A study of epithelial-mesenchymal transition immunohistochemical markers in primary oral squamous cell carcinoma

Nambiyar, Kaniyappan; Ahuja, Arvind; Bhardwaj, Minakshi

doi:10.1016/j.oooo.2021.05.016

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…[ 10 ] evaluated the E-cadherin and N-cadherin immunoexpression in OSCC and observed even lower E-cadherin expression in the invasion fronts and highly invasive tumours. According to Nambiyar et al ,[ 24 ] E-cadherin expression was significantly reduced in cases with lymph node metastasis; however, this study did not analyse expression in the invasive front separately, because the tumour depth orientation was not feasible due to the small biopsies obtained. In our study, it was possible to detect the reduction in E-cadherin expression in the invasion front in some cases of moderately differentiated OSCC, but the association of E-cadherin expression with the clinical–pathological profile was not established.…”

Section: Discussionmentioning

confidence: 94%

Differential expression of Cadherins switch and Caveolin-2 during stages of oral carcinogenesis

Nascimento,

Machado,

Silva

et al. 2023

Journal of Oral and Maxillofacial Pathology

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Background: Oral squamous cell carcinoma (OSCC) accounts for 90% of oral malignancies, which may be preceded by oral potentially malignant disorders (OPMDs). Cancer progression involves the downregulation of epithelial markers (E-cadherin) and the upregulation of mesenchymal markers (N-cadherin), which together characterise the epithelial–mesenchymal transition (EMT). Furthermore, caveolin can act on cell adhesion and migration events that regulate the expression of the E-cadherin/α-β-catenin complex, thus favouring aggressive biological behaviour. This study aimed to analyse the immunoexpression of E-cadherin, N-cadherin and caveolin-2 at different stages of oral carcinogenesis to identify reliable biomarkers to predict malignant potential. Methods: Expressions of E-cadherin and N-cadherin in 14 normal oral mucosae (NOM), 14 OPMD and 33 OSCC specimens were evaluated using immunohistochemistry. Clinicopathological parameters were also assessed. Results: E-cadherin immunoexpression was significantly reduced during the progression of oral carcinogenesis (P = 0.0018). N-cadherin immunoexpression did not show any statistical differences between these groups. However, a representative number of N-cadherin-positive OSCC cases did not express E-cadherin. The expression of caveolin-2 increased significantly with the progression of the disease, from NOM to OSCC (P value: 0.0028). Conclusion: These findings indicate that cadherin switch and caveolin-2 immunoexpression may be regulatory events in oral carcinogenesis.

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Section: Discussionmentioning

confidence: 94%

Differential expression of Cadherins switch and Caveolin-2 during stages of oral carcinogenesis

Nascimento,

Machado,

Silva

et al. 2023

Journal of Oral and Maxillofacial Pathology

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“…E-cadherin is one of the markers of epithelial-mesenchymal transition (EMT) which is related to tumor metastasis [ 23 ] and Ki67 indicated cell proliferation. To explore the role of IRF6 in CRC, the expression of IRF6, E-cadherin and Ki67 was measured in CRC tissue.…”

Section: Resultsmentioning

confidence: 99%

The interferon regulatory factor 6 promotes cisplatin sensitivity in colorectal cancer

Lin

et al. 2022

Bioengineered

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Colorectal cancer (CRC) is one of the most common malignancies and causes of cancer-related mortality worldwide. Cell proliferation and tumor metastasis as well as chemoresistance are correlated with poor survival of CRC. The interferon regulatory factor 6 (IRF6) is functioned as a tumor suppressor gene in several cancers and is associated with risk of CRC. We explored the role of IRF6 in CRC in the present study. The protein expressions of IRF6 in human CRC tissues, normal para-carcinoma tissue and liver metastases from CRC were measured. Cell proliferation, chemotherapeutic sensitivity, cell apoptosis, migration and invasion including the related markers along with IRF6 expression were explored. Our results indicated that IRF6 expression in CRC and liver metastasis were lower than normal tissues, which were correlated positively with E-cadherin and negatively with Ki67 expression in CRC tissue. IRF6 promoted CRC cell sensitivity to cisplatin to suppress cell proliferation, migration and invasion as well as aggravate cell apoptosis. Our study suggested that IRF6 may enhance chemotherapeutic sensitivity of cisplatin mediated by affecting cell proliferation, migration and invasion along with apoptosis through regulating E-cadherin and Ki67, while the identified molecular mechanisms remain to be further explored.

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“…The study included 50 cases in total [18]. A recent study conducted by Nambiyar et al (2021) in India also found that 35 out 42% of patients showed a lack of E-cadherin expression, particularly around the invasive edge of the tumor [22]. An Indian study [23] observed that 80% of cases of OSCC exhibited an upregulation of N-cadherin expression.…”

Section: Discussionmentioning

confidence: 99%

Expression of Epithelial-Mesenchymal Transition Markers in Oral Cavity Squamous Cell Cancer: An Observational Study

Solanki,

Malhotra,

Singh

et al. 2024

Preprint

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Background Epithelial-Mesenchymal transition (EMT) has evolved as a possible pathway in pathophysiology of tumor formation and progression; however, contribution of this phenomenon in human cancers especially oral malignancy lack sufficient evidence. This study aimed to evaluate the expression of EMT markers E-cadherin and N-cadherin and their correlation with clinicopathological factors in Oral Cavity Squamous Cell Cancer (OSCC). Methods This observational study was conducted on hundred histologically proven cases of OSCC based on inclusion and exclusion criteria. The expression level of Epithelial-Mesenchymal Transition markers (E-cadherin, N-cadherin) on these cases was performed and evaluated using Immunohistochemistry (IHC) staining. Expression of E-cadherin, N-cadherin was analysed and correlated with clinical and histopathological factors using appropriate statistical methods. Results Epithelial markers E-cadherin and N-cadherin were expressed in 18 (18%) and 58 (58%). Reduced expression of E-cadherin was observed in 82 (82%) patients. Significant association of N-Cadherin with clinical T-stage (p = 0.001) and clinical N-stage (p = 0.041) was observed. Pathological factors such as tumour size (p = 0.016), DOI (p = 0.001), pathological T-stage (p = 0.005) and stage (p = 0.017) showed statistically significant association with N-cadherin expression. Conclusion This study emphasizes the importance of EMT in the pathogenesis of OSCC. The use of biomarkers like E-Cadherin, N-cadherin might serve as valuable tool for developing targeted therapy in near future limiting invasion and metastasis in OSCC.

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A study of epithelial-mesenchymal transition immunohistochemical markers in primary oral squamous cell carcinoma

Cited by 7 publications

References 13 publications

Differential expression of Cadherins switch and Caveolin-2 during stages of oral carcinogenesis

Differential expression of Cadherins switch and Caveolin-2 during stages of oral carcinogenesis

The interferon regulatory factor 6 promotes cisplatin sensitivity in colorectal cancer

Expression of Epithelial-Mesenchymal Transition Markers in Oral Cavity Squamous Cell Cancer: An Observational Study

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