A standard method for DNCB sensitization testing in patients with neoplasms

Bleumink, E.; Jp, Nater; Koops, H. Schraffordt

doi:10.1002/1097-0142(197404)33:4<911::aid-cncr2820330404>3.0.co;2-b

Cited by 57 publications

(26 citation statements)

References 9 publications

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“…Kligman & Balin (50) found that the elderly typically give much weaker reactions to DNCB. Bleumink et al (51) observed that DNCB sensitization was decreased in people over 50 years old.…”

Section: Ii) the Ability To Develop Reactivity Upon Exposure To A Newsupporting

confidence: 73%

Effect of age and sex on the induction and elicitation of allergic contact dermatitis

Kwangsukstith

Maibach

1995

Contact Dermatitis

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This article reviews the effect of age and sex on the induction and elicitation of allergic contact dermatitis (ACD). We found that the incidence of ACD in childhood is lower than in adults; this may be explained by less exposure to allergens, lack of immune response and limited patch test studies in this age group. In the elderly, there is an age-dependent decrease in the incidence of ACD, with decreased sensitization to new allergens and waning responses in previously sensitized subjects. There is extensive controversy about the effect of sex differences in ACD. The induction phases of ACD in both sexes are different due to differences in exposure to the allergens from occupations, habits and cultures. Differences in elicitation phases of ACD in both sexes are not confirmed. Women tend to have a higher incidence of ACD and higher immunologic response to exposure allergens than men. Thus we can only imprecisely define the effect of age and sex on the induction and elicitation of ACD. Further prospective studies should be done to answer these questions.

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“…Kligman & Balin (50) found that the elderly typically give much weaker reactions to DNCB. Bleumink et al (51) observed that DNCB sensitization was decreased in people over 50 years old.…”

Section: Ii) the Ability To Develop Reactivity Upon Exposure To A Newsupporting

confidence: 73%

Effect of age and sex on the induction and elicitation of allergic contact dermatitis

Kwangsukstith

Maibach

1995

Contact Dermatitis

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“…Cell mediated response to dinitrochlorobenzene (DNCB) was assessed as described by Blumink et al (1974). Briefly, on day 14 of age, a primary dose (0.1 mL) of 2% DNCB in acetone was applied on 4 cm 2 area on the skin of each of the five chicks followed by a secondary dose on day 21 of the age (7 days post primary dose).…”

Section: Dinitrochlorobenzene Testmentioning

confidence: 99%

Immunomodulatory Activity of Glycyrrhiza glabra Extract against Mixed Eimeria Infection in Chickens

Hussain¹

2017

IJAB

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This study was conducted to evaluate the immunomodulatory activity of aqueous methanolic extract (AME) of Glycyrrhiza glabra (roots) against mixed Eimeria species infection in broiler chickens. For in vivo trial, 175 day old broiler chicks were divided into five equal groups (A, B, C, D and E). At one week of age, groups A, B and C were given orally three graded doses of G. glabra AME (100, 200 and 300 mg/kg of body weight respectively) for three consecutive days. Group D served as positive (Vitamin E treated) and Group E as negative control (PBS treated). At 14 th day of age, all groups were infected orally with 60,000 sporulated oocysts of mixed Eimeria species. Cell mediated immune response was evaluated by four tests (Phytohemagglutinin-P, Concanavalin-A, Carbon clearance assay and Dinitrochlorobenzene). Humoral immune response was evaluated by microplate hemagglutination test using sheep red blood cells. Results revealed a dose dependent immune response in G. glabra AME treated groups. Cell mediated and humoral immune response of group treated with G. glabra AME @ 300 mg/kg of body weight was almost similar (P˃0.05) to the positive control group (Vitamin E treated). G. glabra AME treated groups showed significantly higher (P<0.05) cell mediated and humoral response as compared to negative control (PBS treated).

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“…To monitor the delayed-type hypersensitivity reaction dinitrochlorobenzene (DNCB) test was used as explained by Blumink et al (1974). In brief, 2% DNCB dose (0.1 mL) prepared in acetone was applied on 4 cm 2 area of skin.…”

Section: Dinitrochlorobenzene (Dncb) Testmentioning

confidence: 99%

Immunological and Therapeutic Evaluation of Wheat (Triticum aestivum) Derived Betaglucans against Coccidiosis in Chicken

Rizwan¹,

Khan²,

Iqbal³

et al. 2016

IJAB

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To cite this paper: Rizwan, H.M., M.K. Khan, Z. Iqbal and F. Deeba, 2016. Immunological and therapeutic evaluation of wheat (Triticum aestivum) derived beta-glucans against coccidiosis in chicken. AbstractThis study was carried out to determine the immunomodulatory and therapeutic effects of wheat derived beta-glucans (purified) against avian coccidiosis. Briefly, beta-glucans from wheat bran were extracted and purified using standard procedures. A total of 250 broiler chicks (day-old) were reared at experimental station, Department of Parasitology, University of Agriculture, Faisalabad for this study. At 7 th day of their age, birds were subdivided into five equal groups (n=50). Groups A, B and C were orally administered with graded doses of purified beta-glucans (50, 100 and 150 mg/kg of body weight) for three consecutive days; whereas, positive control (group D) was administered with vitamin-E at the dose rate of 87mg/kg; while negative control (group E) was served with phosphate buffered saline (PBS solution). At 14 th day post administration of graded doses, 30 chickens from each group were randomly selected and used to monitor cellular and humoral immune responses, while remaining birds (n=20) in each group were challenged with mixed species of genus Eimeria for therapeutic evaluation. Overall, chickens in group A administered with 50 mg/kg b.wt. purified beta-glucans showed significantly higher immune responses in terms of enhanced humoral and cell mediated immunity as compared to those of other beta-glucans administered and E groups. While, the immune responses showed by group A were comparable with group D. After challenge with Eimeria, the groups A and D also showed maximum weight gains with low oocyst counts and maximum percent protection against lesions in both caecum and intestine. On the other hand, a minimum daily weight gain with increased number of oocysts in chickens was observed in control group (PBS) during therapeutic evaluation. These findings suggest that the beta-glucans derived from wheat may have immunomodulatory and therapeutic effects against Eimeria infection in chickens.

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A standard method for DNCB sensitization testing in patients with neoplasms

Cited by 57 publications

References 9 publications

Effect of age and sex on the induction and elicitation of allergic contact dermatitis

Effect of age and sex on the induction and elicitation of allergic contact dermatitis

Immunomodulatory Activity of Glycyrrhiza glabra Extract against Mixed Eimeria Infection in Chickens

Immunological and Therapeutic Evaluation of Wheat (Triticum aestivum) Derived Betaglucans against Coccidiosis in Chicken

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