A standardization of Amphibalanus (Balanus) amphitrite (Crustacea, Cirripedia) larval bioassay for ecotoxicological studies

Piazza, Veronica; Ferioli, Annalisa; Giacco, Elisabetta; Melchiorre, Nunzia; Valenti, Alessandra; Prete, Francesco Del; Biandolino, Francesca; Dentone, Letizia; Frisenda, Paola; Faimali, Marco

doi:10.1016/j.ecoenv.2011.12.014

Cited by 35 publications

(21 citation statements)

References 31 publications

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“…4) were useful to better highlight the slight toxic effect associated to the investigated sediments. Among the model organisms exposed to elutriate, A. amphitrite nauplii resulted to be the most sensitive even considering the sublethal end-point, confirming barnacle nauplii as useful model organisms in toxicity testing (Faimali et al 2006;Piazza et al 2012). SSA test results after 48 h of exposure of A. amphitrite to sediment elutriates allowed to calculate EC 50 values for most of the investigated sediment (1D, 1C, 1E, 1H, 1G, 2B; Table 4).…”

Section: Discussionmentioning

confidence: 97%

“…Crustaceans larvae were obtained in laboratory conditions as reported in Piazza et al (2012) and Garaventa et al (2010), whereas early stages of the rotifer were purchased from MicroBioTests, Inc. (Gent, Belgium), and obtained following the protocol of the manufacturer (Rotoxkit M protocol). For bioassays setup, 10-15 organisms were placed in polystyrene 24 multiwell plates containing 1 ml/well of different FNSW elutriate dilutions (Table 2); A. amphitrite nauplii were incubated at 20±0.5°C and brine shrimps and rotifers at 25± 0.5°C, all in dark conditions.…”

Section: Crustaceans and Rotifers Larvaementioning

confidence: 99%

“…D. tertiolecta is a wellknown and widely used model organism in algal bioassays because of its high sensitivity to several xenobiotics; moreover, this species can be easily maintained in laboratory cultures (US EPA 1974;Walsh 1983;Wong et al 1995). Crustaceans and rotifers larvae are extensively used in ecotoxicological tests because of their ecological relevance and even because they are easy to maintain under laboratory conditions all year round (Sanchez-Fortùn et al 1997;Nunes et al 2006;Garaventa et al 2010;Piazza et al 2012). Sea urchins such as P. lividus are a well-known, sensitive, and reliable model organism.…”

Section: Introductionmentioning

confidence: 99%

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Ecotoxicological effects of sediments from Mar Piccolo, South Italy: toxicity testing with organisms from different trophic levels

Costa

Piazza

Gambardella

et al. 2015

Environ Sci Pollut Res

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The Mar Piccolo of Taranto (Ionian Sea, Southern Italy) is a semi-enclosed and strongly polluted basin. For decades, it has been subjected to different anthropogenic impacts. These stressors caused severe sediments contamination with high concentration of different pollutants (PAHs, PCB, heavy metals). In order to assess the current status of sediments contamination, an ecotoxicological investigation combined with chemical analysis (heavy metals, PAH, and PCB) has been performed. In order to derive ecologically relevant conclusions, a multiorganisms and multiend-points approach has been applied, exposing organisms from different trophic levels to elutriate and whole sediment. The battery of bioassays consists of a microalgal growth inhibition test (Dunaliella tertiolecta), acute and sublethal assays (end-points: mortality, immobilization and swimming speed alteration) on crustaceans larvae and juveniles, and rotifers (Amphibalanus amphitrite, Artemia salina, Corophium insidiosum and Brachionus plicatilis), and embryotoxicity test on echinoderms (Paracentrotus lividus). Considering the high levels of sediment contamination highlighted from chemical analysis, an unexpected very low toxic effect was observed, even considering the sublethal end-point (larval swimming speed alteration). The results of this study suggest a very complex contaminants dynamic in the Mar Piccolo sediments that, despite a strong level of contamination, seems to not affect in a proportional manner the biological compartment.

