Online examinations are an integral component of many online learning environments, which face many security challenges. Collusion is seen as a major security threat to such examinations, when a student invites a third party to impersonate or abet in a test. This work aims to strengthen the authentication of students via the use of dynamic profile questions. The study reported in this paper involved 31 online participants from five countries over a five-week period. The results of usability and security analysis are reported. The dynamic profile questions were more usable than both the text-based and image-based questions (p < 0.01). An impersonation abuse scenario was simulated using email and mobile phone. The impersonation attack via email was not successful, however, students were able to share answers to dynamic profile questions with a third party impersonator in real time, which resulted in 93% correct answers. The sharing of information via phone took place in real time during Abrar Ullah ( ) Llandaff Campus, Cardiff Metropolitan University, Cardiff, CF5 2YB, UK e-mail: aaaullah@cardiffmet.ac.uk Hannan Xiao · Trevor Barker College Lane Campus, University of Hertfordshire, Hatfield, AL10 9AB, UK e-mail: h.xiao@herts.ac.ukTrevor Barker e-mail: t.1.barker@herts.ac.uk an online test and the response time of an impersonator was significantly different (p < 0.01) than a student. The study also revealed that a response time factor may be implemented to identify and report impersonation attacks.