A Study of Bone-Like Apatite Formation on Calcium Phosphate Ceramics in Different Simulated Body Fluids (SBF)

Abstract

Five kinds of simulated body fluids were prepared according to the Ca 2+ ions concentration in body fluids of human, dog, pig, rabbit and monkey so that the different biological environments in different animals were simulated. Results showed that Ca 2+ can induce bone-like apatite formation in a dose-dependent manner; there is a threshold of Ca 2+ local concentration for the formation of bone-like apatite. The threshold of Ca 2+ for bone-like apatite formation in static stimulated body fluid (SBF) is different from dynamic SBF. The threshold of static SBF is lower than that of dynamic SBF. The threshold of Ca 2+ local concentration is 0.2459g/L in static SBF and 0.3392g/L in dynamic SBF.

The study on the degradation and mineralization mechanism of ion‐doped calcium polyphosphate in vitro

“…The MCPP sample obviously contained a greater mineralized layer than the others. The 21 represented hydroxyl. Additionally, the XRD analysis on the doped CPP surface with mineral deposit for 28-day degradation in Figure 6 also indicated the characteristic peaks of HA in (211), (112), (300), and (202).…”

“…The crystal lattice parameters in Table II were calculated by curve fitting and indicated Na 1 , Mg 21 , Zn 21 substituting in the site of Ca 21 and the cell volumes of Na-, Mg-, and Zn-doped CPP shrank while those of K-and Sr-doped CPP expanded. Figure 2 shows the XPS binding energies on the surface of KCPP, NCPP, MCPP, ZCPP, and SCPP samples before degradation.…”

“…The degradation process of bone substitute in vivo significantly affects the biomineralization process on implants, because the released ions provide abundant ionic resources for surface nucleation. de Bruijin et al 4 point out that the formation of biomineralized apatite layer on the surface of biomaterials is the prerequisite of osteoinduction and osteoconduction, and the formation of apatite layer is the consequence of the mineralized process according to the concentration increase of Ca 21 and PO 4 32 . Thus, a controllable degradation rate of bone repair material in vitro study is meaningful because it can be adjusted to match with the mineralization rate and consequently affect osteoinduction and osteoconduction process in vivo.…”

An histological study of the influence of osteoinductive calcium phosphate ceramics on tendon healing pattern in a bone tunnel with suspensory fixation

“…Ishikawa et al [5] injected a gelformed composite of collagen and powdered hydroxyapatite (C-HAp) into the bone tunnel to enhance healing between tendon and bone in an extra-articular animal model. The study showed that new bone formation and Sharpey's fibres emerging in the C-HAp group was much more than that in the control group before 8 weeks, but no Sharpey's fibres were observed [3]. Tien et al performed suspensory fixation ACL reconstruction in a rabbit model with special solubility calcium phosphate cement (CPC) to fill the gap between tendon and bone tunnel.…”

“…By contrast, much new bone formed in the samples not inhibited by McAb-BMP [24]. Concentration of the intrinsic biological signal (such as BMP) to the surface of porous calcium phosphate ceramics induced the mesenchymal cells to differentiate into preosteogenic cells [3]. The threedimensional porous structure and the formation of bonelike apatite interface provided a suitable environment for osteogenesis [3].…”