A study of elastic tissue and actinic radiation in “aging,” temporal arteritis, polymyalgia rheumatica, and atherosclerosis

O’Brien, John P.; Regan, W. M.

doi:10.1016/0190-9622(91)70118-l

Cited by 41 publications

(27 citation statements)

References 77 publications

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“…The extent of progressive accumulation of elastic fibers containing in particular elastin and fibrillin 1 is dependent on the degree of sun exposure (32). In our volunteers, only the mRNA of fibrillin 1 was significantly increased after repeated exposures, whereas the level of elastin mRNA did not change upon one or repeated SSR exposure, which further supports fibrillin 1 as a marker of photoreaction.…”

Section: Discussionsupporting

confidence: 65%

Changes in Matrix Gene and Protein Expressions After Single or Repeated Exposure to One Minimal Erythemal Dose of Solar-simulated Radiation in Human Skin In Vivo

Seité¹,

Colige²,

Deroanne³

et al. 2004

Photochem Photobiol

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Damage to the skin extracellular matrix (ECM) is the hallmark of long-term exposure to solar UV radiation. The aim of our study was to investigate the changes induced in unexposed human skin in vivo after single or repeated (five times a week for 6 weeks) exposure to 1 minimal erythemal dose (MED) of UV solar-simulated radiation. Morphological and biochemical analyses were used to evaluate the structural ECM components and the balance between the degrading enzymes and their physiologic inhibitors. A three-fold increase in matrix metalloproteina.se 2 messenger RNA (mRNA) (P < 0.02, unexposed versus exposed) was observed after both single and repeated exposures. Fibrillin 1 mRNA level was increased by chronic exposure (P < 0.02) and unaltered by a single MED. On the contrary, a single MED significantly enhanced mRNA levels of interleukin-1α (IL-1α), IL-1β (P < 0.02) and plasminogen activator inhibitor-1 (P < 0.05). Immunohisto-chemistry demonstrated a significant decrease in Type-I procollagen localized just below the dermal-epidermal junction in both types of exposed sites. At the same location, the immunodetected tenascin was significantly enhanced, whereas a slight increase in Type-IΠ procollagen deposits was also observed in chronically exposed areas. Although we were unable to observe any change in elastic fibers in chronically exposed buttock skin, a significant increase in lysozyme and alpha-1 antitrypsin deposits on these fibers was observed. These results demonstrate the existence of a differential regulation, after chronic exposure compared with an acute one, of some ECM components and inflammatory mediators.

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Section: Discussionsupporting

confidence: 65%

Changes in Matrix Gene and Protein Expressions After Single or Repeated Exposure to One Minimal Erythemal Dose of Solar-simulated Radiation in Human Skin In Vivo

Seité¹,

Colige²,

Deroanne³

et al. 2004

Photochem Photobiol

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“…Increased activity of a serine elastase is also present in aorta organ culture in association with the development of neointimal formation (30). In the clinical setting, increased elastolytic activity has also been described in atherosclerosis (11) and in a vasculopathy induced by actinic radiation (31 ).…”

Section: Discussionmentioning

confidence: 96%

The endogenous vascular elastase that governs development and progression of monocrotaline-induced pulmonary hypertension in rats is a novel enzyme related to the serine proteinase adipsin.

Zhu¹,

Wigle

Hinek³

et al. 1994

J. Clin. Invest.

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We showed previously a cause and effect relationship between increased activity of an endogenous vascular elastase (EVE) and experimentally induced pulmonary hypertension in rats. We now report the isolation and characterization of EVE. Degenerate oligonucleotides synthesized to homologous sequences in serine elastases were used in a PCR with rat pulmonary artery (PA) cDNA. The PCR product hybridized to a 1.2-kb mRNA and the intensity of hybridization was threefold increased in RNA from rat hypertensive PA at a timepoint when EVE activity was increased. The PCR product was used to screen a cDNA library and sequences obtained encoded rat adipsin. We then used immunoaffinity to purify EVE. An antibody to the elastin-binding protein was used to remove this competitor of elastase from the PA extract and the elastolytic activity increased 100-fold. The enzyme was purified using an antibody that recognizes NH2-terminal sequences of serine proteinases and the eluate was further purified using an antibody raised against recombinant adipsin. A single band at 20 kD immunoreactive with the adipsin antibody was resolved as an active enzyme on an elastin substrate gel. Immunogold labeling with an antibody to an adipsin peptide sequence localized EVE to PA smooth muscle cells. This is the first isolation of EVE; it appears to be a novel enzyme related to the serine proteinase adipsin originally found in adipose tissue. (J.

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“…Temporal arterial biopsies were taken from subjects suspected of having temporal arteritis because of unexplained inflammation. Cases of temporal arteritis associated with polymyalgia rheumatica were excluded from this study [9]. Temporal arterial biopsies were taken at the same time as the forehead skin (skin exposed to sunlight) and at the same site, thus allowing us to know the orientation of the temporal artery that we divided arbitrarily into two parts: the 'superficial part' of the temporal artery is the one exposed to sunlight or 'weather' side, and the 'deep part' is the side opposite to temporal bone.…”

Section: Methodsmentioning

confidence: 99%

Measurement of the Amounts of Elastic Fibers in the Skin and Temporal Arteries of Healthy Aged Individuals by Automated Image Analysis

Gogly¹,

Godeau²,

Septier

et al. 1998

Gerontology

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Background: A quantitative study of dermal and arterial elastic fibers as a function of age was carried out by computerized image analysis. Objective: We investigated whether any parallelism can be established between the morphometric parameters of elastic fibers from the skin and the temporal artery in elderly subjects. Methods: we quantitated the skin elastic fibers of the reticular dermis and the elastic fibers of the temporal artey using a specific staining procedure followed by automated image analysis in 16 subjects of age range 63–87 years. Results: There was a good correlation between the area fraction occupied by the elastic fibers in the unexposed skin (inner part of the upper arm) and aging (r = 0.669, p < 0.01). The area fraction occupied by elastic fibers in unexposed skin was correlated with the area fraction occupied by elastic fibers in the deep part of the temporal artery (r = 0.498, p < 0.05). Actinic elastosis affected both tissues, but there was no correlation between the amount of elastotic material in the exposed skin and the area fraction of elastic fibers in the superficial part of the temporal artery. Conclusion: We provided evidence that in sun-protected tissues the area fraction occupied by elastic fibers in dermis and deep part of the temporal artery showed a significant correlation. We proposed that skin biopsies were a valuable diagnostic tool for predicting arterial wall abnormalities of elastic fibers.

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A study of elastic tissue and actinic radiation in “aging,” temporal arteritis, polymyalgia rheumatica, and atherosclerosis

Cited by 41 publications

References 77 publications

Changes in Matrix Gene and Protein Expressions After Single or Repeated Exposure to One Minimal Erythemal Dose of Solar-simulated Radiation in Human Skin In Vivo

Changes in Matrix Gene and Protein Expressions After Single or Repeated Exposure to One Minimal Erythemal Dose of Solar-simulated Radiation in Human Skin In Vivo

The endogenous vascular elastase that governs development and progression of monocrotaline-induced pulmonary hypertension in rats is a novel enzyme related to the serine proteinase adipsin.

Measurement of the Amounts of Elastic Fibers in the Skin and Temporal Arteries of Healthy Aged Individuals by Automated Image Analysis

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