A strategy for soluble overexpression and biochemical characterization of halo-thermotolerant Bacillus laccase in modified E. coli

Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

“…These enzymes catalyze the monoelectronic oxidation of a wide number of phenolic compounds and aromatic amines using oxygen as a nonlimited electron acceptor and their substrate range is expanded to non phenolic aromatic compounds in the presence of low-molecular-mass compounds, which acts as redox mediators [4,7,8]. Fungal laccases are glycoproteins with monomeric, dimeric or tetrameric structure, containing four redox-active copper atoms that are distributed in three distinct types (T1, T2 and T3) based on their spectroscopic properties [3].…”

“…During last years a wide number of studies have focused on lignolytic fungi because they have a versatile enzyme system (including laccases, peroxidases and oxidases producing H2O2) which participate in the degradation of lignin but also of other recalcitrant aromatic compounds causing environmental problems [2]. Among these groups of extracellular enzymes, laccases (benzenediol: oxygen oxidoreductases, EC 1.10.3.2) have received great attention because they possess huge applicability in different biotechnological and industrial applications [3,4,5], such…”

Heterologous expression of an acidophilic multicopper oxidase in Escherichia coli and its applications in biorecovery of gold

“…As shown in Table 2, the reaction conditions of MCO activity are very diverse among different microbes (Ye et al 2010;Ausec et al 2015;Yang et al 2016;Safary et al 2016). However, rare forms of MCO show acidophilic properties and accordingly our results of P. hauseri MCO had optimal activity at pH 2.2.…”

Identification and Molecular Characterization of Genes Coding Pharmaceutically Important Enzymes from Halo-Thermo Tolerant Bacillus

“…Since, the most industrial processes are often performed in harsh conditions like extreme pH, temperature, or ionic strength, therefore such robust enzymes provide economically appealing materials to be used under specific physicochemical situations. 27,66 Thus, halo-thermo tolerance Bacillus sp. SL-1 could represent an excellent source of enzymes that can function at extreme conditions working as biocatalysts in medical, pharmaceutical, chemical and other industries.…”

“…SL-1 revealed its high production yield and stability. 27 Aldehyde dehydrogenase (ALDH; aldehyde: NAD(P) + oxidoreductase, EC 1.2.1.5) constitute a group of enzymes that catalyze the conversion of aldehydes to the corresponding acids mediated by an NAD(P) + -dependent virtually irreversible reaction making it potentially useful in an industrial settings. The ALDH is very unstable because of the spontaneous oxidation, 34 therefore, there is considerable interest in production of stable ALDH, which can be used more efficiently in the pharmaceutical and fine chemicals industries for the production of aldehydes, ketones, and chiral alcohols.…”