A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study

Shirato, Ken; Takanari, Jun; Koda, Tomoko; Sakurai, Takeshi; Ogasawara, Jun; Ohno, Hideki; Kizaki, Takako

doi:10.1186/s12199-018-0730-3

Cited by 7 publications

(14 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No reuse allowed without permission. [21,[24][25][26][27]. To assess its anti-inflammatory effects, the cells were co-treated with the indicated concentrations of ETAS®50 or dextrin and 100 ng/mL of SARS-CoV-2 spike recombinant protein S1 subunit (Arigo Biolaboratories, Hsinchu City, Taiwan) for 1 to 24 h.…”

Section: Agents and Treatment Different Concentrations Of Etas®50 Or Dextrin (Vehicle Control)mentioning

confidence: 99%

“…(0.25, 0.5, 1, or 2 mg/mL) were prepared by directly dissolving each agent in the complete medium, and then the supplemented medium was filter sterilized using a 0.22 μm membrane [21,[24][25][26][27]. To assess its anti-inflammatory effects, the cells were co-treated with the indicated concentrations of ETAS®50 or dextrin and 100 ng/mL of SARS-CoV-2 spike recombinant protein S1 subunit (Arigo Biolaboratories, Hsinchu City, Taiwan) for 1 to 24 h. respectively.…”

Section: Agents and Treatment Different Concentrations Of Etas®50 Or Dextrin (Vehicle Control)mentioning

confidence: 99%

“…Interestingly, a recent report showed that ETAS®50 supplementation restores cognitive functions and prevents neuronal apoptosis in the hippocampus of transgenic mice overexpressing amyloid precursor protein, which mimics Alzheimer's disease [20]. The in vivo protective actions of ETAS®50 are suggested to be mediated by its ability to induce the expression of heat-shock protein 70 (HSP70) [17,18,20,21], and asfral [22] and asparaprolines [23] have been identified as compounds that can induce HSP70 expression at the cellular and individual levels.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

Shirato

Takanari

Kizaki

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Excessive host inflammation following infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is associated with severity and mortality in coronavirus disease 2019 (COVID-19). We recently reported that the SARS-CoV-2 spike protein S1 subunit (S1) induces pro-inflammatory responses by activating toll-like receptor 4 (TLR4) signaling in macrophages. ETAS®50, a standardized extract of Asparagus officinalis stem, is a unique functional food that elicits anti-photoaging effects by suppressing pro-inflammatory signaling in hydrogen peroxide- and ultraviolet B-exposed skin fibroblasts. To elucidate its potential in preventing excessive inflammation in COVID-19, we examined the effects of ETAS®50 on pro-inflammatory responses in S1-stimulated murine peritoneal exudate macrophages. Co-treatment of the cells with ETAS®50 significantly attenuated S1-induced secretion of interleukin (IL)-6 in a concentration-dependent manner without reducing cell viability. ETAS®50 also markedly suppressed the S1-induced transcription of IL-6 and IL-1β. However, among the TLR4 signaling proteins, ETAS®50 did not affect the degradation of inhibitor κBα, nuclear translocation of nuclear factor-κB p65 subunit, and phosphorylation of c-Jun N-terminal kinase p54 subunit after S1 exposure. In contrast, ETAS®50 significantly suppressed S1-induced phosphorylation of p44/42 mitogen-activated protein kinase (MAPK) and Akt. Attenuation of S1-induced transcription of IL-6 and IL-1β by the MAPK kinase inhibitor U0126 was greater than that by the Akt inhibitor perifosine, and the effects were potentiated by simultaneous treatment with both inhibitors. These results suggest that ETAS®50 attenuates S1-induced IL-6 and IL-1β production by suppressing p44/42 MAPK and Akt signaling in macrophages. Therefore, ETAS®50 may be beneficial in regulating excessive inflammation in patients with COVID-19.

show abstract

Section: Agents and Treatment Different Concentrations Of Etas®50 Or Dextrin (Vehicle Control)mentioning

confidence: 99%

Section: Agents and Treatment Different Concentrations Of Etas®50 Or Dextrin (Vehicle Control)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

Shirato

Takanari

Kizaki

2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Interestingly, a recent report showed that EAS supplementation restores cognitive functions and prevents neuronal apoptosis in the hippocampus of transgenic mice overexpressing amyloid precursor protein, which mimics Alzheimer’s disease [ 20 ]. The in vivo protective actions of EAS are suggested to be mediated by its ability to induce the expression of heat-shock protein 70 (HSP70) [ 17 , 18 , 20 , 21 ], and asfral [ 22 ] and asparaprolines [ 23 ] have been identified as compounds that can induce HSP70 expression at the cellular and individual levels.…”

