A Study of Estrogen Fluorescence Using Ittrich's Procedure

Scholler, R; Leymarie, P.; M, Heron; Jayle, M F

doi:10.1530/acta.0.052s007

Cited by 10 publications

(9 citation statements)

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“…The excitation and emission spectra of the fluorescence in these three solvent systems have been carefully reassessed in a recent study by Scholler et al (1966). Their findings are in complete agreement with our own and are as follows: (1) The wavelengths for maximum excitation/emission using chloroform or tetrachloroethane were 535-536 m/i/549-550 rap for oestriol, oestrone and oestradiol, and 538-539 rap/ 550-551 rap for their 3-methyl ethers; in tetrabromoethane, the corresponding wave¬ lengths were 541-542 m/(/560-561 rap for the oestrogens and 544-545 rap/563 rap for their methyl ethers.…”

Section: Ittrich Fluorescence Procedures Extraction and Measurement Ofmentioning

confidence: 99%

“…Ittrich (1958) and later workers (Stoa & Thorsen, 1962;Scholler et al 1966) also employed a one-stage reaction in which the sulphuric acid concentration for oestriol and oestrone was 65-68% and the heating time was 40 min. at 100°.…”

Section: Calculation Of ' K ' For Impuritiesmentioning

confidence: 99%

“…Ittrich (1958) showed that the red pigment formed by oestrogens in the Kober reaction was extractable with a solution of 2 %^j-nitrophenol in chloroform and that the complex could then be measured by colorimetry or fluorimetry. This reaction was also extensively studied (Ittrich, 1960;Stoa & Thorsen, 1962; Scholler, Leymarie, Heron & Jayle, 1966). The Kober reaction combined with the Ittrich extraction provides a highly specific system for measuring many of the natural oestrogens.…”

Section: Introductionmentioning

confidence: 99%

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Further Observations on the Kober Colour and Ittrich Fluorescence Reactions in the Measurement of Oestriol, Oestrone and Oestradiol

Brown¹,

Macnaughtan²,

Smith³

et al. 1968

Journal of Endocrinology

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Higher temperatures allow lower sulphuric acid concentrations and shorter heating times to be used in the Kober colour reaction for oestrogens. A one-stage reaction which is completed in 5 min. at 120\s=deg\is described for oestrone and oestradiol, and a two-stage reaction which requires two periods of heating for 5 min. at 120\s=deg\is described for oestriol. The conditions were applied to the Ittrich fluorescence procedure. A spectrophotofluorimetric correction was developed in which fluorescence was measured at wavelengths for excitation and emitted light near the optima for the oestrogens and at another combination at which the oestrogens produced virtually no fluorescence whereas that of impurities was not diminished. Extraction, centrifugation and fluorimetry were performed in specially designed cells. The sensitivity is 0\ m=. \ 05\ p=n-\ 0\ m=. \ 1 ng./sample with a linear response up to 300 ng. and a precision better than 4% in the range 1\m=.\0\p=n-\100 ng.

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Section: Ittrich Fluorescence Procedures Extraction and Measurement Ofmentioning

confidence: 99%

Section: Calculation Of ' K ' For Impuritiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Further Observations on the Kober Colour and Ittrich Fluorescence Reactions in the Measurement of Oestriol, Oestrone and Oestradiol

Brown¹,

Macnaughtan²,

Smith³

et al. 1968

Journal of Endocrinology

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“…Recently, several rapid methods have been published for measuring one or a mixture of oestrogens in urine in which the highly specific Ittrich fluorescence pro¬ cedure has been applied to relatively impure phenolic extracts (Stoa & Thorsen, 1962 ;Eechaute & Demeester, 1965;Scholler, Leymarie, Heron & Jayle, 1966;Strickler, Holt, Acevedo, Saier & Grauer, 1967). The accuracy and precision of these methods have been reported and specificity has been indicated by the similarity between the fluorescence spectra produced by urine extracts and oestrogen standards.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Method for Estimating Oestrogens in Urine Using a Semi-Automatic Extractor

Brown¹,

MacLeod²,

Macnaughtan³

et al. 1968

Journal of Endocrinology

417

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A semi-automatic extractor was developed which processes 12 urines at a time to a stage where 'total' oestrogens can be measured by colorimetry using the Kober reaction (late pregnancy urines) or by fluorimetry using the Kober\p=m-\Ittrich procedure (non-pregnancy and early pregnancy urines). One worker can complete 12 analyses in 3\ m=1/ 2\ hr. or 24\p=n-\36 in a working day. The results obtained at oestrogen levels above 1 mg./24-hr. urine were the same as those obtained by a method specific for oestriol. The results obtained from non-pregnancy urines were compared with the sums of oestriol, oestrone and oestradiol obtained by the method of Brown (1955). The mean ratio (\ m=+-\ s.d.) of the two values was 1\m=.\22 \ m=+-\ 0\m=.\31. The comparisons indicated that the short procedure was the more reliable method at levels below 5 \ g=m\ g. / 24-hr. urine. Values for normal subjects are given. The methods appear to be entirely suitable for assessing oestrogen production by the ovaries, testes, adrenals and placenta.

