A Stress Surveillance System Based on Calcium and Nitric Oxide in Marine Diatoms

Vardi, Assaf; Formiggini, Fabio; Casotti, Raffaella; Martino, Alessandra De; Ribalet, François; Miralto, Antonio; Bowler, Chris

doi:10.1371/journal.pbio.0040060

Cited by 267 publications

(380 citation statements)

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“…The importance of calcium (Ca 2ϩ )-based second messenger signaling pathways in P. tricornutum has been shown for nitric oxide (NO)-mediated death and defense responses and for cellular perception of bioavailable Fe concentrations (31,32). Diatom AOX proteins contain calcium-binding EF-hand domains.…”

Section: Resultsmentioning

confidence: 99%

Whole-cell response of the pennate diatom Phaeodactylum tricornutum to iron starvation

Allen

LaRoche²,

Maheswari

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Marine primary productivity is iron (Fe)-limited in vast regions of the contemporary oceans, most notably the high nutrient low chlorophyll (HNLC) regions. Diatoms often form large blooms upon the relief of Fe limitation in HNLC regions despite their prebloom low cell density. Although Fe plays an important role in controlling diatom distribution, the mechanisms of Fe uptake and adaptation to low iron availability are largely unknown. Through a combination of nontargeted transcriptomic and metabolomic approaches, we have explored the biochemical strategies preferred by Phaeodactylum tricornutum at growth-limiting levels of dissolved Fe. Processes carried out by components rich in Fe, such as photosynthesis, mitochondrial electron transport, and nitrate assimilation, were down-regulated. Our results show that this retrenchment is compensated by nitrogen (N) and carbon (C) reallocation from protein and carbohydrate degradation, adaptations to chlorophyll biosynthesis and pigment metabolism, removal of excess electrons by mitochondrial alternative oxidase (AOX) and non-photochemical quenching (NPQ), and augmented Fe-independent oxidative stress responses. Iron limitation leads to the elevated expression of at least three gene clusters absent from the Thalassiosira pseudonana genome that encode for components of iron capture and uptake mechanisms.genome ͉ metabalomics ͉ photosynthesis ͉ transcriptomics ͉ nutrients

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Section: Resultsmentioning

confidence: 99%

Whole-cell response of the pennate diatom Phaeodactylum tricornutum to iron starvation

Allen

LaRoche²,

Maheswari

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

406

505

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“…Similarly, our results also showed that 6-chloroindole increased Ca 2+ efflux in algal cells. It was reported that calcium ion was closely related to algal many physiological activities and the adhesion of diatom also required the involvement of calcium (Cooksey and Cooksey, 1992;Vardi et al, 2006). Therefore, Ca 2+ efflux induced by 6-chloroindole might promote the inhibitory effect on the growth and adhesion of diatom.…”

Section: Discussionmentioning

confidence: 99%

Indole derivatives inhibited the formation of bacterial biofilm and modulated Ca2+ efflux in diatom

Yang

Yin

Sun

et al. 2014

Marine Pollution Bulletin

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“…S3) and may have evolved to address a eukaryotic cellular need. Specifically, Ca, with its high coordination number and affinity for hard ligands, is ideally suited for signaling between organelles or cells (3,38). Supporting this hypothesis, the most abundant Ca-binding domains in Eukarya are predominantly involved in signaling within or between cells (Table S4).…”

Section: Emergence Of the Akaryotes Metal Homeostasis And Biogeochementioning

confidence: 95%

History of biological metal utilization inferred through phylogenomic analysis of protein structures

Dupont

Butcher

Valas³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

286

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The fundamental chemistry of trace elements dictates the molecular speciation and reactivity both within cells and the environment at large. Using protein structure and comparative genomics, we elucidate several major influences this chemistry has had upon biology. All of life exhibits the same proteome size-dependent scaling for the number of metal-binding proteins within a proteome. This fundamental evolutionary constant shows that the selection of one element occurs at the exclusion of another, with the eschewal of Fe for Zn and Ca being a defining feature of eukaryotic proteomes. Early life lacked both the structures required to control intracellular metal concentrations and the metal-binding proteins that catalyze electron transport and redox transformations. The development of protein structures for metal homeostasis coincided with the emergence of metal-specific structures, which predominantly bound metals abundant in the Archean ocean. Potentially, this promoted the diversification of emerging lineages of Archaea and Bacteria through the establishment of biogeochemical cycles. In contrast, structures binding Cu and Zn evolved much later, providing further evidence that environmental availability influenced the selection of the elements. The late evolving Zn-binding proteins are fundamental to eukaryotic cellular biology, and Zn bioavailability may have been a limiting factor in eukaryotic evolution. The results presented here provide an evolutionary timeline based on genomic characteristics, and key hypotheses can be tested by alternative geochemical methods.Archean-Proterozoic | biogeochemistry | bioinorganic chemistry | evolution | metal homeostasis M etalloproteins contain one or more ions of an inorganic element in their 3D structure and are said to comprise 30% of all proteins (1). Many biological pathways contain at least one metalloenzyme (2) and consequently require Mg, K, Ca, Fe, Mn, and Zn to sustain life (3). Other elements, like Cu, Mo, Ni, Se, and Co, are required by many-though not all-organismal lineages, and the utilization of trace elements varies greatly between species (3). The different elements have a range of affinities for most coordinating environments in the order Mg +2 /Ca +2 < Mn +2 < Fe +2 < Co +2 < Ni +2 < Cu +2 ∼Zn +2 , an equilibrium series known as the Irving-Williams Series (4). This array of outer-sphere chemistry provides significant catalytic diversity, yet has consequences for both biological and environmental chemistry. Within the cell, metals compete for protein binding sites; hence, extensive protein networks involving transporters and metalsensing regulatory proteins are required to maintain the proper subcellular concentrations of each element, and in some cases directly shuttle each metal to its requisite metalloprotein in the proper cellular compartment (1, 5, 6). It has been hypothesized that the establishment of a metal homeostasis system is required for distinct phylotypes of cells to develop (7).Environmentally, for similar fundamental chemical reasons, the...

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A Stress Surveillance System Based on Calcium and Nitric Oxide in Marine Diatoms

Cited by 267 publications

References 50 publications

Whole-cell response of the pennate diatom Phaeodactylum tricornutum to iron starvation

Whole-cell response of the pennate diatom Phaeodactylum tricornutum to iron starvation

Indole derivatives inhibited the formation of bacterial biofilm and modulated Ca2+ efflux in diatom

History of biological metal utilization inferred through phylogenomic analysis of protein structures

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