A Statistical Design Approach for Xylanase Production by Aspergillus Niger Using Soybean Hulls : Optimization and Determining the Synergistic Effects of Medium Components on the Enzyme Production

Abstract

Xylanases are hydrolytic enzymes produced by different microorganisms which convert xylooligomers to its constituent xylose units. The growing interests in xylanase production could be linked to its diverse industrial applications. The use of agricultural residues contributed in overcoming one of the major challenges of enzyme production, which is the production cost. This study involves the use of Face centered central composite design (FCCCD) under Response surface methodology (RSM) to optimize the medium components for enhanced xylanase production using soybean hulls as renewable substrate by solid state fermentation. The optimum components that led to maximum xylanase activity of 139.73 U/g were 0.58% w/w of urea, 0.03% w/w of K 2 HPO 4 and 0.04% w/w of Na 2 CO 3. Coefficient of determination (R 2) used to check the fitness of the model showed the correlation between the experimental and predicted responses with a value of 0.8981. Thus, this study showed the potential use of soybean hulls for xylanase production Keywords optimization, renewable substrate, soybean hulls, xylanase.

Statistical optimization of xylanase production, using different agricultural wastes by Aspergillus oryzae MN894021, as a biological control of faba bean root diseases

“…Obtained results were greater than those of Park et al (2002) who denoted that the enhanced xylanase activity of 0.5 U/ml, using rice straw by A. niger culture, was achieved by fractional factorial design of optimization medium conditions and process parameters. As well, the present results were the most close to those obtained by Salihu et al (2015) who reported that the maximum xylanase activity varied from 1.00 to 1.5 U/ml, using soybean hulls by A. fumigatus NITDGPKA3 and A. niger, respectively, using statistical methods.…”

Enhanced endoxylanase production by Myceliophthora thermophila with applicability in saccharification of agricultural substrates

“…The influence of di-potassium salt, K 2 HPO 4 , and its concentrations on endoxylanase production were determined and it was found that increased (2191.28 U/g DR) endoxylanase production was obtained by addition of 0.5% K 2 HPO 4 in moistening medium (Table 1). Salihu et al (2015) also reported that K 2 HPO 4 was one of most important components for obtaining maximum xylanase production from Aspergillus niger AS-1. Jeya et al (2005) also found that K 2 HPO 4 significantly increased the production of endoxylanase from Aspergillus versicolor MKU3.…”

Optimization of novel halophilic lipase production by Fusarium solani strain NFCCL 4084 using palm oil mill effluent

“…The optimization production of lipase by utilizing POME-based medium was achieved by using FCCCD under response surface methodology (RSM) [38], [39]. The design was used to determine the interaction as well as to optimize three independent quantitative variables viz ; sodium nitrate (X1), Tween-80 (X2) and di-potassium hydrogen phosphate (X3) which were identified to exhibit most significant positive effect following PB and OFAT screening.…”