A Stepwise Targeting and Antibacterial Strategy by Leukocyte Membrane-Based Conjugated Oligomer Nanoparticles

Abstract

Bacterial infection poses an enormous threat to human life and health. The inability of drugs to be effectively delivered to the site of infection and the development of bacterial resistance make the treatment process more difficult. Herein, a stepwise targeted biomimetic nanoparticle (NPs@M-P) with inflammatory tendency and Gram-negative bacterial targeting was designed, which can achieve efficient antibacterial activity under near-infrared triggering. Leukocyte membranes and targeted molecules (PMB) are used to deliver NPs to the surface of Gram-negative bacteria. The heat and ROS released by NPs@M-P can efficiently kill Gram-negative bacteria under lowpower near-infrared light. Thus, this multimodal combination therapy strategy has broad promise in fighting bacterial infection and avoiding drug resistance.

Microenvironment with NIR-Controlled ROS and Mechanical Tensions for Manipulating Cell Activities in Wound Healing

“…32,33 To endow it with advances of the NIR responsive property, we fabricate a hydrogel composite based on PIC and ETTC conjugated polymer nanoparticles. As illustrated in Scheme 1a, ETTC NPs were prepared by nanoprecipitation together with amphiphilic polymer (DSPE-PEG-2000) 34 and exhibit a broad NIR absorption spectrum with a prominent shoulder at 808 nm (Figure S1). Whereafter, NPs were encapsulated within cell membrane extracted from extracted from skin fibroblast L929 cells to form the camouflaged CM@ NPs using ultrasound.…”

“…Because of these highly biomimetic characteristics, previous studies have demonstrated that PIC hydrogels can respond to cell tractions to form bidirectional cell–matrix interactions and facilitate connective tissue regeneration in an abdominal wound model and stay adherent to the wound without additional support. , To endow it with advances of the NIR responsive property, we fabricate a hydrogel composite based on PIC and ETTC conjugated polymer nanoparticles. As illustrated in Scheme a, ETTC NPs were prepared by nanoprecipitation together with amphiphilic polymer (DSPE-PEG-2000) and exhibit a broad NIR absorption spectrum with a prominent shoulder at 808 nm (Figure S1). Whereafter, NPs were encapsulated within cell membrane extracted from extracted from skin fibroblast L929 cells to form the camouflaged CM@NPs using ultrasound .…”