A study of housefly esterases by means of a sensitive colorimetric method

Asperen, K. Van

doi:10.1016/0022-1910(62)90074-4

Cited by 846 publications

(308 citation statements)

References 8 publications

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“…Nonspecific esterase alpha esterases (毩-esterases) and beta esterases ( 毬 -esterase) were determined according to the method of Asperen [16] using 毩-naphthyl acetate and 毬 -naphthyl acetate as substrates, respectively.…”

Section: Biochemical Assaymentioning

confidence: 99%

Control of Tetranychus urticae Koch by extracts of three essential oils of chamomile, marjoram and Eucalyptus

Afify

Ali

Turky

2012

Asian Pacific Journal of Tropical Biomedicine

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Section: Biochemical Assaymentioning

confidence: 99%

Control of Tetranychus urticae Koch by extracts of three essential oils of chamomile, marjoram and Eucalyptus

Afify

Ali

Turky

2012

Asian Pacific Journal of Tropical Biomedicine

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“…The homogenate (25 p1) from each homogenizing well then was transferred using an eight-channel pipettor into the corresponding well on a separate ELISA plate. Nonspecific esterase activity of the individual aphid homogenates then was tested, as described by van Asperen (1962), by measuring the production of a-naphthol from a-naphthyl butyrate. The assay mixture consisted of 25 mM a-naphthyl butyrate in 0.02 M sodium phosphate (pH 7.0).…”

Section: Greenbugmentioning

confidence: 99%

Characterization of General Esterases from Susceptible and Parathion-Resistant Strains of the Greenbug (Homoptera: Aphididae)

Ono

Richman

Siegfried

1994

Journal of Economic Entomology

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“…Assay was carried out by Gomori [14] and later modified by Van Asperen [15]. Enzyme reaction was initiated by adding 900 ll of 5 mM a-naphthyl acetate in Phosphate assay buffer (pH 7.0) to pre incubated 100 ll tissue extract and was incubated for 15 min at 27°C.…”

Section: Carboxyl Esterasementioning

confidence: 99%

Activity of Hydrolytic Enzymes in Various Regions of Normal Human Brain Tissue

2012

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The activity of six hydrolytic enzymescarboxyl esterase, acid phosphatase, alkaline phosphatase, b-galactosidase, b-glucosidase and b-hexosaminidase, were studied in different regions of the normal human brain tissue obtained at autopsy. Protein estimation and activities of the hydrolytic enzymes with respective substrates were assayed by spectrophotometric and spectroflourometric methods. Amongst the eight regions of the brain-frontal, parietal, occipital, temporal, thalamus, cerebellum and hippocampus, the pineal gland showed highest activity for all hydrolytic enzymes studied except for carboxyl esterase. Among six hydrolases studied, hexosaminidase exhibited highest activity in all regions of the human brain while alkaline phosphatase activity was the least amongst all regions studied. A majority of the enzymes studied showed higher activity in gray matter as compared to the white matter except acid phosphatase and b-glucosidase which exhibited higher activity in the white matter. The most significant finding in the present study was the high activity of all hydrolytic enzymes noted in the pineal gland as compared to all other regions of the human brain. Such a finding has not been hitherto reported earlier in human brain tissue samples. If the specific activities of these enzymes are to be considered as any functional index, then pineal gland may be more metabolically active tissue with respect to the hydrolytic function as compared to the other regions of the brain.

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A study of housefly esterases by means of a sensitive colorimetric method

Cited by 846 publications

References 8 publications

Control of Tetranychus urticae Koch by extracts of three essential oils of chamomile, marjoram and Eucalyptus

Control of Tetranychus urticae Koch by extracts of three essential oils of chamomile, marjoram and Eucalyptus

Characterization of General Esterases from Susceptible and Parathion-Resistant Strains of the Greenbug (Homoptera: Aphididae)

Activity of Hydrolytic Enzymes in Various Regions of Normal Human Brain Tissue

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