A Stereological Study of the Toxic Effects of Cerium Oxide during Pregnancy on Kidney Tissues in Neonatal NMRI Mice

Nemati, Afsaneh; Assadollahi, Vahideh; Peluso, Ilaria; Abbaszadeh, Abolfazl; Boroujeni, Mandana Beigi; Khanipur, Zahra; Gholami, Mohammadreza

doi:10.1155/2020/9132724

Cited by 8 publications

(5 citation statements)

References 59 publications

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“…In some metal nanoparticles such as silver, gold, and copper, the toxicity increases with decreasing nanoparticle size [27]. Other physicochemical properties such as elemental composition, surface charge, shape, crystallinity, solubility also affect the toxic potential of the compounds [11,12]. Therefore, metal-based nanoparticles should not be considered a homogeneous population with basic toxic properties since they act individually to elicit various biological reactions.…”

Section: Discussionmentioning

confidence: 99%

“…For MTT assay, 104 cells/well were cultured in a 96-well plate (Thermo Fisher Scientific) and incubated overnight at 37°C with 5% CO 2 . According to previous studies, 100µL of different concentrations of CeNPs (15.625, 31.25, 62.5, 125, 250, & 500 ug/mL) were added to each well in triplicates, and cells were incubated for 24 h at 37°C [11]. Then, one hundred µL (0.5 mg/mL) of MTT (Sigma-Aldrich, Germany) was added to each well and incubated for four h at 37°C.…”

Section: In Vitro Toxicity Assay Of the Synthesized Cenpsmentioning

confidence: 99%

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Preparation of Cerium Oxide Nanoparticles and Their Cytotoxicity Evaluation In vitro and In vivo

Zandi

Fazeli²,

Bigdeli³

et al. 2021

IJMTFM

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Background: Nanotechnology plays a significant role in medicine, especially in diagnosis and treatment as a carrier to drugs and vaccinology. Several studies were conducted using nanoparticles as an adjuvant. The main aim of this study was in vivo and in vitro toxicity evaluation of synthesized Cerium Nanoparticles (CeNPs). Methods: In the present study, cerium nanoparticles were prepared using the wet chemical method. The formation of cerium nanoparticles was confirmed by scanning electron microscopy, transmission electron microscopes, x-ray diffraction analysis, dynamic light scattering. In vivo and in vitro toxicity of synthesized nanoparticles was evaluated in three different amounts of cerium nanoparticles (30 μg, 50 μg, & 100 μg) in mice and human fibroblast cell lines, respectively. Results: Cerium nanoparticles were successfully synthesized, and the identity was confirmed by x-ray diffraction analysis. The shape and size of nanoparticles were spherical and <100 nm, respectively. The prepared nanoparticles had a charge of -26.6 mV and a hydrodynamic radius of 446 nm. MTT assay indicated that none of the concentration of cerium was toxic, and in vivo toxicity also clarified the safety of cerium nanoparticles in mice; no significant un-normal behavioral and physical symptoms were observed in mice after CeNP administration Conclusion: Cerium nanoparticles have special properties, especially low toxicity, unique capabilities in stimulating the immune system. Cerium nanoparticles can be considered an effective and safe candidate in vaccines.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Toxicity Assay Of the Synthesized Cenpsmentioning

confidence: 99%

Preparation of Cerium Oxide Nanoparticles and Their Cytotoxicity Evaluation In vitro and In vivo

Zandi

Fazeli²,

Bigdeli³

et al. 2021

IJMTFM

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“…A higher ratio of Ce 3+ / Ce 4+ reduces the reactive oxygen species, with the antioxidant properties of Ce depending on its ability to deform and rotate between the two states. Thus, it can be stated that the toxic effects of CeO 2 are dose and time‐dependent, depending on the cell and tissue (Das, Choi, Han, Reza, & Kim, 2017; Nemati et al, 2020; Nemati, Farhadi, Jalili, & Gholami, 2020; Rogers, 2013).…”

Section: Discussionmentioning

confidence: 99%

The effect of different concentrations of cerium oxide during pregnancy on ovarian follicle development in neonatal mice

Nemati

Beyranvand

Assadollahi

et al. 2020

Birth Defects Research

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Objectives Cerium is a member of the rare metals group and widely used in drug delivery, gene therapy, molecular imaging and medicine. In this study, we investigated the effect of different doses of Cerium (IV) oxide (CeO2) during pregnancy on neonatal mice ovaries, as well as its effect on blood biochemical parameters. Methods Thirty pregnant NMRI mice were divided into five groups: Control and 4 groups treated with CeO2 (10, 25, 80, 250 mg/kg.bw i.p) at the GD7 and GD14. The ovarian histological of neonatal (2 and 6 day‐olds), as well as blood serum of neonates at 15‐dpp were analyzed. Results Count of ovarian primordial follicles in neonates at 2 dpp showed a significant decrease in the groups treated with 80 and 250 mg/kg.bw doses of CeO2. There was also a significant decrease in ovarian primordial and primary follicles in neonates at 6‐dpp at 250 mg/kg.bw doses of CeO2 in the control (P < 0.05). There was no significant difference in serum levels of malondialdehyde and total antioxidant capacity between the experimental and control groups. Conclusions Our results suggest that the effects of CeO2 on the ovarian tissue of neonatal mice during pregnancy may be dose‐dependent.

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“…Oral exposure during pregnancy would therefore be safer compared to intravenous injection or pulmonary exposure, with a low passage of nanoceria through the intestinal barrier as described previously, and most CeO 2 NPs are excreted in feces. Studies on the intraperitoneal administration of CeO 2 particles larger than NPs (<5 µm) to pregnant mice found dose-dependent defects in fetal renal development, which results in kidney damage [126] and alterations in the testicular tissue development of the pups [127].…”

Section: Ceo 2 and Pregnancymentioning

confidence: 99%

On Placental Toxicology Studies and Cerium Dioxide Nanoparticles

Deval

Boland

Fournier

et al. 2021

IJMS

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The human placenta is a transient organ essential for pregnancy maintenance, fetal development and growth. It has several functions, including that of a selective barrier against pathogens and xenobiotics from maternal blood. However, some pollutants can accumulate in the placenta or pass through with possible repercussions on pregnancy outcomes. Cerium dioxide nanoparticles (CeO2 NPs), also termed nanoceria, are an emerging pollutant whose impact on pregnancy is starting to be defined. CeO2 NPs are already used in different fields for industrial and commercial applications and have even been proposed for some biomedical applications. Since 2010, nanoceria have been subject to priority monitoring by the Organization for Economic Co-operation and Development in order to assess their toxicity. This review aims to summarize the current methods and models used for toxicology studies on the placental barrier, from the basic ones to the very latest, as well as to overview the most recent knowledge of the impact of CeO2 NPs on human health, and more specifically during the sensitive window of pregnancy. Further research is needed to highlight the relationship between environmental exposure to CeO2 and placental dysfunction with its implications for pregnancy outcome.

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A Stereological Study of the Toxic Effects of Cerium Oxide during Pregnancy on Kidney Tissues in Neonatal NMRI Mice

Cited by 8 publications

References 59 publications

Preparation of Cerium Oxide Nanoparticles and Their Cytotoxicity Evaluation In vitro and In vivo

Preparation of Cerium Oxide Nanoparticles and Their Cytotoxicity Evaluation In vitro and In vivo

The effect of different concentrations of cerium oxide during pregnancy on ovarian follicle development in neonatal mice

On Placental Toxicology Studies and Cerium Dioxide Nanoparticles

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