A standard experimental ‘chemical burn’

Kim, J.; Weibel, T.J.; Carter, E.J.; Calobrace, M.B.; Foldi, J.F.; Zawacki, Bruce E.

doi:10.1016/0305-4179(94)90182-1

Cited by 5 publications

(2 citation statements)

References 6 publications

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“…An apparatus to produce a standardized contact burn was developed decades ago, 5 and until recently, different models of full-thickness contact burns with differently sized and heated brass bars were described. 6Y8 Kim et al 9 published a standardized experimental ''chemical burn'' based on the Walker-Mason model.…”

Section: Discussionmentioning

confidence: 99%

Innovative scald burn model and long-term dressing protector for studies in rats

Pfurtscheller

Petnehazy²,

Goessler³

et al. 2013

Journal of Trauma and Acute Care Surgery

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R ats are frequently used for experimental studies in burns.Scalding is the easiest mechanism for provoking a dermal burn; the ability to vary water temperature, time of exposure, and the burned area make this method effective for reproducing almost any kind of thermal wound. Last but not least, rats are readily available, inexpensive, and easy to manage.In general, a scald injury in rats is inflicted on anesthetized animals by immersion of the area to be burned into a water bath. The area of exposure is determined by the aperture in the template, which can be an elaborately designed apparatus, 1 a metal wire cage, 2 or a simple metal plate with a rectangular aperture 3 through which the body part is immersed in a water bath for a specified amount of time.To establish a standard long-term method for protecting the burn wound dressings from being eaten by the rats and thereby tainting the scientific results, we searched the relevant literature and found that there was nothing available for that purpose. After several tests, the armor described later used together with our scald template was found to be satisfactory for our experiment series. With the use of the armor, the silver detected in the blood should originate only from the wound, with no additional absorption from the gut.In this article, an innovative scald protocol and armor are being presented. With this template firmly pressed on the shaved back, only the area to be burned is in contact with the boiling water. The burn wound is uniform in size and depth and can certainly be reproduced. The template is easy and fast to fabricate at a low cost and at any size. The armor ensures that the dressings stay in place for a minimum of four or even more weeks and prevents the rats from eating the silver-coated wound material. MATERIALS AND METHODS Experimental Scald ModelTen female and 4 male Sprague-Dawley rats (Himberg, Austria) weighing 250 g to 350 g were allowed to acclimatize for 1 week before use. The animals had free access to water and standard rat chow with intermittent cycles of light and dark in a temperature-controlled environment at The experiments were performed in accordance with European and Austrian laws on animal experimentation. All animals were weighed and then preanaesthetized in an anesthesia-induction box with an isoflurane-oxygen mix (0Y4% isoflurane vaporizer setting in 100% oxygen, stepwise increase; oxygen flow, 1.5 L/min.). Once asleep, midazolam (2 mg/kg), medetomidine (0.15 mg/kg) and fentanyl (5 Kg/kg) were administered subcutaneously, and the eyes were protected with oleovit-A eye ointment. The full anesthetic effect was achieved after 5 minutes, and the scalding procedure was started when there was no more toe pinch reflex. Thereafter, the back of each rat was carefully shaved with an electric razor. In the prone position, the animal was placed onto a soft cylindrical bolster, and the flexible template was closely pressed onto the rat's back. One person held the rat and the bolster, the second held the template, and the third pou...

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Section: Discussionmentioning

confidence: 99%

Innovative scald burn model and long-term dressing protector for studies in rats

Pfurtscheller

Petnehazy²,

Goessler³

et al. 2013

Journal of Trauma and Acute Care Surgery

View full text Add to dashboard Cite

show abstract

“…Adicionalmente siguen siendo uno de los modelos de fácil manipulación, disposición, bajo costo de mantenimiento y al ser una especie filogenéticamente menor a las anteriores permiten cumplir con el principio de Refinamiento dentro del principio de las 3R el cual busca garantizar estándares éticos en la investigación con animales (26,27,(31)(32)(33).…”

Section: Introductionunclassified

Evaluación de extractos vegetales para manejo de quemaduras cáusticas: irritación/corrosión dérmica aguda. Bogotá/2018

Céspedes

Ospina

Vargas³

et al. 2018

rev cuarzo

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Londoño Valero10, Laura del Mar Lozada Cruz10 Licencia creative commons ResumenIntroducción: Aunque las quemaduras cáusticas representan un bajo porcentaje de las quemaduras, dadas las complicaciones que asocian, son un problema de salud pública que exige nuevas opciones terapéuticas como son el uso de productos derivados de plantas, entre ellos, Calendula offinalis, Aloe vera y Cannabis sativa, cuyo uso está condicionado, inicialmente a pruebas como la evaluación de su efecto irritativo/corrosivo agudo en piel. Materiales y métodos:Estudio experimental en animales, aleatorizado. El material vegetal se obtuvo del Jardín Medicinal Jorge Piñeros Corpas; los aceites y extractos de las plantas se obtuvieron por métodos clásicos de extracción; la base de excipientes fue provista por el Laboratorio de Farmacología Vegetal (LABFARVE). Se emplearon 8 Ratas wistar macho alojadas bajo condiciones controladas y parámetros de ética y bienestar animal. La prueba de irritación/corrosión dérmica aguda se hizo siguiendo la norma OECD 404, tras previa anestesia de los animales y siguiendo los criterios de dolor y punto final.Resultados: De Calendula officinalis se obtuvieron dos aceites esenciales y tres extractos; el gel de Aloe vera y un extracto de Cannabis sativa. Ninguno de los extractos generó escara, eritema o edema en los animales, siendo negativa la prueba de irritación/corrosión aguda en piel.Discusión: Los resultados obtenidos desde esta investigación básica en medicina traslacional, brindan evidencia que complementa la información reportada en la literatura acerca del uso tradicional y los estudios in vitro e in vivo de estas plantas para el manejo de diversas enfermedades. La ausencia de efecto irritativo o corrosivo en piel es coherente con el conocimiento del tipo de metabolitos secundarios activos que se han identificado en ellas con actividad antiinflamatoria, antioxidante, antimicrobiana y cicatrizante. Los resultados aseguran su inocuidad química al contemplarlas para investigar su potencial uso terapéutico en lesiones dérmicas, tipo quemaduras cáusticas, garantizando su seguridad . AbstractIntroduction: Considering that caustic burns represent a low percentage of burns, given the complications they could associate, they are a public health problem that requires new therapeutic options such as the use of plant-derived products, including Calendula offinalis, Aloe vera and Cannabis sativa, whose use is conditioned, initially to tests such as the evaluation of its acute irritative / corrosive effect on skin.

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Evaluation of biomechanical and histological properties of corrosive chemical burns

Choi

Jang

Lee

2012

Journal of Pharmaceutical Investigation

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A standard experimental ‘chemical burn’

Cited by 5 publications

References 6 publications

Innovative scald burn model and long-term dressing protector for studies in rats

Innovative scald burn model and long-term dressing protector for studies in rats

Evaluación de extractos vegetales para manejo de quemaduras cáusticas: irritación/corrosión dérmica aguda. Bogotá/2018

Evaluation of biomechanical and histological properties of corrosive chemical burns

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