A stem-loop like aptasensor for sensitive detection of aflatoxin based on graphene oxide/AuNPs nanocomposite platform

Dadmehr, Mehdi; Shahi, Sahar Cheraghi; Malekkiani, Mitra; Korouzhdehi, Behnaz; Tavassoli, Abolfazl

doi:10.1016/j.foodchem.2022.134212

Cited by 57 publications

(7 citation statements)

References 27 publications

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“…After the immobilization of aptamer (curve c, Apt/AuNPs/PCs/GCE), an obvious decrease in peak current is found. It could be explained by the negatively charged phosphate groups in the aptamer backbone, which obstructs the negatively charged redox probe [Fe(CN) 6 ] 3−/4− from reaching electrode surface [ 25 ]. Subsequently, when MCH is added to block nonspecific sites, the peak current shows a further decrease because of the non-conductive property of MCH (curve d, MCH/Apt/AuNPs/PCs/GCE).…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Aptasensor Based on Au Nanoparticles Decorated Porous Carbon Derived from Metal-Organic Frameworks for Ultrasensitive Detection of Chloramphenicol

et al. 2022

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A facile and sensitive electrochemical aptamer sensor (aptasensor) based on Au nanoparticles-decorated porous carbon (AuNPs/PC) composite was developed for the efficient determination of the antibiotic drug chloramphenicol (CAP). AuNPs modified metal-organic framework (AuNPs/ZIF-8) is applied as a precursor to synthesize the porous carbon with homogeneous AuNPs distribution through a direct carbonization step under nitrogen atmosphere. The as-synthesized AuNPs/PC exhibits high surface area and improved conductivity. Moreover, the loading AuNPs could enhance the attachment of the aptamers on the surface of electrode through the Au–S bond. When added to CAP, poorly conductive aptamer-CAP complexes are formed on the sensor surface, which increases the hindrance to electron transfer resulting in a decrease in electrochemical signal. Based on this mechanism, the developed CAP aptasensor represents a wide linear detection range of 0.1 pM to 100 nM with a low detection limit of 0.03 pM (S/N = 3). In addition, the proposed aptasensor was employed for the analysis of CAP in honey samples and provided satisfactory recovery.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Aptasensor Based on Au Nanoparticles Decorated Porous Carbon Derived from Metal-Organic Frameworks for Ultrasensitive Detection of Chloramphenicol

et al. 2022

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“…After continuous research, in 2015, Zhan [40] and his research team adjusted the aspect ratio of AuNPs so that the absorption peaks of the long and short axes overlapped with the excitation source and emission position of the upconversion nanoparticles, and controlled the particle size of the upconversion nanoparticles to 4 nm to ensure that all the doped ions were affected by the electromagnetic field of the SPR to enhance the intensity of the upconversion emission. Dadmehr [41] et al conjugated the aptamers to deposit on the surface of graphene oxide decorated with AuNPs, and the following formation of a hetero-duplex stem-loop structure led to fluorescence quenching. Moreover, Hu et al [42] also reported a sensitive fluorescent probe with off-on FRET response for harmful thiourea that can be detected by fluorescent carbon nanodots combined with AuNPs via electrostatic interaction.…”

Section: Förster Resonance Energy Transfer (Fret)mentioning

confidence: 99%

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Chan

Chang

et al. 2023

Molecules

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Molecular biology applications based on gold nanotechnology have revolutionary impacts, especially in diagnosing and treating molecular and cellular levels. The combination of plasmonic resonance, biochemistry, and optoelectronic engineering has increased the detection of molecules and the possibility of atoms. These advantages have brought medical research to the cellular level for application potential. Many research groups are working towards this. The superior analytical properties of gold nanoparticles can not only be used as an effective drug screening instrument for gene sequencing in new drug development but also as an essential tool for detecting physiological functions, such as blood glucose, antigen-antibody analysis, etc. The review introduces the principles of biomedical sensing systems, the principles of nanomaterial analysis applied to biomedicine at home and abroad, and the chemical surface modification of various gold nanoparticles.

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“…Based on this principle, rapid detection methods for AFB 2 , OTA, and AFB 1 were developed [ 185 , 186 ]. Mehdi Dadmehr and co-workers utilized carboxyfluorescein (FAM) tags to label specific aptamers with internal complementary sequences [ 187 ]. This aptamer was further modified onto the surface of a GO/AuNPs nanocomposite to form a heterogenous double-stranded stem–loop structure, resulting in fluorescence quenching.…”

Section: Nanoscale Materials For Rapid Detection and Screeningmentioning

confidence: 99%

Nanoscale Materials Applying for the Detection of Mycotoxins in Foods

Hu,

Li,

Yang

et al. 2023

Foods

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Trace amounts of mycotoxins in food matrices have caused a very serious problem of food safety and have attracted widespread attention. Developing accurate, sensitive, rapid mycotoxin detection and control strategies adapted to the complex matrices of food is crucial for in safeguarding public health. With the continuous development of nanotechnology and materials science, various nanoscale materials have been developed for the purification of complex food matrices or for providing response signals to achieve the accurate and rapid detection of various mycotoxins in food products. This article reviews and summarizes recent research (from 2018 to 2023) on new strategies and methods for the accurate or rapid detection of mold toxins in food samples using nanoscale materials. It places particular emphasis on outlining the characteristics of various nanoscale or nanostructural materials and their roles in the process of detecting mycotoxins. The aim of this paper is to promote the in-depth research and application of various nanoscale or structured materials and to provide guidance and reference for the development of strategies for the detection and control of mycotoxin contamination in complex matrices of food.

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A stem-loop like aptasensor for sensitive detection of aflatoxin based on graphene oxide/AuNPs nanocomposite platform

Cited by 57 publications

References 27 publications

Electrochemical Aptasensor Based on Au Nanoparticles Decorated Porous Carbon Derived from Metal-Organic Frameworks for Ultrasensitive Detection of Chloramphenicol

Electrochemical Aptasensor Based on Au Nanoparticles Decorated Porous Carbon Derived from Metal-Organic Frameworks for Ultrasensitive Detection of Chloramphenicol

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Nanoscale Materials Applying for the Detection of Mycotoxins in Foods

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