A Strain of Pseudomonas sp. Isolated from Piggery Wastewater Treatment Systems with Heterotrophic Nitrification Capability in Taiwan

Abstract

A high concentration of NH(4) (+) in piggery wastewater is major problem in Taiwan. Therefore, in our study, we isolated native heterotrophic nitrifiers for piggery wastewater treatment. Heterotrophic nitrifier AS-1 was isolated and characterized from the activated sludge of a piggery wastewater system. Sets of triplicate crimp-sealed serum bottles were used to demonstrate the heterotrophic nitrifying capability of strain AS-1 in an incubator at 30 degrees C. All serum bottles contained 80 mL medium, and the remainder of the bottle headspace was filled with pure oxygen. The experimental results showed that 2.5 +/- 0.2 mmol L(-1) NH(4) (+) was removed by 58 hours, and, eventually, 1.5 +/- 0.5 mmol L(-1) N(2) and 0.2 +/- 0.0 mmol L(-1) N(2)O were produced. The removal rate of NH(4) (+) by the strain AS-1 was 1.75 mmol NH(4) (+) g cell(-1) h(-1). This strain was then identified as Pseudomonas alcaligenes (97% identity) by sequencing its 16S rDNA and comparing it with other microorganisms. Thus, strain AS-1 displays high promise for future application for in situ NH(4) (+) removal from piggery wastewater.

Agricultural use of treated sewage effluents: agronomic and environmental implications and perspectives for Brazil

“…On the other hand, the addition of organic compounds by high inputs of soluble effluent-C favours the heterotrophic microbial communities generating NO 3 -by heterotrophic nitrification (Nurk et al, 2005;Su et al, 2006). These microbial communities are also capable to accomplish denitrification using NO 3 -and NO 2 -(nitrite) as electron acceptors resulting in the formation of nitrous oxide (N 2 O) (McLain & Martens, 2006).…”

Heterotrophic Nitrification and Aerobic Denitrification by Four Novel Isolated Bacteria

“…Traditionally, heterotrophic nitrifying strains were usually obtained from wastewater [2,10] or membrane bioreactors [3,7]. In our works, various environment samples, such as soil, sludge, and water, were screened for microorganisms with heterotrophic nitrification ability.…”

“…Pseudomonas sp. was a very common genus with the ability of heterotrophic nitrification [2,10]. The genus could be classified into three groups based on their modes of denitrification [6].…”

“…Similarly, another Pseudomonas sp. strain was isolated from piggery wastewaters treatment system which removed 35 mg/L NH 4 + -N with few NO 2 -N accumulation [10].…”

Heterotrophic Nitrification-Aerobic Denitrification Performance of Strain Y-12 under Low Temperature and High Concentration of Inorganic Nitrogen Conditions

“…Although some bacteria could remove NH 4 + -N and NO 3 − -N (or NO 2 − -N), respectively [16,17], few bacteria could remove NH 4 + -N and NO 3 − -N (or NO 2 − -N) simultaneously [18]. In addition, environmental factors, such as high-concentration substrates and low temperature, could inhibit the cell growth and influence the removal efficiency [14,[19][20][21]. Therefore, screening and studying more efficient microbes, which were capable of removing sole and mixed nitrogen sources under conditions of low temperature and high-concentration substrates, have become an important task in the technology of biological denitrification.…”