A Steroid Growth Factor Requirement in a Fungus

Elliott, C. G.; Hendrie, M. E.; Knights, B.A.; Parker, W.

doi:10.1038/203427b0

Cited by 63 publications

(21 citation statements)

References 8 publications

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“…Sterols do not appear to be essential for vegetative growth, which can take place in sterol-free medium, although these fungi appear not to synthesize them (Elliott et al 1964;Hendrix, 1966;Schlosser & Gottlieb, 1966). The role of sterols in their metabolism can thus be studied simply by adding the sterol to the medium.…”

Section: Introductionmentioning

confidence: 99%

“…Sterols have been shown to be required for sexual reproduction in the oomycetes (Hendrix, 1964;Haskins, Tulloch & Micetich, 1964;Elliott, Hendrie, Knights & Parker, 1964;Leal, Friend & Holliday, 1964), and they are also required for the production of normal sporangia and zoospores (Chee & Turner, 1965;Hendrix, 1965). Sterols do not appear to be essential for vegetative growth, which can take place in sterol-free medium, although these fungi appear not to synthesize them (Elliott et al 1964;Hendrix, 1966;Schlosser & Gottlieb, 1966).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Inhibitors of Sterol Synthesis on Growth of Sordaria and Phytophthora

Elliott

1969

Journal of General Microbiology

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SUMMARYFour hypocholesteremic compounds, SKF 330I-A, SKF 525-A, SKF 16467-A and AY 9944, inhibited the growth of SordariaJimicola; SKF 2314, SKF 7732-A3 and SKF 7997-A3 did not. All seven compounds inhibited the growth of Phytophthora cactorum. The inhibition of growth of S. Jimicola by SKF 3301-A and AY 9944 was annulled by certain unsaturated fatty acids but not by sterols; with P. cactorum the inhibition was annulled by oleic acid + cholesterol.With Sordaria Jimicola sub-inhibitory concentrations of SKF 3301-A caused growth which showed two phases, distinguished by an abrupt change in growth rate. At the position of the hyphal front when growth rate changed a ring of perithecia was formed. During the second period, when growth was slower, perithecia were formed in rings: The number of perithecia formed could be at least twice that in the drug-free controls. With AY 9944 no perithecia were produced at concentrations sub-inhibitory to vegetative growth. On media with SKF 3301-A or AY 9944 and oleic acid, few perithecia were formed despite good vegetative growth; with SKF ~~o I -A , but not AY 9944, the addition of cholesterol as well increased perithecial production.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Inhibitors of Sterol Synthesis on Growth of Sordaria and Phytophthora

Elliott

1969

Journal of General Microbiology

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“…For over two decades it has been accepted that sterolauxotrophic fungi of the Pythiaceae (Oomycetes) require an exogenous source of these compounds for sexual reproduction (14,15,(19)(20)(21). Requirements for sterols have also been documented for asexual (11) and sexual (30) reproduction by the facultative mosquito parasite Lagenidiim ,ngiganteuim (Oomycetes:Lagenidiales), which cannot synthesize these compounds (60).…”

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confidence: 99%

Reassessment of the role of phospholipids in sexual reproduction by sterol-auxotrophic fungi

Kerwin

Duddles

1989

J Bacteriol

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Several genera of oomycete fungi which are incapable of de novo sterol synthesis do not require these compounds for vegetative growth. The requirement for an exogenous source of sterols for sexual reproduction by several members of the Pythiaceae has been questioned by reports of apparent induction and maturation of oospores on defined media supplemented with phospholipids in the absence of sterols. A more detailed examination of this phenomenon suggested that trace levels of sterols in the inoculum of some pythiaceous fungi act synergistically with phospholipid medium supplements containing unsaturated fatty acid moieties to induce oosporogenesis. Phospholipid analysis of one species, Pythium ultimum, suggested that only the fatty acid portion of the exogenous phospholipid is taken up by the fungus. Enrichment of the phospholipid fraction of total cell lipid of P. ultimum with unsaturated fatty acids promoted oospore induction, and enhanced levels of unsaturated fatty acids in the neutral lipid fraction increased oospore viability. For some pythiaceous fungi, the levels of sterols required for the maturation of oospores with appropriate phospholipid medium supplementation suggest that these compounds are necessary only for the sparking and critical domain roles previously described in other fungi.

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“…We previously reported the occurrence of oospores in small numbers in cultures grown on basal medium, and with some substances now classified as inactive and partially active (Elliott et al 1964). This was due to the use at that time of Oxoid agar No.…”

Section: An Effect Of Agarmentioning

confidence: 97%

“…However, in media to which certain sterols are added, vegetative growth is increased and, more significantly, sexual organs (oogonia and antheridia) develop and oospores are formed (Haskins, Tulloch & Micetich, 1964;Hendrix, 1964Hendrix, , 1965Elliott, Hendrie, Knights & Parker, 1964;Leal, Friend & Holliday, 1964;Harnish, Berg & Lilly, 1964). In this paper we consider what structural features of the sterol molecule are necessary t o confer this physiological activity on it in the case of Phytophthora cactorum (Leb.…”

Section: Introductionmentioning

confidence: 99%

The Sterol Requirement of Phytophthora Cactorum

Elliott¹,

Hendrie²,

Knights³

1966

Journal of General Microbiology

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SUMMARYSeveral sterols and related substances were tested for ability to promote sexual reproduction in Phytophthora cactorum. These compounds were classified as active (oospores formed), partially active (oogonia and antheridia formed, but no oospores), or inactive (no oogonia). Quantitative differences between active substances were noted in respect of the numbers of oospores produced and the time of their appearance. The most active substances tested were 29-isofucosterol and p-sitosterol. Loss of activity was associated with shortening of the side chain of the sterol molecule or change in its configuration, absence of a double bond in the B ring or of a hydroxyl group a t position 3 on the A ring.

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A Steroid Growth Factor Requirement in a Fungus

Cited by 63 publications

References 8 publications

Effects of Inhibitors of Sterol Synthesis on Growth of Sordaria and Phytophthora

Effects of Inhibitors of Sterol Synthesis on Growth of Sordaria and Phytophthora

Reassessment of the role of phospholipids in sexual reproduction by sterol-auxotrophic fungi

The Sterol Requirement of Phytophthora Cactorum

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