A stereological study of the renal and adrenal glandular structure of red-legged partridge (Alectoris chukar)

Abstract

Background: Partridge is a major bird species that has recently gained an increasing importance as an alternative food source and a game animal in hunting tourism. Herein, we aimed to examine the histological structure and stereological properties of the renal and adrenal glands of red-legged partridge. Materials and methods: It was used 7 healthy adult red-legged partridges (Alectoris chukar) of both genders in this study. The Alectoris chukars were perfused with the intracardiac method and were kept in 10% formaldehyde for 72 h for optimal fixation. The tissues were embedded in paraffin after routine light microscopic processes. Then, 5-µm thick sections were taken, which were stained with Hematoxylin & Eosin, photographed, and examined in light microscope. Modified Cavalieri principle was used for volume calculation as stereological analysis. Total tissue volume ratios were calculated with the help of a point grid provided by the Shtereom 1.5 packaged program. Mann Whitney-U test was used for intergroup comparisons. The significance level was set at 5% SPSS (ver: 13) statistical software was used for all statistical analyses. Results: Renal histological structure was found to resemble to those of other bird species and contained a mixed type (mammalian, reptilian) glomerular structure. It was also found that the adrenal histology was not in the form of separate layers but composed of cell groups with different properties. Stereological renal volume assessment revealed statistically similar right and left renal size (p>0.05). In adrenal gland volume assessment, the right and left adrenal volumes were also statistically similar (p>0.05). Conclusions: The obtained data in the present study is thought contribute to the understand of the stereological, morphological and histological structure of the red-legged partridge (Alectoris chukar) kidney and adrenal gland.

Morphological changes in the adrenal glands of chickens under heat stress

“…Подібно до курчат контрольної групи -у курчат дослідних груп органометричні показники правої надниркової залози превалювали над такими лівої залози (табл.). Проте окремі автори (Sarkar et al, 2014;Colcimen & Cakmak, 2020;Prokopenko & Kot, 2021;Jabbar et al, 2021) стверджують, що у птахів ліва надниркова залоза, порівняно з правою, має більші показники маси, об'єму і довжини, що є результатом інтенсивної їх васкуляризації. Маса і розміри надниркової залози птахів змінюються в період їх статевої активності, порушенні умов утримання, дії фармакологічних препаратів, дії стресфактору тощо (Muller et al, 2015).…”

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“…The latter method is characterized by high accuracy for assessing the structural and functional state of animals at the levels of organs, tissues, and cells in normal and pathological conditions (Bancroft & Gamble, 2007). Scientific sources mainly present information on organometry of the adrenal gland of birds (Kober et al, 2012;Colcimen & Cakmak, 2020;. Information on the morphometry of microstructural components of the adrenal glands in birds is limited.…”