A study of 99mtechnetium-labelled beclomethasone dipropionate dilauroylphosphatidylcholine liposome aerosol in normal volunteers

Vidgrén, M.; Waldrep, J. Clifford; Arppe, J.; Black, Melanie; Rodarte, J.A.; Cole, William R.; Knight, Vernon

doi:10.1016/0378-5173(94)00265-7

Cited by 60 publications

(39 citation statements)

References 14 publications

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“…PEI-DNA complexes were aerosolized using an Aerotech II nebulizer (AT-II) (CIS-US, Inc., Bedford, MA) at 10 liters/min flow rate using air or air containing 5% CO 2 . AT-II is a high-output, efficient nebulizer demonstrated to produce aerosols in the optimal range of 1-2 µm mass median aerodynamic diameter (MMAD) for peripheral lung delivery (17,18). The PEI-DNA aerosol particle size was calculated to be 1.6 µm MMAD with a geometric standard deviation (GSD) of 2.9 using an Andersen cascade impactor (Andersen Instruments, Atlanta, GA) by a method previously described (18).…”

Section: Methodsmentioning

confidence: 99%

“…However, this is a rather invasive technique compared to aerosol and generally results in less uniform deposition to the peripheral regions of the lung. Aerosol delivery helps to distribute the particles noninvasively and uniformly throughout the lungs (17). We are currently examining PEI-mediated gene expression in lung histologically following aerosol delivery of PEI-DNA complexes.…”

Section: Figmentioning

confidence: 98%

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Enhanced Gene Expression in Mouse Lung after PEI–DNA Aerosol Delivery

et al. 2000

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Aerosol gene delivery to the pulmonary system has vast potential for many diseases, including cystic fibrosis and lung cancer. We recently reported that polyethyleneimine (PEI), a cationic polymer, holds promise as a gene delivery vector for transfection in lung by aerosol. To further optimize the gene expression in the lung by aerosol, we utilized 5% CO(2) in air for the nebulization of PEI-DNA complexes. Five percent CO(2)-in-air gave a threefold higher gene expression compared to normal air using the chloramphenicol acetyl transferase (CAT) reporter gene delivered by Aerotech II nebulizer. The delivery of DNA by PEI was dose dependent with the highest expression obtained when 2 mg of DNA in 10 ml was nebulized at a PEI nitrogen:DNA phosphate (N:P) ratio of 10:1. The optimal N:P ratio for lung transfection was found to be between 10:1 and 20:1 using the CAT and luciferase reporter genes. The time-course studies showed the highest expression at 24 h after aerosol delivery and 40-50% of peak level was detectable even after a week. Tissue distribution indicates the expression to be specific to the lung with no detectable expression in any other tissue examined. Histological and biochemical analysis of lungs revealed no evidence of acute inflammation.

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Section: Methodsmentioning

confidence: 99%

Section: Figmentioning

confidence: 98%

Enhanced Gene Expression in Mouse Lung after PEI–DNA Aerosol Delivery

et al. 2000

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“…Human studies of liposome aerosols have demonstrated similar retention within the normal lung, but, depending on the presence of infl ammation (Vidgren et al 1994;Saari et al 1998). Factors affecting the clearance and/or elimination of exogenous materials from normal and diseased lung tissues are not yet completely understood.…”

Section: Liposomesmentioning

confidence: 99%

“…Using gamma scintigraphy, nebulizer systems were evaluated and selected for optimal aerosol targeting of drug-liposomes to the peripheral lung regions (Vidgren et al 1994). However, recent studies of CsA-liposomes in other experimental systems have reported that CsA displays a unique property of rapid bilayer membrane exchange Fahr et al 1995;Ouyang et al 1995).…”

Section: Liposomesmentioning

confidence: 99%

Nanocarriers as pulmonary drug delivery systems to treat and to diagnose respiratory and non respiratory diseases

Vandamme¹

2008

IJN

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Abstract:The purpose of this review is to discuss the impact of nanocarriers administered by pulmonary route to treat and to diagnose respiratory and non respiratory diseases. Indeed, during the past 10 years, the removal of chlorofl uorocarbon propellants from industrial and household products intended for the pulmonary route has lead to the developments of new alternative products. Amongst these ones, on one hand, a lot of attention has been focused to improve the bioavailability of marketed drugs intended for respiratory diseases and to develop new concepts for pulmonary administration of drugs and, on the other hand, to use the pulmonary route to administer drugs for systemic diseases. This has led to some marketed products through the last decade. Although the introduction of nanotechnology permitted to step over numerous problems and to improve the bioavailability of drugs, there are, however, unresolved delivery problems to be still addressed. These scientifi c and industrial innovations and challenges are discussed along this review together with an analysis of the current situation concerning the industrial developments.

