A standardized method for the sampling of rhizosphere and rhizoplan soil bacteria associated to a herbaceous root system

Barillot, Cindy; Sarde, Claude-Olivier; Bert, Valérie; Tarnaud, Eric; Cochet, N.

doi:10.1007/s13213-012-0491-y

Cited by 179 publications

(91 citation statements)

References 34 publications

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“…; the leaves and roots sampled were processed for the chemical investigations in accordance with the procedures proposed by Visoottiviseth et al . and Barillot et al ., respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Soil samples were collected from the HM contaminated plots (P2, P3 and P4) following the sampling procedure reported in detail in Ancona et al 48 Plant materials were also sampled from each plot studied: roots were collected from the rhizosphere (soil surrounding the root system ∼50 cm 2 around the trunk) and stems and leaves from the outer canopy located in the upper third of each target plant, corresponding to the area in which mineral elements are mainly stored because of the higher transpiration rates. 49 The collection of the plant materials was performed in accordance with Ancona et al; 48 the leaves and roots sampled were processed for the chemical investigations in accordance with the procedures proposed by Visoottiviseth et al 50 and Barillot et al, 51 respectively.…”

Section: Sampling Of Soil and Plant Materialsmentioning

confidence: 99%

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Heavy metal phytoremediation of a poplar clone in a contaminated soil in southern Italy

Ancona

Caracciolo

Campanale

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The Monviso clone previously tested successfully to remediate a lindane‐contaminated soil in central Italy was then applied in a historically heavy metal contaminated area in southern Italy. RESULTS The results obtained at 900 days from the poplar planting showed a strong decrease below Italian national legal limits (D.Lgs 152/06) for most heavy metals (HMs). The highest reduction of HM concentrations was observed in soil samples collected near the trunk (25 cm distant from poplar) and in the rhizosphere. The values of the HM bioaccumulation factor (BAF, considering roots and leaves) and of the translocation factor (TF) suggest that poplar trees were able to phytostabilize HMs. The soil organic carbon content increased compared to the previous sampling in all plots planted. The highest values were observed for the rhizosphere soils of each target tree, while the phosphorous available strongly decreased. Microbial results showed an overall increase in cell viability, dehydrogenase activity and microbial abundance of some bacterial groups. CONCLUSION Overall results showed that the poplar‐based phytoremediation strategy was able to promote the phytostabilization of the HMs and to improve the soil quality in terms of both organic carbon content and microbial community structure and activity. © 2019 Society of Chemical Industry

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“…; the leaves and roots sampled were processed for the chemical investigations in accordance with the procedures proposed by Visoottiviseth et al . and Barillot et al ., respectively.…”

Section: Methodsmentioning

confidence: 99%

Section: Sampling Of Soil and Plant Materialsmentioning

confidence: 99%

Heavy metal phytoremediation of a poplar clone in a contaminated soil in southern Italy

Ancona

Caracciolo

Campanale

et al. 2019

J of Chemical Tech & Biotech

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“…The resultant soil suspension was used to determinate the root-free soil microbial population. Three individual samples for each single plot were collected at the end of the experiment and analysed separately (Barillot et al 2012). Soil samples were examined to determine: the total number of bacteria, number of gram-negative bacteria, Pseudomonas, nitrate decomposing bacteria, sulphur amino-acid decomposing bacteria and the number of fungi.…”

Section: Microbial Investigationmentioning

confidence: 99%

Ecological strategy for soil contaminated with mercury

et al. 2016

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Aims The paper presents results from plot experiments aimed at the development of an ecological strategy for soil contaminated with mercury. Meadow grass (Poa pratensis) was tested on mercury contaminated soil in a former chlor-alkali plant (CAP) in southern Poland for its phytoremediation potential. Methods The stabilisation potential of the plants was investigated on plots without additives and after the addition of granular sulphur. Biomass production, uptake and distribution of mercury by plants, as well as leachates and rhizosphere microorganisms were investigated, along with the growth and vitality of plants during one growing season. Results The analysed plants grew easily on mercury contaminated soil, accumulating lower amounts of mercury, especially in the roots, from soil with additive of granular sulphur (0.5 % w/w) and sustained a rich microbial population in the rhizosphere. After amendment application the reduction of Hg evaporation was observed.Conclusions The obtained results demonstrate the potential of using Poa pratensis and sulphur for remediation of mercury contaminated soil and reduction of the Hg evaporation from soil. In the presented study, methods of Hg reduction on Bhot spots^were proposed, with a special focus on environmental protection. This approach provides a simple remediation tool for large areas heavily contaminated with mercury.

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“…The Cd tolerant and hyperaccumulating species A. halleri was chosen as model plant for this study because this species is naturally present in metalcontaminated soils of this studied area. Moreover, we have some knowledge on Zn and Cd tolerance and accumulation (Sarret et al, 2009 andIsaure et al, 2015, respectively), and on rhizospheric processes (Barillot et al, 2013) for A. halleri from the same metallicolous origin.…”

mentioning

confidence: 99%

Fate of cadmium in the rhizosphere of Arabidopsis halleri grown in a contaminated dredged sediment

Huguet

Isaure

Bert

et al. 2015

Science of The Total Environment

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In regions impacted by mining and smelting activities, dredged sediments are often contaminated with metals. Phytotechnologies could be used for their management, but more knowledge on the speciation of metals in the sediment and on their fate after colonization by plant roots is needed. This work was focused on a dredged sediment from the Scarpe river (North of France), contaminated with Zn and Cd. Zn, Cd hyperaccumulating plants Arabidopsis halleri from metallicolous and non-metallicolous origin were grown on the sediment for five months in a pot experiment. The nature and extent of the modifications in Cd speciation with or without plant were determined by electron microscopy, micro X-ray fluorescence and bulk and micro X-ray absorption spectroscopy. In addition, changes in Cd exchangeable and bioavailable pools were evaluated, and Cd content in leachates was measured. Finally, Cd plant uptake and plant growth parameters were monitored. In the original sediment, Cd was present as a mixed Zn, Cd, Fe sulfide. After five months, although pots still contained reduced sulfur, Cd-bearing sulfides were totally oxidized in vegetated pots, whereas a minor fraction (8%) was still present in non-vegetated ones. Secondary species included Cd bound to O-containing groups of organic matter and Cd phosphates. Cd exchangeability and bioavailability were relatively low and did not increase during changes in Cd speciation, suggesting that Cd released by sulfide oxidation was readily taken up with strong interactions with organic matter and phosphate ligands. Thus, the composition of the sediment, the oxic conditions and the rhizospheric activity (regardless of the plant origin) created favorable conditions for Cd stabilization. However, it should be kept in mind that returning to anoxic conditions may change Cd speciation, so the species formed cannot be considered as stable on the long term.

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A standardized method for the sampling of rhizosphere and rhizoplan soil bacteria associated to a herbaceous root system

Cited by 179 publications

References 34 publications

Heavy metal phytoremediation of a poplar clone in a contaminated soil in southern Italy

Heavy metal phytoremediation of a poplar clone in a contaminated soil in southern Italy

Ecological strategy for soil contaminated with mercury

Fate of cadmium in the rhizosphere of Arabidopsis halleri grown in a contaminated dredged sediment

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