A standard technique for the isolation ofSalmonellafrom animal feeds

Abstract

SUMMARYTwo trials were carried out by 9 and 47 laboratories to evaluate the reproducibility and sensitivity of a standard technique for the isolation of salmonellas from animal feeding stuffs.The trials involved the examinations of test samples containing known numbers of salmonellas by a buffered peptone water pre-enrichment technique. Statistical analysis of the results of the trials showed that the method was capable of detecting salmonellas in most probable numbers as low as 2·3 per 100 g and that no significant differences in performance were observed between laboratories.The standard technique described is recommended for the examination of animal feed and conforms to international recommendations.

DEVELOPMENT AND EVALUATION OF A RAPID TEST PROCEDURE TO DETECT SALMONELLAE IN FEED INGREDIENTS AND FINISHED FEEDS1

“…Feed manufacturers do not typically have sufficient storage to retain a product for this period of time; therefore, a test providing similar accuracy and sensitivity in a shorter time frame is desirable. Numerous rapid methods have been discussed in the literature (Smith 1977;Harvey and Price 1982;Mulder et al 1983); application of these to animal feeds and feed ingredients needs to be optimized.…”

Combined enrichment procedure for the detection of salmonellae in foods

“…(1976) recovered more salmomellae from minced meat-preenriched in buffered peptone water than those samples placed directly in selective enrichment broths. In another comparative study (Smith, 1977), low numbers of salmonellae were detected using buffered peptone water prior to selective enrichment of animal feeds. Harvey and Price (1977) enhanced the recovery of Salmonella from sewage samples by using buffered peptone water as a preenrichment medium prior to selective enrichment in tetrathionote broth.…”