A stereologic study of postmature placentas fixed by dual perfusion

Larsen, Lise Grupe; Clausen, Helle V.; Andersen, Birthe; Græm, Niels

doi:10.1016/0002-9378(95)90563-4

Cited by 26 publications

(12 citation statements)

References 21 publications

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“…The apparently lower qualitative uptake of the silica NPs in the perfused placental tissue compared to the BeWo cells can be explained by the differences in exposure on the basis of mass per surface area. The total exchange area of a term human placenta is approximately 13 m 2 (Larsen et al 1995; Syme et al 2004), but the total exchange area in a single Transwell ® insert containing BeWo cells is 1.12 cm 2 . The average weight of the placentas used in these perfusion experiments was 773 g, and the average weight of the perfused cotyledons was 25 g. Even though the NP concentration in both systems was 100 μg/mL, the volume in the donor (maternal) compartment is 100 mL in the perfusion experiments, but only 0.5 mL in the BeWo cell studies.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of silica nanoparticles in the human placenta

et al. 2013

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The potential medical applications of nanoparticles warrant their investigation in terms of biodistribution and safety during pregnancy. The transport of silica nanoparticles (NPs) across the placenta was investigated using two models of maternal-fetal transfer in human placenta, namely, the BeWo b30 choriocarcinoma cell line and the ex vivo perfused human placenta. Nanotoxicity in BeWo cells was examined by the MTT assay and demonstrated decreased cell viability at concentrations greater than 100 μg/mL. In the placental perfusion experiments, antipyrine crossed the placenta rapidly, with a fetal/maternal ratio of 0.97 ± 0.10 after 2 hours. In contrast, the percentage of silica NPs reaching the fetal perfusate after 6 hours was limited to 4.2 ± 4.9% and 4.6 ± 2.4% for 25-nm and 50-nm NPs, respectively. The transport of silica NPs across the BeWo cells was also limited, with an apparent permeability of only 1.54 × 10−6 ± 1.56 × 10−6 cm/sec. Using confocal microscopy, there was visual confirmation of particle accumulation in both BeWo cells and in perfused placental tissue. Despite the low transfer of silica NPs to the fetal compartment, questions regarding biocompatibility could limit the application of unmodified silica NPs in biomedical imaging or therapy.

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Section: Discussionmentioning

confidence: 99%

Kinetics of silica nanoparticles in the human placenta

et al. 2013

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“…The most characteristic histological abnormality, found in a proportion of cases but certainly not in all, is decreased fetal perfusion of the placental villi 13. The fetal villous vessels are normal in placentas from prolonged pregnancies44 and Doppler flow velocimetry studies have, in general but not unanimously, indicated that there is no increased fetal vascular resistance in such placentas 45-47. The decreased fetal perfusion is therefore probably a consequence of oligohydramnios, because umbilical vein flow studies have shown that fetal blood flow to the placenta is often reduced in cases of oligohydramnios 48…”

Section: Clinical Factorsmentioning

confidence: 99%

Aging of the placenta

Fox

1997

Archives of Disease in Childhood - Fetal and Neonatal Edition

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“…We speculate that possible changes in the placental vascular bed are not reflected into these rather large caliber arteries or that the pathophysiology of placental malfunction in prolonged pregnancies is based on other mechanisms. Fujikura reported decreased placental villous infiltration with advancing gestation . However, on a stereologic examination, the trophoblast volume and villous capillary volume did not differ between term and post‐term placentas …”

Section: Discussionmentioning

confidence: 93%