A study of genetic variation and evolution of Phyllostachys (Bambusoideae: Poaceae) using nuclear restriction fragment length polymorphisms

Friar, Elizabeth A.; Kochert, Gary

doi:10.1007/bf00225152

Cited by 44 publications

(29 citation statements)

References 8 publications

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“…1997). The assertion that P. nigra belongs to section Heteroclada is also congruent with the results of a nuclear RFLP study by Friar and Kochert (1994). Phyllostachys aureosulcata is intermediate in morphology between sections Heteroclada and Phyllostachys and was placed by Renvoize and Hodkinson (1997) in a revised section Heteroclada on the basis of its morphology.…”

Section: Aflpsupporting

confidence: 73%

“…(1980). Friar and Kochert (1994) assessed variation at the DNA sequence level using restriction fragment length polymorphisms (RFLPs) and found that the taxa clustered into two broad groups when assessed with both genetic distance methods and phylogenetic methods using maximum parsimony. Their results were in broad agreement with the morphological classification of Wang et a/.…”

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confidence: 99%

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A Comparison of ITS Nuclear rDNA Sequence Data and AFLP Markers for Phylogenetic Studies in Phyllostachys (Bambusoideae, Poaceae)

et al. 2000

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Two contrasting molecular techniques, namely DNA sequences and amplified fragment length polymorphisms (AFLP) were used to investigate phylogenetic relationships of Phyllostachys, a large, economically important genus of woody bamboos. DNA sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) were used in a parsimony analysis. PhyrrOStachys was well supported as monophyletic with Chimonobambusa as its closest allied genus. The 5s spacer region of nrDNA was investigated but found unsuitable for this purpose. The AFLP analysis showed much higher discriminating power between species and was more useful for phylogenetic reconstruction at this taxonomic level. The combined data were used to review the previous infra-generic classifications. Section Heteroclada Wang & Ye is strongly supported and can be further divided into sub-groups. A group within section Phyllosachys is strongly supported, but a further group of taxa previously included in this section is difficult to place. The ability of the methods to help separate species such as P.sulphurea and investigate genetic diversity at the infra-specific level was also assessed. It is argued that AFLPs could often be the method of choice for phylogenetic studies of closely related taxa for which DNA sequence data provide insufficient resolution.

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Section: Aflpsupporting

confidence: 73%

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confidence: 99%

A Comparison of ITS Nuclear rDNA Sequence Data and AFLP Markers for Phylogenetic Studies in Phyllostachys (Bambusoideae, Poaceae)

et al. 2000

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“…The cluster analysis indicates that 12 taxa belonging to different genera of bamboo formed two major clusters based on similarity indices. Both major clusters were further divided into (Friar and Kochert 1994) and RAPD (Gielis et al 1997). Besides, the divergence was higher between the genus Sasa and Bambusa, Bambusa and Dendrocalamus.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the genetic variability in bamboo using RAPD markers

Nayak¹,

Rout²,

Das³

2003

PLANT SOIL ENVIRON

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Classical taxonomic studies of the bamboos are based on floral morphology and growth habit, which can cause problems in identification due to erratic flowering. Identification and genetic relationships in 12 species of bamboo were investigated using random amplified polymorphic DNAs (RAPD) technique. Analysis started by using thirty 10-mer primers that allowed us to distinguish 12 species and to select a reduced set of primers. The selected primers were used for identification and for establishing a profiling system to estimate genetic diversity. A total of one hundred thirty seven distinct polymorphic DNA fragments (bands), ranging from 0.4-3.3 kb were amplified by using 10 selected primers. The genetic similar analysis was conducted based on presence or absence of bands, which revealed a wide range of variability among the species. Cluster analysis clearly showed two major clusters belonging to 12 species of bamboo. Two major clusters were further divided into three minor clusters. The species of Bambusa vulgaris and Bambusa vulgaris var. striata were the most closely related and formed the first minor cluster along with Bambusa ventricosa. The variety of Bambusa multiplex var. Silver stripe and Bambusa multiplex were very closely related and there was no variation with Bambusa ventricosa. Another minor cluster was obtained between Bambusa arundinacea, Cephalostachyum pergracil and Bambusa balcooa. The RAPD technique has the potential for use in species identification and genetic relationships between taxa and species of bamboo for breeding program.

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“…Many molecular approaches, such as RAPD (Random Amplified Polymorphism DNA), AFLPs (Amplified Fragment Length Polymorphisms) and RFLPs (Restriction Fragment Length Polymorphisms) have been used in studies of bamboo genetic variability (Friar and Kochert 1994, Lai and Hsiao 1997, Hodkinson et a/. 2000.…”

mentioning

confidence: 99%

Genetic Variation and Evolution of the Alpine Bamboos (Poaceae: Bambusoideae) using DNA Sequence Data

Guo

Chen

et al. 2001

J Plant Res

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Difficulties in phylogenetic reconstruction are common within the woody bamboos due to their unique life cycles, a lack of morphological characters and even an absence of molecular evidence. The genetic variability and population structure of the bamboos are also poorly understood which impedes their exploitation via breeding. In this paper, ITS sequence data were used to examine the degree of genetic variation within the alpine bamboos and to explore their phylogeny. Twenty-three species representing three genera, Thamnocaamus, Fatgesia and Yushania, of the alpine bamboos and one species of Ampelocalamus as an outgroup were studied. The results indicated that mamnocalamus spathiflorus var. crassinodus and the Fatgesia spathacea clade form the basal groups but bootstrap support was weak. Among the rest of the species, including species previously placed in Fatgesia (plus Borinda) and Yushania, the F. yunnanensis subclade and the F. communis subclade were recognized but internal support for such groups was again low. The result indicated that, Fatgesia and Yushania as delimited by morphological characters, are not monophyletic in the ITS phylogeny and require further resolution. We revealed relatively high levels of genetic variability in the alpine bamboos and showed that the ITS region could be used to improve generic delimitation of the woody bamboos in general.

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A study of genetic variation and evolution of Phyllostachys (Bambusoideae: Poaceae) using nuclear restriction fragment length polymorphisms

Cited by 44 publications

References 8 publications

A Comparison of ITS Nuclear rDNA Sequence Data and AFLP Markers for Phylogenetic Studies in Phyllostachys (Bambusoideae, Poaceae)

A Comparison of ITS Nuclear rDNA Sequence Data and AFLP Markers for Phylogenetic Studies in Phyllostachys (Bambusoideae, Poaceae)

Evaluation of the genetic variability in bamboo using RAPD markers

Genetic Variation and Evolution of the Alpine Bamboos (Poaceae: Bambusoideae) using DNA Sequence Data

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