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“…Urinary steroids were measured by conventional me¬ thods: pregnanediol and pregnanetriol by gas-chromatography (Scholler et al 1968), total 17ct-hydroxycorticosteroids and total 17-ketosteroids by colorimetry (Schol¬ ler et al 1957;Crepy et al 1956), total oestrogens by fluorometry (Scholler et al 1966). …”

Section: Methodsmentioning

confidence: 99%

17α-Hydroxylase deficiency syndrome associated with bilateral streak gonads and impaired development of Müllerian ducts derivatives

Gennes¹,

Jambart²,

Turpin³

et al. 1982

Acta Endocrinologica

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We report severe 17\g=a\-hydroxylase deficiency in a 17 year-old black girl with 46,XX genotype. The diagnosis was suspected because of primary amenorrhoea, absence of sexual maturation, hypertension and hypokalaemia with renal potassium wasting. Endocrine investigation revealed low basal levels of all steroid hormones which require 17\g=a\-hydroxylation for biosynthesis (i.e. glucocorticoids, androgens and oestrogens). No increase in their basal levels was seen following adrenal stimulation, indicating a severe block. Plasma concentrations of ACTH, FSH and LH were elevated as were progesterone, 11-deoxycorticosterone and corticosterone. Plasma renin activity was suppressed and aldosterone levels were very low. After 4 months of glucocorticoid replacement therapy, aldosterone was still low, even though the suppression was otherwise effective. Our case is unusual because bilateral streak gonads and impaired development of M\l=u"\llerianducts derivatives were also present. To our knowledge, a similar case has never been reported before. Steroid 17a-hydroxylation is an obligatory step in the biosynthesis of glucocorticoids, androgens and oestrogens ( Table 1). The 17a-hydroxylase defi¬ ciency syndrome (17-OHDS) is a very rare entity, first described by Biglieri et al. (1966) in a female patient and by New (1970) in a male patient. To date, no more than 25 patients have been reported. We report a female patient presenting typical signs of severe 17cx-hydroxylase deficiency, associated with bilateral streak gonads and impaired develop¬ ment of Müllerian duct derivatives. Case ReportThe patient was first seen at the age of 17 years because of primary amenorrhoea and complete lack of secondary sex development. She had been born after a normal pregnancy and delivery, and her infancy and childhood were uneventful. Her parents are not related and no family history of endocrine disease was found.Physical examination showed a black girl, 162 cm in height and 55 kg in weight. Breasts were undeveloped and no pubic or axillary hair was present. There was no webbing of the neck nor cubitus valgus. External geni¬ talia were those of an immature female. The vagina was short. On rectal examination, the uterus and the internal genitalia were not detected. The blood pressure was 170/120 mmHg in both arms and remained unchanged in the upright position. This hypertension was well tolerated.The bone age was determined to be 12 years. Films of the skull were normal. Films of the chest revealed a posteroinferior mediastinal ovoid mass. This mass showed a cystic appearance on CT-scan. The sodium was 143 mmol/1, potassium 3.2 mmol/1, chloride 103 mmol/1, bicarbonate 28 mmol/1, creatinine 62 u.mol/1, glucose 3.88 mmol/1, calcium 2.34 mmol/1, phosphorus 1.67 mmol/1 and alkaline phosphatase 191 IU/1. The urinary daily 1 To whom requests for reprints should be addressed. Service d'Endocrinologie Métabolisme, Hôpital de la Pitié, 83 boulevard de l'Hôpital, 75013 Paris

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A Study of Estrogen Fluorescence Using Ittrich's Procedure

Cited by 10 publications

References 0 publications

Further Observations on the Kober Colour and Ittrich Fluorescence Reactions in the Measurement of Oestriol, Oestrone and Oestradiol

Further Observations on the Kober Colour and Ittrich Fluorescence Reactions in the Measurement of Oestriol, Oestrone and Oestradiol

A Rapid Method for Estimating Oestrogens in Urine Using a Semi-Automatic Extractor

17α-Hydroxylase deficiency syndrome associated with bilateral streak gonads and impaired development of Müllerian ducts derivatives

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