The measured uniaxial-head load spectrum in the road simulation test has a large number of useless small loads. When applying the measured load spectrum directly, it will take a lot of time. This paper designs a comprehensive road spectrum measurement system to collect data and proposes a method for editing the uniaxial-head acceleration load spectrum using short-time Fourier transform to speed up the reliability test process and reduce time costs. In this method, the time domain and frequency domain information of the signal is obtained by short-time Fourier transform. The concept of accumulated power spectral density is proposed to identify the reduced load data, and the relative fatigue damage is used as the pass criterion. The length of the edited spectrum is only 66% of the original spectrum through the above-mentioned editing method and retains the relative damage amount of 91%. Finally, through the analysis of time domain, frequency domain, and fatigue statistical parameters, it demonstrates that the short-time Fourier transform–based acceleration load spectrum edition method could achieve a similar fatigue damage to the original spectrum in a shorter time.