A study of high level aminoglycoside resistant enterococci

El-Ghazawy, Iman F.; Okasha, Hadir; Mazloum, Sarah M.

doi:10.5897/ajmr2015.7943

Cited by 8 publications

(2 citation statements)

References 20 publications

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“…In the present study, enterococci tested against commonly used antibiotics by the Kirby–Bauer disc diffusion method showed highest susceptibility for linezolid (97.8%), followed by teicoplanin (95.6%), high-level gentamicin (81.3%), and vancomycin (79.1%), which was similar to picture in other studies except, higher incidence of high-level gentamicin resistance (HLGR) than vancomycin resistance was noted in the previous studies [2, 22]. Low prevalence of resistance against vancomycin, teicoplanin, and linezolid was observed in a study from China [6].…”

Section: Discussionsupporting

confidence: 89%

“…Out of 56 urinary isolates, 76.7% isolates were resistant to ciprofloxacin. However, a higher and lower prevalence of ciprofloxacin resistant strains among urinary isolates was observed in other studies, respectively [1, 22]. The high resistance to ciprofloxacin probably could be because of its irrational usage as broad-spectrum antibiotic against a wide range of diseases.…”

Section: Discussionmentioning

confidence: 84%

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Characterization of Clinical Isolates ofEnterococciwith Special Reference to Glycopeptide Susceptibility at a Tertiary Care Center of Eastern Nepal

Karna

Baral

Khanal

2019

International Journal of Microbiology

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Background. Enterococci, once considered as a harmless commensal of intestine, have now emerged as medically important pathogens and are associated with both community-acquired and nosocomial infections. They bear the potential to exhibit resistance against all commonly used antibiotics either by inherent or acquired mechanism, posing a therapeutic challenge. Objectives. This study aimed to characterize enterococci up to the species level and study their antibiogram with special regard to vancomycin. Methods. A descriptive cross-sectional study was conducted in the Department of Microbiology, B.P. Koirala Institute of Health Sciences, Dharan, Nepal, from February to May 2017. A total of 91 enterococcal isolates recovered from clinical specimens were investigated in this study. Their identification and speciation were done according to standard microbiological guidelines. Kirby–Bauer disc diffusion technique was used to study antimicrobial susceptibility pattern, whereas minimum inhibitory concentration of vancomycin was determined by the agar dilution method, with reference to Clinical and Laboratory Standards Institute guidelines. Results. Seven different species of enterococci were isolated, E. faecalis and E. faecium accounting about 45% each. The other species encountered were E. avium, E. cecorum, E. dispar, E. durans, and E. raffinosus. Highest proportion of antimicrobial susceptibility was recorded for linezolid (97.8%), followed by teicoplanin (95.6%) and high-level gentamicin (81.3%). Sensitivity to vancomycin was seen in 79.1% isolates. Likewise, 82.1% of urinary strains were susceptible to nitrofurantoin. A total of 4 disparities were observed between the disc diffusion technique and agar dilution method in determining vancomycin resistance. Multidrug resistance was observed in 31.9% isolates. The overall prevalence of vancomycin-resistant enterococci based on the standard minimum inhibitory concentration method was 25.3%. Conclusions. Enterococcus faecalis and E. faecium were the predominant species in causing enterococcal infections. The alarming rise in prevalence of vancomycin and multidrug resistance strains warrants immediate, adequate, and efficient surveillance program to prevent and control its spread.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 84%

Characterization of Clinical Isolates ofEnterococciwith Special Reference to Glycopeptide Susceptibility at a Tertiary Care Center of Eastern Nepal

Karna

Baral

Khanal

2019

International Journal of Microbiology

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Detection of antibiotic resistance profiles and aminoglycoside-modifying enzyme (AME) genes in high-level aminoglycoside-resistant (HLAR) enterococci isolated from raw milk and traditional cheeses in Turkey

Özdemir

Tuncer

2020

Mol Biol Rep

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Vancomycin-Resistant Enterococci and Its Associated Risk Factors among HIV-Positive and -Negative Clients Attending Dessie Referral Hospital, Northeast Ethiopia

Ali¹,

Alemayehu

Dagnew

et al. 2018

International Journal of Microbiology

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Background Enterococci are becoming the most important public health concern and emerging as multidrug-resistant organisms around the world including Africa particularly in Ethiopia where there is a lack of availability of effective antimicrobial drugs. However, there is a paucity of data on the prevalence and associated risk factors of vancomycin-resistant enterococci in Ethiopia. Objective This study was aimed to assess the prevalence of vancomycin-resistant enterococci and its associated risk factors among HIV-positive and -negative clients. Methods A comparative cross-sectional study was conducted from February to May, 2017, on 300 participants at Dessie Referral Hospital. Data were gathered using a pretested structured questionnaire, stool samples were collected and inoculated on to bile esculin agar, and presumptive colonies were inoculated in brain-heart infusion broth containing 6.5% NaCl for selective identification of enterococci. Antibiotic susceptibility tests were done using the Kirby–Bauer disk diffusion method. Data were analyzed using SPSS version 22 software package. Results A total of 300 study participants were enrolled in this study, of which 57.7% were females with a mean age of 34.4, a range of 19–73 years. The overall prevalence of enterococci was 37.3%. The prevalence of VRE was 6.3%. From all isolates, the prevalence of VRE among HIV-positive and -negative clients was 5.9% and 7.4%, respectively. Resistance gentamicin, ampicillin, penicillin, and erythromycin was 37.5%, 34.8%, 34.8%, and 22.3%, respectively. Prevalence of multidrug resistance was (29.5%). Being low in hemoglobin content was significantly associated with VRE. Conclusion The high prevalence of VRE and multidrug-resistant enterococci in this study signals the emergence of VRE. Detection of VRE in this study indicates decreased antibiotic treatment options of multidrug-resistant enterococci. Therefore, there should be a need to perform continuous surveillance, rational use of antibiotics, and more detailed study using phenotypic and genotypic methods.

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A study of high level aminoglycoside resistant enterococci

Cited by 8 publications

References 20 publications

Characterization of Clinical Isolates ofEnterococciwith Special Reference to Glycopeptide Susceptibility at a Tertiary Care Center of Eastern Nepal

Characterization of Clinical Isolates ofEnterococciwith Special Reference to Glycopeptide Susceptibility at a Tertiary Care Center of Eastern Nepal

Detection of antibiotic resistance profiles and aminoglycoside-modifying enzyme (AME) genes in high-level aminoglycoside-resistant (HLAR) enterococci isolated from raw milk and traditional cheeses in Turkey

Vancomycin-Resistant Enterococci and Its Associated Risk Factors among HIV-Positive and -Negative Clients Attending Dessie Referral Hospital, Northeast Ethiopia

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