A State of the Art Review of Isochoric Cryopreservation and Cryoprotectants

Beșchea, George-Andrei; Câmpean, Ştefan-Ioan; Tăbăcaru, Maria-Bianca; Vuţoiu, Beatrice-Georgiana; Şerban, Alexandru; Năstase, Gabriel

doi:10.54680/fr22410110112

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…However, although the microbiological results are promising, these treatments damage cells, also at the DNA level [4], degrading the food. Moreover, advanced techniques of freezing have been implemented, such as the isochoric method [6]. This new method of freezing can preserve food without causing damage due to ice-crystal formation inside it, although it can generate hydrostatic pressures inside the processing chamber, deteriorating the food quality [7].…”

Section: Introductionmentioning

confidence: 99%

Combining Blue Light and Yellow Curcumin to Obtain a “Green” Tool for Berry Preservation against Bacterial Contamination: A Preliminary Investigation

Stura

Munir

Cavallo

et al. 2023

Foods

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Background: According to recent studies, tens of millions of tons of fruit are wasted each year in Europe in primary production and home/service consumption. Among fruits, berries are most critical because they have a shorter shelf life and a softer, more delicate, and often edible skin. Curcumin is a natural polyphenolic compound extracted from the spice turmeric (Curcuma longa L.) which exhibits antioxidant, photophysical, and antimicrobial properties that can be further enhanced by photodynamic inactivation of pathogens when irradiated with blue or ultraviolet light. Materials and methods: Multiple experiments were performed in which berry samples were sprayed with a complex of β-cyclodextrin containing 0.5 or 1 mg/mL of curcumin. Photodynamic inactivation was induced by irradiation with blue LED light. Antimicrobial effectiveness was assessed with microbiological assays. The expected effects of oxidation, curcumin solution deterioration, and alteration of the volatile compounds were investigated as well. Results: The treatment with photoactivated curcumin solutions reduced the bacterial load (3.1 vs. 2.5 colony forming units/mL (UFC/ml) in the control and treated groups; p-value = 0.01), without altering the fruit organoleptic and antioxidant properties. Conclusions: The explored method is a promising approach to extend berries’ shelf life in an easy and green way. However, further investigations of the preservation and general properties of treated berries are still needed.

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Section: Introductionmentioning

confidence: 99%

Combining Blue Light and Yellow Curcumin to Obtain a “Green” Tool for Berry Preservation against Bacterial Contamination: A Preliminary Investigation

Stura

Munir

Cavallo

et al. 2023

Foods

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Liquid–solid equilibria and supercooling of Custodiol® in isochoric thermodynamic systems at subfreezing temperatures

Câmpean,

Beșchea,

Tăbăcaru

et al. 2023

Physics of Fluids

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There is growing interest in using isochoric freezing and isochoric supercooling for the preservation of biological matter at subfreezing temperatures. Custodiol® is a commonly used intracellular composition type, subnormothermic preservation solution. It is anticipated that Custodiol® will also be used for isochoric freezing and isochoric supercooling preservation of biological matter. The thermodynamic properties of Custodiol® at subfreezing temperatures as well as the metastable behavior of the solution at subfreezing temperatures were not studied in the past. This study was designed to generate the thermodynamic data needed for the use of Custodiol® for the preservation of biological matter in isochoric systems at subfreezing temperatures. The experiments were performed in a specially designed isochoric chamber that can measure simultaneously the temperature and pressure in the isochoric chamber, and thereby correlate pressure and temperature at thermodynamic equilibrium in isochoric systems as well as the nucleation temperature in isochoric supercooling. The primary focus of this study is on determining the temperature at which nucleation is initiated and to identify the temperature threshold for nucleation due to its specific relevance to various applications in medicine.

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Snow Resource Reutilization: Design of Snow Collection and Compression Equipment Based on Functional Analysis Method

Zhang,

Guo,

Zhu

et al. 2024

Sustainability

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High-latitude regions of the Earth are rich in natural snow resources; however, owing to their negative impact on daily life, they have not been effectively utilized for a long time and are instead viewed as obstacles that require substantial resources for clearance. This waste of resources contradicts the principles of sustainable development. With the rapid development of the ice and snow industry, the social and economic value of snow resources is gradually becoming apparent. Therefore, to promote sustainable social and economic development, this study explores new methods for processing snow resources to achieve their recycling and high-value transformation. This study employs the functional analysis method to analyze and solve the functions of the design system, utilizing the Analytic Hierarchy Process (AHP) and fuzzy comprehensive evaluation for the objective assessment and selection of schemes. The obtained relative optimal principles are utilized to guide the parameter setting and innovative design of the scheme in terms of functional structure. After the final scheme is output, its feasibility is verified through finite element simulation. Ultimately, to address the issue of snow resource clearing and recycling, this study designs a product scheme capable of collecting and compressing snow on road surfaces, proposing the use of snow in the form of standardized compressed snow blocks for large-scale snow sculpture construction and other fields. This method significantly reduces the cost of snow sculpture production, enhances efficiency, realizes the comprehensive utilization and high-value transformation of snow resources, and provides a reference for the sustainable development of the low-altitude ice and snow tourism industry.

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A State of the Art Review of Isochoric Cryopreservation and Cryoprotectants

Cited by 3 publications

References 22 publications

Combining Blue Light and Yellow Curcumin to Obtain a “Green” Tool for Berry Preservation against Bacterial Contamination: A Preliminary Investigation

Combining Blue Light and Yellow Curcumin to Obtain a “Green” Tool for Berry Preservation against Bacterial Contamination: A Preliminary Investigation

Liquid–solid equilibria and supercooling of Custodiol® in isochoric thermodynamic systems at subfreezing temperatures

Snow Resource Reutilization: Design of Snow Collection and Compression Equipment Based on Functional Analysis Method

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