A strain of Bacillus thuringiensis subsp. galleriae containing a novel cry8 gene highly toxic to Anomala cuprea (Coleoptera: Scarabaeidae)

Asano, Sumitada; Yamashita, Chikage; Iizuka, Toshihiko; Takeuchi, Katsuyoshi; Yamanaka, Satoshi; Cerf, David C.; Yamamoto, Takashi

doi:10.1016/s1049-9644(03)00060-4

Cited by 51 publications

(33 citation statements)

References 13 publications

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“…The protein matches the molecular mass calculated for other Cry8 proteins reported previously [Asano et al, 2003;Shu et al, 2009] figure 2 . Cry8 INTA Fr7-4 contained the five blocks (1-5) characteristic of other Cry proteins and the three blocks (6-8) present in toxins that are cleaved in the C-terminal region [Schnepf et al, 1998].…”

Section: Sequence Analysis Of Cry8 Inta Fr7-4 Proteinsupporting

confidence: 86%

Identification, Cloning and Expression of an Insecticide cry8 Gene from Bacillusthuringiensis INTA Fr7-4

Amadío

Navas²,

Sauka³

et al. 2013

Microb Physiol

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Insecticidal activity of Bacillus thuringiensis is attributed largely to the crystal proteins. These were found with specific toxic activity against insects in different orders. A novel cry8 gene from B. thuringiensis strain INTA Fr7-4 from Argentina was characterized. The encoded protein, Cry8Qa2, is 1184 amino acids long and its sequence is more similar to those of Cry8F class. We cloned and expressed the protein in an acrystalliferous strain of B. thuringiensis using two differentially regulated promoters. The recombinant strains produced ovoid crystals with low toxicity against larvae of Anticarsia gemmatalis (Lepidoptera: Noctuidae). The morphology and insecticidal properties of these crystals resembled those produced by the native strain INTA Fr7-4.

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Section: Sequence Analysis Of Cry8 Inta Fr7-4 Proteinsupporting

confidence: 86%

Identification, Cloning and Expression of an Insecticide cry8 Gene from Bacillusthuringiensis INTA Fr7-4

Amadío

Navas²,

Sauka³

et al. 2013

Microb Physiol

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“…Cry8-type toxins have been described as toxic to coleopteran pests (mainly members of the Scarabaeidae family) [Asano et al, 2003;Shu et al, 2009], and to some lepidopteran pests [Amadio et al, 2013]. To our knowledge, there was only one work where Cry8 proteins, Cry8Ka in particular, showed toxicity against A. grandis larvae [Oliveira et al, 2011].…”

Section: Insect Bioassays Of Wild-type and Recombinant Cry8 Proteinsmentioning

confidence: 99%

“…Anomala cuprea [Asano et al, 2003], Holotrichia parallela [Shu et al, 2009], Anthonomus grandis [Oliveira et al, 2011], and also to some lepidopteran pests, e.g. Anticarsia gemmatalis [Amadio et al, 2013].…”

Section: Introductionmentioning

confidence: 99%

Sequence and Expression of Two cry8 Genes from Bacillus thuringiensis INTA Fr7-4, a Native Strain from Argentina

Navas

Berretta²,

Pérez³

et al. 2014

Microb Physiol

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We found and characterized two cry8 genes from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. These genes, cry8Kb3 and cry8Pa3, are located in a tandem array within a 13,200-bp DNA segment sequenced from a preparation of total DNA. They encode 1,169- and 1,176-amino-acid proteins, respectively. Both genes were cloned with their promoter sequences and the proteins were expressed separately in an acrystalliferous strain of B. thuringiensis leading to the formation of ovoid crystals in the recombinant strains. The toxicity against larvae of Anthonomus grandis Bh. (Coleoptera: Curculionidae) of a spore-crystal suspension from the recombinant strain containing cry8Pa3 was similar to that of the parent strain INTA Fr7-4.

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“…Since the Agrobacterium mediated transformation system does not involve sophisticated equipment and frequently produces cleaner events (intact integrations and single copy) than other methods [13], it remains to be the more favorable approach. Previous studies on sweet potato transformation via Agrobacterium tumefaciens transformation have been reported including herbicide resistance [14,15], viral resistance [16], insect resistances related to the CryIIIA delta-endotoxin, cowpea trypsin inhibitor and snowdrop lectin [17,18] and enhancing starch accumulation in tubers [19,20]. To date, effective transformation protocols have been developed for only few of sweet potato cultivars, indicating that it is a very recalcitrant species and transformation methods of sweet potato are still genotype dependent.…”

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confidence: 99%

Agrobacterium-Mediated Transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

Ngoc¹,

Lan²,

Trang³

et al. 2015

JLS

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Sweet potato [Ipomoea batatas (L.) Lam.] is an important food crop in the world as well as in Vietnam. It is well known as a recalcitrant crop for gene transformation and tissue culture because of its genotype dependent in vitro responses. In present study, Agrobacterium-mediated transformation of cry8Db from Bacillus thuringiensis into KB1 sweet potato variety has been studied. The C58cv strain carrying a pBI121 backbone which contained cry8Db delta-endotoxin gene regulated under 35S CaMV promoter, and the selection marker gene, neomycin phosphotransferase (nptII) gene, was subjected for plant transformation. Callus induced from shoot tips and leaf explants were inoculated and cocultured with A. tumefaciens. The selection occurred during callus producing and plant regenerating steps. A total of 201 transgenic putative plant lines were produced, and 21 transgenic lines were positively confirmed by PCR and finalized by Southern blot. Four putative transgenic lines confirming a single copy of the cry8Db gene were transferred into soil pots in greenhouse. Biological activity evaluation for the insecticidal capacity of these transgenic lines under controlled conditions showed that the level of infestation by sweet potato weevils (Cylas formicarius) in untransformed plants was higher than that of transgenic lines.

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A strain of Bacillus thuringiensis subsp. galleriae containing a novel cry8 gene highly toxic to Anomala cuprea (Coleoptera: Scarabaeidae)

Cited by 51 publications

References 13 publications

Identification, Cloning and Expression of an Insecticide <b><i>cry8</i></b> Gene from <b><i>Bacillus</i></b><b><i>thuringiensis </i></b>INTA Fr7-4

Identification, Cloning and Expression of an Insecticide <b><i>cry8</i></b> Gene from <b><i>Bacillus</i></b><b><i>thuringiensis </i></b>INTA Fr7-4

Sequence and Expression of Two <b><i>cry8</i></b> Genes from <b><i>Bacillus thuringiensis</i></b> INTA Fr7-4, a Native Strain from Argentina

Agrobacterium-Mediated Transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

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