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“…Beclomethasone dipropionate (BD; C 38 H 28 ClO 7 ; MW: 521.0 Da) (Figure 1), a synthetic chlorinated glucocorticoid diester, is a well-established antiinflammatory agent used in the treatment of asthma (Vidgren et al, 1995). BD is characterized by a white or almost white crystalline powder, presenting pKa of 13.6 and melting range between 117-120 °C (Wilcox, Avery, 1973;Brogden et al, 1984;Barnes, 2010;ECP, 2010).…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating RP-HPLC method for determination of beclomethasone dipropionate in nanocapsule suspensions

Chassot

Ferreira

Gomes

et al. 2015

Braz. J. Pharm. Sci.

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A simple stability-indicating RP-HPLC/UV method was validated for determination of beclomethasone dipropionate (BD) in nanocapsule suspensions. Chromatographic conditions consisted of a RP C 18 column (250 mm x 4.60 mm, 5 µm, 110 Å), using methanol and water (85:15 v/v) as mobile phase at 1.0 mL/ min with UV detection at 254 nm. The calibration curve was found to be linear in the concentration range of 5.0-25.0 µg/mL with a correlation coefficient > 0.999. Precision was demonstrated by a relative standard deviation lower than 2.0%. Accuracy was assessed by the recovery test of BD from nanocapsules (98.03% to 100.35%). Specificity showed no interference from the components of nanocapsules or from the degradation products derived from acid, basic and photolytic conditions. In conclusion, the method is suitable to be applied to assay BD in bulk drug and in nanocapsules, and it can be employed to study stability and degradation kinetics.Uniterms: High performance liquid chromatography/reverse phase/quantitative analysis. Beclomethasone dipropionate/determination. Nanocapsule/suspensions. Nanoparticles.Um método simples de CLAE-FR/UV indicativo de estabilidade foi validado para a determinação do dipropionato de beclometasona (BD) em suspensões de nanocápsulas. As condições cromatográficas foram: coluna C18 fase reversa (250 mm x 4,60 mm, 5 µm, 110 Å), usando como fase móvel metanol e água (85:15 v/v) a 1,0 mL/min, com detecção UV a 254 nm. A curva de calibração foi linear no intervalo de 5,0-25,0 µg/mL com coeficiente de correlação >0,999. A precisão foi demonstrada por um desvio padrão relativo menor que 2,0%. A exatidão foi avaliada pelo teste de recuperação do BD a partir das nanocápsulas (98,03% a 100,35%). O teste de especificidade não mostrou interferência dos componentes das nanocápsulas e nem dos produtos de degradação derivados de condições ácidas, básicas e fotolíticas. Em conclusão, o método é adequado para ser aplicado na avaliação do BD puro e em nanocápsulas e pode ser empregado para o estudo de estabilidade e degradação cinética.Unitermos: Cromatografia líquida de alta eficiência/fase reversa/análise quantitativa. Dipropionato de beclometasona/determinação. Nanocápsulas/suspensões. Nanopartículas.

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A study of 99mtechnetium-labelled beclomethasone dipropionate dilauroylphosphatidylcholine liposome aerosol in normal volunteers

Cited by 60 publications

References 14 publications

Enhanced Gene Expression in Mouse Lung after PEI–DNA Aerosol Delivery

Enhanced Gene Expression in Mouse Lung after PEI–DNA Aerosol Delivery

Nanocarriers as pulmonary drug delivery systems to treat and to diagnose respiratory and non respiratory diseases

Stability-indicating RP-HPLC method for determination of beclomethasone dipropionate in nanocapsule suspensions

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