A Strip Biosensor with Guinea Green B and Fuchsin Basic Color Indicators on a Glass Nanofiber Carrier for the Cholinesterase Detection of Nerve Agents

Abstract

This paper deals with the innovation of the Czech colorimetric biosensor Detehit designed for the simple, fast, and sensitive detection of nerve agents. The innovation is based on the use of an indicator consisting of a mixture of two triphenylmethane dyes, Guinea green B and a basic fuchsin, on a glass nanofiber filter paper carrier. The advantage of this solution is the blue–red color transition, which is much more visible than the white–yellow transition of other Detehit biosensors. The newly designed biosensor allows the users to visually detect (with the naked eye) the presence of the most significant paralytic substances (sarin, soman, cyclosarin, tabun, VX) in water at concentrations of at least 0.001 μg/mL. This biosensor design also enables one to detect these substances in air or on contaminated surfaces.

Recent advances in fluorescent and colorimetric chemosensors for the detection of chemical warfare agents: a legacy of the 21st century

“…29) in water. 172 Both dyes are sensitive to thiolate attack via Michaeltype addition, which changes the optical properties in the form of a blue-red transition (i.e., from l max 620 nm and 540 nm). The authors have also examined the stability of the sensing system by impregnating the reagents/substrates and dye into the glass nanofiber filter paper for NA detection to successfully demonstrate field applications.…”

“…29) in water. 172 Both dyes are sensitive to thiolate attack via Michael-type addition, which changes the optical properties in the form of a blue–red transition ( i.e. , from λ max 620 nm and 540 nm).…”

Simple Chemical and Cholinesterase Methods for the Detection of Nerve Agents Using Optical Evaluation

“…The most widely used is probably Guinea Green B, designed for the biosensor for NAs detection [53] and earlier for the Soviet GSP-12 (Figure 4) automatic tape analyser, which replaced the obsolete GSP-11. A mixture of Guinea Green B with another triphenylmethane dye, Fuchsin Basic, which changes the blue colour to red-violet by reduction, was proposed for the preparation of the detection tube and the surface biosensor for NAs detection [54]. In all these means with triphenylmethane dyes, BChE was preferentially used.…”

Development of chromogenic detection for biomolecular analysis

“…[ 95 ] Some researches constructed more efficient, convenient, and simple platforms based on chemical indicators with final color signal readout which was more obvious with a low background signal. [ 96 ] As a chemical dye indicator, the ABTS can be used in concentration nitric oxide detection in cells. Based on the color change of ABTS in the gas flow.…”