1996
DOI: 10.1007/bf01718401
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A structural DNA binding protein of African swine fever virus with similarity to bacterial histone-like proteins

Abstract: Here we describe an African swine fever virus (ASFV) protein encoded by the open reading frame 5-AR that shares structural and functional similarities with the family of bacterial histone-like proteins which include histone-like DNA binding proteins, integration host factor, and Bacillus phage SPO1 transcription factor, TF1. The ASFV 5-AR gene was cloned by PCR and expressed in E. coli. Monospecific antiserum prepared to the 5-AR bacterial expression product specifically immunoprecipitated a protein of approxi… Show more

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Cited by 41 publications
(37 citation statements)
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“…The pEL-GFP-TPR construct has been previously described (45). pET-21a-5AR was provided by Manuel Borca (Plum Island Animal Disease Center, New York, N.Y.) (8) and was used as a template to amplify the 5AR open reading frame by using the primers 5Ј-GGGGCGGCCG CTCGACAAAAAAAAAG-3Ј and 5Ј-TTTGAATTCTTAATTTAACATATC-3Ј. The resulting PCR products were cloned into the NotI-EcoRI sites of the expression vector pEL-GFP (20) to generate 5AR tagged at the N terminus with green fluorescent protein (GFP-5AR).…”
Section: Methodsmentioning
confidence: 99%
“…The pEL-GFP-TPR construct has been previously described (45). pET-21a-5AR was provided by Manuel Borca (Plum Island Animal Disease Center, New York, N.Y.) (8) and was used as a template to amplify the 5AR open reading frame by using the primers 5Ј-GGGGCGGCCG CTCGACAAAAAAAAAG-3Ј and 5Ј-TTTGAATTCTTAATTTAACATATC-3Ј. The resulting PCR products were cloned into the NotI-EcoRI sites of the expression vector pEL-GFP (20) to generate 5AR tagged at the N terminus with green fluorescent protein (GFP-5AR).…”
Section: Methodsmentioning
confidence: 99%
“…Two fixatives, periodate-lysine-paraformaldehyde (PLP; Nakane) and 4% freshly prepared paraformaldehyde, and three embedding media (Lowicryl K4M, London Resin White, and Unicryl) were used for IEM of sectioned material. Cells were infected with Malawi Lil-20/1 and processed with the above fixatives and embedding media as previously described (5). The number of gold grains associated with 50 virions was determined.…”
Section: Methodsmentioning
confidence: 99%
“…IHF binding sites are also found in a large number of promoters and a gene expression pro®le in Escherichia coli identi®ed 150 genes differentially expressed in IHF + and IHF ± -strains (10). In eukaryotes, IHF is present in chloroplasts, African swine virus and shares sequence and structural homology with the ubiquitous TATA box binding protein (9,11,12).…”
Section: Introductionmentioning
confidence: 99%