A sticky-end probe biosensor for homogeneous detection of transcription factor binding activity

Nanoquantification of RUNX2 by a 1,1′-carbonyldiimidazole-diamond mediated sandwich assay for osteogenic differentiation

“…For instance, a single-molecule DNA-based biosensor was developed to identify transcription factors by colocalizing the two halves of the binding sites with the attached single molecules of a two-color DNA-based biosensor, achieving detection as low as 150 pM [ 37 ]. In another study, the transcription factor SOX9 was detected using the sticky end biosensor method to monitor transcription factor and DNA binding activity, helping to monitor live cell activity [ 38 ]. In our sandwich assay, the transcription factor RUNX2 was successfully detected as low as 0.1 ng/mL, providing valuable insights for monitoring the condition of osteogenic differentiation.…”