model membranes to establishment of Influenza Hemagglutinin-mediated membrane fusion as a prototypical remodeling system to a thorough comparison between enveloped mammalian viruses and cellular vesicles. We show that viral membrane fusion proteins, in addition to obeying “stoichiometry-driven protein folding”, have tighter compositional constraints on their amino acid occurrences than general-structured proteins, regardless of type/class. From the perspective of vesicular assemblies and biological membrane remodeling (with and without proteins) we find that cellular vesicles are quite different from viruses. Finally, we propose that in addition to pre-existing thermodynamic frameworks, kinetic considerations in de novo formation of metastable membrane structures with available “third-party” constituents (including proteins) were not only crucial for origins of life but also continue to offer morphological replication and/or functional mechanisms in modern life forms, independent of the central dogma. Graphic Abstract

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Section: From Advent Of Liposomes To Development Of Experimental Assa...mentioning

confidence: 99%

Aspects of Biological Replication and Evolution Independent of the Central Dogma: Insights from Protein-Free Vesicular Transformations and Protein-Mediated Membrane Remodeling

Mittal

Chauhan

2022

J Membrane Biol

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“…Typical approaches in membrane studies use bilayer systems such as liposomes (Zhang et al, 1994;Heftberger et al, 2014;Konarev et al, 2021;Kucerka, Tristram-Nagle et al, 2005), hydrated supported multilayers (Kucerka, Liu et al, 2005;Nagle, Cognet et al, 2019;Scheu et al, 2021;Kucerka, Tristram-Nagle et al, 2005;Hristova & White, 1998), solid supported bilayers at bulk interfaces (Koutsioubas et al, 2017;de Ghellinck et al, 2015), or monolayers at air-water or oilwater interfaces (Stefaniu et al, 2014). These models depict representative features of interest of more complex biological membranes, allowing high-resolution structures to be acquired by applying suitable X-ray or neutron scattering methods (Zhang et al, 1994;Lyatskaya et al, 2000;Heftberger et al, 2014;Stefaniu et al, 2014;Koutsioubas et al, 2017;Konarev et al, 2021;Kucerka, Tristram-Nagle et al, 2005;Hristova & White, 1998).…”

Section: Introductionmentioning

confidence: 99%

Absolute scattering length density profile of liposome bilayers obtained by SAXS combined with GIXOS: a tool to determine model biomembrane structure

Harvey,

Bello,

Kikhney

et al. 2023

J Appl Cryst

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Lipid membranes play an essential role in biology, acting as host matrices for biomolecules like proteins and facilitating their functions. Their structures and structural responses to physiologically relevant interactions (i.e. with membrane proteins) provide key information for understanding biophysical mechanisms. Hence, there is a crucial need of methods to understand the effects of membrane host molecules on the lipid bilayer structure. Here, a purely experimental method is presented for obtaining the absolute scattering length density profile and the area per lipid of liposomal bilayers, by aiding the analysis of small-angle X-ray scattering (SAXS) data with the volume of bare headgroups obtained from grazing-incidence X-ray off-specular scattering (GIXOS) data of monolayers of the same model membrane lipid composition. The GIXOS data experimentally demonstrate that the variation of the bare headgroup volume upon change in lipid packing density is small enough to allow its usage as a reference value without knowing the lipid packing stage in a bilayer. This approach also has the advantage that the reference volume is obtained in the same aqueous environment as used for the model membrane bilayers. The validity of this method is demonstrated using several typical membrane compositions, as well as one example of a phospholipid membrane with an incorporated transmembrane peptide. This methodology allows us to obtain absolute scale rather than relative scale values using solely X-ray-based instrumentation, retaining a similar resolution to SAXS experiments. The method presented has high potential for understanding the structural effects of membrane proteins on the biomembrane structure.

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“…Typical approaches in membrane studies use bilayer systems such as liposomes 2,[5][6][7] , hydrated supported multilayers [7][8][9][10] , solid supported bilayers at bulk interfaces 11,12 , or monolayers at air-water or oil-water interfaces 4 . These models depict representative features of interest of more complex biological membranes, allowing high resolution structures to be acquired by applying suitable Xray or neutron scattering methods 2,[4][5][6][7]11,13 .…”

Section: Introductionmentioning

confidence: 99%

Absolute scattering length density profile of liposome bilayers obtained by SAXS combined with GIXOS - a tool to determine model biomembrane structure

Shen

Wölk

Kikhney

et al. 2022

Preprint

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Lipid membranes play an essential role in biology, acting as host matrices for biomolecules like proteins and facilitating their functions. Their structures, and structural responses to physiologically relevant interactions, i.e. with membrane proteins, provide key information for understanding biophysical mechanisms. Hence, there is a crucial need of methods to understand the effects of membrane host molecules on the lipid bilayer structure. Here, we present a purely experimental method for obtaining the absolute scattering length density (SLD) profile and the area per lipid of liposomal bilayers, by aiding the analysis of small angle X-ray scattering (SAXS) data with the volume of bare headgroups obtained from fast (20-120s) grazing incidence off-specular scattering (GIXOS) data from monolayers of the same model membrane lipid composition. The GIXOS data experimentally demonstrate that the variation of the bare headgroup volume upon lipid packing density change is small enough to allow its usage as a reference value without knowing the lipid packing stage in a bilayer. This approach also bares the advantage that the reference volume is obtained at the same aqueous environment as used for the model membrane bilayers. We demonstrate the validity of this method using several typical membrane compositions, as well as one example of a phospholipid membrane with an incorporated transmembrane peptide. This methodology allows to obtain absolute scale values rather than relative scale by using solely X-ray-based instrumentation, retaining a similar resolution of SAXS experiments. The presented method has high potential to understand structural effects of membrane proteins on the biomembrane structure.

show abstract

A stalk fluid forming above the transition from the lamellar to the rhombohedral phase of lipid membranes

Cited by 5 publications

References 41 publications

Aspects of Biological Replication and Evolution Independent of the Central Dogma: Insights from Protein-Free Vesicular Transformations and Protein-Mediated Membrane Remodeling

Aspects of Biological Replication and Evolution Independent of the Central Dogma: Insights from Protein-Free Vesicular Transformations and Protein-Mediated Membrane Remodeling

Absolute scattering length density profile of liposome bilayers obtained by SAXS combined with GIXOS: a tool to determine model biomembrane structure

Absolute scattering length density profile of liposome bilayers obtained by SAXS combined with GIXOS - a tool to determine model biomembrane structure

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