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Section: Discussionmentioning

confidence: 97%

Section: Crustaceans and Rotifers Larvaementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ecotoxicological effects of sediments from Mar Piccolo, South Italy: toxicity testing with organisms from different trophic levels

Costa

Piazza

Gambardella

et al. 2015

Environ Sci Pollut Res

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“…amphitrite is the ideal species to be proposed as an excellent model organism for ecotoxicity tests (Faimali et al, 2005;Garaventa et al, 2010;Greco et al, 2006;Piazza et al, 2009Piazza et al, , 2012, for it has a wide geographic distribution, ecological relevance, it is easy to rear (its life cycle can be easily reproduced in laboratory conditions), it has a relatively fast larval development, and a high sensitivity to a wide range of toxicants, such as antifouling biocides, heavy metals, neurotoxic pesticides and surfactants (Greco et al, 2006;Faimali et al, 2005Faimali et al, , 2007.…”

Section: Model Organismmentioning

confidence: 99%

“…An ecotoxicological bioassay with larvae of the crustacean A. amphitrite was recently developed and standardized in our laboratories (Piazza et al, 2012). The Italian regulatory authority UNICHIM has published it as an official protocol (NU 2245/2012), recommending specific temperature and salinity values (20°C and 37‰, respectively).…”

Section: Introductionmentioning

confidence: 99%

Temperature and salinity effects on cadmium toxicity on lethal and sublethal responses of Amphibalanus amphitrite nauplii

Piazza

Gambardella

Canepa

et al. 2016

Ecotoxicology and Environmental Safety

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Cold storage effects on lethal and sublethal responses of Amphibalanus amphitrite Nauplii

et al. 2022

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Bioassays are extensively used in ecotoxicology and there is a constant need for even more sensitive, reliable and easy to rear and obtain model organisms. Larvae of the crustacean Amphibalanus amphitrite are a good ecotoxicological model, for their high sensitivity to a wide range of toxicants and emerging contaminants. A standardized protocol for this toxicity bioassay has been recently proposed. Nevertheless, a limit of this model organism is the lack of resting stages and the need to use larvae immediately after their release from adults, thus increasing laboratory efforts related to the maintenance of adults. The aim of this work is to verify if short-term cold storage of A. amphitrite larvae prior to use in ecotoxicological tests may affect the ecotoxicological responses of these organisms. Three end-points (mortality, immobilization and swimming speed alteration) were measured on nauplii after storing them at 4 ± 1 °C for different times (24, 72 and 120 h) before bioassay set-up. Bioassays were set up using: (i) clean filtered natural sea water (0.22 µm FNSW), (ii) a reference toxicant (Cadmium Nitrate) and (iii) an environmental matrix (sediment elutriate). Results show that mortality, differently from the other two endpoints, was not affected by cold-storage. Even after 5 days of larvae storage at 4 ± 1 °C before bioassay set up, mortality data were comparable to those obtained for non-cold-stored organisms. Moreover, larval sensitivity to the reference toxicant and sediment elutriate did not change. Regarding the other two end points, low cadmium concentrations significantly changed immobility and swimming activity in cold-stored nauplii compared to larvae used immediately after larval release. In conclusion, short-term cold storage of A. amphitrite nauplii before bioassay set up is an appropriate procedure in ecotoxicological testing if mortality is the endpoint to be considered for final evaluation.

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A standardization of Amphibalanus (Balanus) amphitrite (Crustacea, Cirripedia) larval bioassay for ecotoxicological studies

Cited by 35 publications

References 31 publications

Ecotoxicological effects of sediments from Mar Piccolo, South Italy: toxicity testing with organisms from different trophic levels

Ecotoxicological effects of sediments from Mar Piccolo, South Italy: toxicity testing with organisms from different trophic levels

Temperature and salinity effects on cadmium toxicity on lethal and sublethal responses of Amphibalanus amphitrite nauplii

Cold storage effects on lethal and sublethal responses of Amphibalanus amphitrite Nauplii

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