Section: Introductionmentioning

confidence: 99%

Standardized Extract of Asparagus officinalis Stem Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

2021

Self Cite

View full text Add to dashboard Cite

Excessive host inflammation following infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is associated with severity and mortality in coronavirus disease 2019 (COVID-19). We recently reported that the SARS-CoV-2 spike protein S1 subunit (S1) induces pro-inflammatory responses by activating toll-like receptor 4 (TLR4) signaling in macrophages. A standardized extract of Asparagus officinalis stem (EAS) is a unique functional food that elicits anti-photoaging effects by suppressing pro-inflammatory signaling in hydrogen peroxide and ultraviolet B-exposed skin fibroblasts. To elucidate its potential in preventing excessive inflammation in COVID-19, we examined the effects of EAS on pro-inflammatory responses in S1-stimulated macrophages. Murine peritoneal exudate macrophages were co-treated with EAS and S1. Concentrations and mRNA levels of pro-inflammatory cytokines were assessed using enzyme-linked immunosorbent assay and reverse transcription and real-time polymerase chain reaction, respectively. Expression and phosphorylation levels of signaling proteins were analyzed using western blotting and fluorescence immunomicroscopy. EAS significantly attenuated S1-induced secretion of interleukin (IL)-6 in a concentration-dependent manner without reducing cell viability. EAS also markedly suppressed the S1-induced transcription of IL-6 and IL-1β. However, among the TLR4 signaling proteins, EAS did not affect the degradation of inhibitor κBα, nuclear translocation of nuclear factor-κB p65 subunit, and phosphorylation of c-Jun N-terminal kinase p54 subunit after S1 exposure. In contrast, EAS significantly suppressed S1-induced phosphorylation of p44/42 mitogen-activated protein kinase (MAPK) and Akt. Attenuation of S1-induced transcription of IL-6 and IL-1β by the MAPK kinase inhibitor U0126 was greater than that by the Akt inhibitor perifosine, and the effects were potentiated by simultaneous treatment with both inhibitors. These results suggest that EAS attenuates S1-induced IL-6 and IL-1β production by suppressing p44/42 MAPK and Akt signaling in macrophages. Therefore, EAS may be beneficial in regulating excessive inflammation in patients with COVID-19.

show abstract

“…Melanin is synthesized from melanocytes in various sense organs including the skin in animals [2]. Previous studies have shown the protective effects of melanin in the skin against ultraviolet light irradiation [3, 4]. Previous studies have also shown adsorption of toxic elements such as mercury, lead [5], cadmium [6], and barium [7] by melanin.…”

Section: Introductionmentioning

confidence: 99%

Adsorption of molybdenum by melanin

Chen

Hashimoto

Omata

et al. 2019

Environ Health Prev Med

View full text Add to dashboard Cite

Background Melanin is detectable in various sense organs including the skin in animals. It has been reported that melanin adsorbs toxic elements such as mercury, cadmium, and lead. In this study, we investigated the adsorption of molybdenum, which is widely recognized as a toxic element, by melanin. Methods Molybdenum level of the mouse skin was measured by inductively coupled plasma mass spectrometry. The pigmentation level of murine skin was digitalized as the L * value by using a reflectance spectrophotometer. An in vitro adsorption assay was performed to confirm the interaction between molybdenum and melanin. Results Our analysis of hairless mice with different levels of skin pigmentation showed that the level of molybdenum increased with an increase in the level of skin pigmentation ( L * value). Moreover, our analysis by Spearman’s correlation coefficient test showed a strong correlation ( r = − 0.9441, p < 0.0001) between L * value and molybdenum level. Our cell-free experiment using the Langmuir isotherm provided evidence for the adsorption of molybdenum by melanin. The maximum adsorption capacity of 1 mg of synthetic melanin for molybdenum was 131 μg in theory. Conclusion Our in vivo and in vitro results showed a new aspect of melanin as an adsorbent of molybdenum. Electronic supplementary material The online version of this article (10.1186/s12199-019-0791-y) contains supplementary material, which is available to authorized users.

show abstract

A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study

Cited by 7 publications

References 12 publications

ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

Standardized Extract of Asparagus officinalis Stem Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

Adsorption of molybdenum by melanin

Contact Info

Product

Resources

About