A Strategy for Sequencing Based on Rolling-Circle Amplification on a Microarray

Gao, Li; Cui, Jingjie; Zhou, Yang; Chen, Liang; Zhang, Chunxia; Chen, Keping; Lian, Chaoqun; Li, Lihua; Li, Hong; Liang, Jinke

doi:10.1166/jbn.2013.1576

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…Unlike traditional DNA amplification techniques, such as the PCR system, RCA is an isothermal nucleic acid amplification technique in which the polymerase continuously adds nucleotides to a circular template, resulting in a long tandem repeat (ssDNA) containing tens to hundreds of tandem repeats (complementary to the circular template) [19]. The DNA polymerases used in RCA are phi29, Bst, and Vent (exo-), but since phi29 DNA polymerase has the best processivity and strand displacement ability among the above polymerases, it is commonly used in the RCA reaction process [20][21][22]. Unlike PCR systems, RCA can be performed at a constant temperature (from room temperature to 37 • C) [23].…”

Section: Determination Of the Spontaneous Mutation Rate Of Rpsl In Th...mentioning

confidence: 99%

A Genetic Toxicology Study of the Rapid Detection of Nitrosamine Compounds by the rpsL Gene Mutation Assay

Peng

Zhang

Liu

2022

Foods

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In a rpsL gene mutation experiment, the mutagenicity of the nitrosamine compounds N-diethylnitrosamine (NDEA) and N-dipropylnitrosamine (NDPA) was investigated at the cellular level, as well as with PCR (polymerase chain reaction) and RCA (rolling-circle amplification) amplification systems. The experiments were set up with 10 ppm, 100 ppm, and 1000 ppm concentration gradients of NDEA and NDPA, and ethidium bromide (EB) was used as a positive control group. The results demonstrated that the mutagenic frequency of NDEA and NDPA was significantly higher than the spontaneous mutation frequency of the rpsL gene under the same conditions, but lower than the mutagenic rate of EB in the positive control, and there was a dose-effect relationship, indicating that NDEA and NDPA could induce rpsL gene mutation. The rpsL mutation system has a low spontaneous mutation background and high sensitivity, thus the system is expected to become an effective tool for the rapid detection of carcinogens in the field of food.

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Section: Determination Of the Spontaneous Mutation Rate Of Rpsl In Th...mentioning

confidence: 99%

A Genetic Toxicology Study of the Rapid Detection of Nitrosamine Compounds by the rpsL Gene Mutation Assay

Peng

Zhang

Liu

2022

Foods

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“…These advantages make it possible to use RCA during the war, large epidemics and so on. RCA is normally available to amplify template (circularized single DNA) sequence when the primer complemented with the template is also DNA [14]. Compared with real-time PCR, RCA avoids the separation step of reverse transcription of miRNA and allows for the direct and rapid detection of miRNAs in a shorter period of time.…”

Section: Introductionmentioning

confidence: 99%

Discriminative identification of miRNA let-7 family members with high specificity and sensitivity using rolling circle amplification

Zhao

Song

Guan

2015

ABBS

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Rolling circle amplification (RCA) is a new method based on virus DNA reproduction, which has been widely used in the field of miRNA detection. However, discrimination of highly homologous miRNAs is a bottleneck in the research of miRNA. In this study, the RCA process was creatively used to conduct the discrimination of miRNAs. Results showed that T4 RNA ligase 2 could reach the highest circularization efficiency during the RCA process with higher specificity. By using RCA technology, a member of highly homologous miRNAs, let-7, could be discriminated at the amount of 2.5 fmol. This sensitivity could not be achieved by using traditional reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. In addition, detection of miRNAs by using RCA could reach the amount limit of fmol with a good linearity. Optimal RCA technology used in this study is better than RT-qPCR in discriminating highly homologous family miRNAs. Results from this study can promote the applications of RCA in clinical diagnosis, environment protection, health care, disease inspection and prevention, and national security.

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Protein detection based on rolling circle amplification sensors

Shi

Cui

Sulemana³

et al. 2021

Luminescence

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Rolling circle amplification (RCA) is an isothermal process under the action of DNA polymerases. Large-scale DNA templates have been generated using RCA for target detection. Some signal amplification strategies including optical sensors and electrochemical sensors based on RCA have been applied to achieve sensitive detection.Sensors based on RCA have attracted increasing interest. Advances in RCA-based sensors for protein detection are reviewed in this paper. The advantages and detection mechanisms of sensors based on RCA are revealed and discussed. Finally, possible challenges and future perspectives are also outlined.

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A Strategy for Sequencing Based on Rolling-Circle Amplification on a Microarray

Cited by 5 publications

References 31 publications

A Genetic Toxicology Study of the Rapid Detection of Nitrosamine Compounds by the rpsL Gene Mutation Assay

A Genetic Toxicology Study of the Rapid Detection of Nitrosamine Compounds by the rpsL Gene Mutation Assay

Discriminative identification of miRNA let-7 family members with high specificity and sensitivity using rolling circle amplification

Protein detection based on rolling circle amplification sensors

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